BV2 Cell Line


      Catalog number:EP-CL-0493

      • 1×10⁶ cells/vial
      - +
      Price: $461

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Origin and General Characteristics

      Cell Name BV2 Cell Line
      Organism Mus musculus, Mouse
      Tissue Brain
      Morphology Morphology microglial
      Growth Properties Mixed, Adherent And Suspension
      Biosafety Level 1

      Culture Conditions and Handling

      Complete Growth Medium MEM +10% FBS +1% P/S
      Subculturing Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
      Subcultivation Ratio 1:2-1:4
      Medium Renewal Every 2 to 3 days
      Cryopreservation Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase
      Culture Conditions Atmosphere: Air, 95%; CO2, 5%Temperature: 37℃

      Related Resources

      Recommendations for handling of cryopreserved cells

      submit review
      • Show all (0)
      • Reviews (0)
      • Q&A (0)
      ... Show All Show Less
      MSDS for ELISA ISO 9001

      Browsing History

        People Also Bought

        Apply for Free Shipping 24T ELISA Kit
        *Product Name:
        *Catalog Number:
        How do you know

        *Verification code: