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Cell Cycle Assay Kit (Red Fluorescence)

  • Cat.No.:E-CK-A351

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Detection principle

Cell cycle refers to the whole process from the end of one mitosis to the end of the next. During this process, the genetic material is replicated and doubled, and evenly distributed to two daughter cells at the end of division. Cell cycle can also be divided into phases like interphase and Metaphase. Intercellular phase can also be divided into dormancy (zero gap, G0), prophase of DNA synthesis (first gap, G1), anaphase of DNA synthesis (synthesis, S) and anaphase of DNA synthesis (second gap, G2). DNA detection can be used to reflect the status of each phase of the cell cycle which is also named cell proliferation. DNA can bind to fluorescent dyes (such as propidium Iodide-PI), the fluorescent dyes binding to DNA at different stages are different, and the fluorescence intensity detected by flow cytometry can also be used to detect different phases in cell cycle. When apoptotic cells occur, apoptotic bodies are produced due to the concentration and nucleolysis of cytoplasm and chromatin, which will change the light scattering properties of cells. In the early stage of apoptosis, the forward scattering (FSC) of cells decreased significantly, while the side scatter (SSC) increased or remained unchanged. In the late stage of apoptosis, both FSC and SSC signals decreased. Therefore, apoptotic cells can be observed by measuring the changes of light scattering by flow cytometry. After staining with PI, assuming that the fluorescence intensity of G0/G1 phase cells is 1, the theoretical value of fluorescence intensity of G2/M phase cells containing double genomic DNA is 2, and the fluorescence intensity of S phase cells undergoing DNA replication is between 1 and 2. Apoptotic cells lost part of genomic DNA fragmentation due to nucleus concentration and DNA fragmentation. Therefore, apoptotic cells showed obvious weak staining after PI staining and the fluorescence intensity was less than 1. The sub-G1 peak appeared on the flow cytometry fluorescence map which is apoptotic cell. The kit can be used to detect the DNA content (cell cycle) of cultured cells (suspension or adherence cells).

General Information

Application Cell Cycle
Detection method Fluorometric Method
Sample type Cell samples
Assay time 3 hours
Detection instrument Flow Cytometer
Dye Type PI
Ex/Em (nm) 536/617
Channel set PI,PerCP,PE
Other reagents required Ethanol, PBS(E-IR-R187)
Storage 2~8°C/-20°C, shading light. Refer to the Manual.
Shipping Ice bag
Expiration date 12 months

Citations

  1. WATER RESEARCH (2020) IF: 9.1300
    Comparative toxicity reduction potential of UV/sodium percarbonate and UV/hydrogen peroxide treatments for bisphenol A in water: An integrated analysis using chemical, computational, biological, and metabolomic approaches

    DOI: 10.1016/j.watres.2020.116755

    PMID: 33383346

  2. Molecular Therapy-Nucleic Acids (2020) IF: 7.032
    Transient transfection of the respiratory epithelium with gamma interferon for host-directed therapy in pulmonary tuberculosis

    DOI: 10.1016/j.omtn.2020.10.023

    PMID: 33110704

    Sample: Tissue Slice
  3. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 7.419
    Efficacy of WWQ-131, a highly selective JAK2 inhibitor, in mouse models of myeloproliferative neoplasms

    DOI: 10.1016/j.biopha.2022.113884

    PMID: 36306591

  4. BIOFACTORS (2021) IF: 6.113
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    DOI: 10.1002/biof.1791

    PMID: 34669997

  5. JOURNAL OF CELLULAR PHYSIOLOGY (2022) IF: 6.513
    IGF2BP2, an RNA-binding protein regulates cell proliferation and osteogenic differentiation by stabilizing SRF mRNA

    DOI: 10.1002/jcp.30919

  6. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2023) IF: 5.19
    N-acetylcysteine rescues meiotic arrest during spermatogenesis in mice exposed to BDE-209

    DOI: 10.1007/s11356-023-25874-0

  7. BIOORGANIC CHEMISTRY (2023) IF: 5.307
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    DOI: 10.1016/j.bioorg.2023.106401

  8. JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY (2023) IF: 5.756
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    DOI: 10.1080/14756366.2022.2155815

  9. Pharmaceuticals (2021) IF: 5.863
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    DOI: 10.3390/ph14121287

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    DOI: 10.1002/tox.23488

  11. TOXICOLOGY AND APPLIED PHARMACOLOGY (2022) IF: 4.219
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    DOI: 10.1016/j.taap.2022.115926

    PMID: 35182550

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    DOI: 10.1016/j.taap.2021.115834

    PMID: 34933054

  13. TOXICOLOGY (2021) IF: 4.221
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    DOI: 10.1016/j.tox.2021.153061

    PMID: 34936917

  14. TOXICOLOGY AND APPLIED PHARMACOLOGY (2022) IF: 4.46
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    DOI: 10.1016/j.taap.2022.116326

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    DOI: 10.1016/j.taap.2022.116232

    PMID: 36089000

  16. BMC CANCER (2022) IF: 4.638
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    DOI: 10.1186/s12885-022-09884-8

    PMID: 35879690

  17. RSC Advances (2021) IF: 3.361
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    DOI: 10.1039/D1RA03640H

    PMID: 35497558

  18. PLoS One (2022) IF: 3.752
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    DOI: 10.1371/journal.pone.0272499

    PMID: 36067193

  19. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY (2022) IF: 3.981
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    DOI: 10.1016/j.jddst.2022.103497

  20. TISSUE & CELL (2022) IF: 2.466
    Deactivation of AKT/GSK-3β-mediated Wnt/β-catenin pathway by silencing of KIF26B weakens the malignant behaviors of non-small cell lung cancer

    DOI: 10.1016/j.tice.2022.101750

    PMID: 35182988

  21. Evidence-based Complementary and Alternative Medicine (2022) IF: 2.65
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    DOI: 10.1155/2022/5481563

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Reviews/Q&A

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  • Reviews
  • Q&A
Q

N****************mSubmitted [ Jan 31 2024 ]

Asked: Inquiry Regarding Cell Cycle Assay Kits and Compatible Flow Cytometry Instruments. Dear Technical Support Team, I hope this email finds you well. I recently purchased the Cell Cycle Assay Kit (Red Fluorescence) and am eager to perform cell cycle analysis using your product. While reviewing the available options, I noticed that there are also kits with green and blue fluorescence. Could you kindly explain the differences between these three kits? Additionally, I am inquiring about the recommended flow cytometry instruments compatible with your Cell Cycle Assay Kits. Could you provide information on which brand of flow cytometry instrument is best suited for optimal results with your kits? I have a specific question regarding the use of your kit with BD machines. According to BD\'s recommendations, the use of CEN and CTN reagents is advised. However, upon reviewing the Cell Cycle Assay Kit (Red Fluorescence), I did not find information about these reagents, nor was it mentioned to use both. Could you please offer guidance on whether the CEN and CTN reagents are necessary for the effective use of your Cell Cycle Assay Kit in conjunction with BD machines? Thank you in advance for your assistance. I appreciate your prompt response and valuable insights.

Reply

A

adminSubmitted [ Jan 31 2024 ]

Answered: We'll reply you by emil soon. :)

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