Caco-2 Cell Line

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      Catalog number:EP-CL-0050

      Size:
      • 1×10⁶ cells/vial
      Qty:
      - +
      Price: $349

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      Origin and General Characteristics

      Cell Name Caco-2 Cell Line
      Organism Homo sapiens, Human
      Age 72 years
      Tissue Colon
      Morphology Epithelial
      Growth Properties Adherent
      Descriptions Upon reaching confluence, the cells express characteristics of enterocytic differentiation. Ref Caco-2 cells express retinoic acid binding protein I and retinol binding protein Ⅱ.
      Biosafety Level 1

      Culture Conditions and Handling

      Complete Growth Medium MEM +20% FBS +1% P/S
      Subculturing Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
      Subcultivation Ratio 1:2-1:4
      Medium Renewal Every 2 to 3 days
      Cryopreservation Freeze medium: 60% Basal medium+30% FBS+10% DMSO
      Storage temperature: Liquid nitrogen vapor phase
      Culture Conditions Atmosphere: Air, 95%; CO2, 5%
      Temperature: 37℃

      Special Features of the Cell Line

      Tumorigenic Yes
      Effects Yes, in nude mice forms moderately well differentiated adenocarcinoma consistent with colonic primary(gradeⅡ).
      Receptor Expression This cell line expressed heat stable enterotoxin(Sta, E.coli) and epidermal growth factor(EGF).
      Gene Expression Keratin, Retinoic Acid Binding Protein 1, Retinol Binding Protein 2
      Applications Transfection host.

      Related Resources

      Recommendations for handling of cryopreserved cells

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