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Choline Acetyltransferase (ChAT) Activity Assay Kit (Tissue Samples)

  • Cat.No.:E-BC-K125-S

  • Detection instrument: Spectrophotometer(324 nm)

To Purchase E-BC-K125-S

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Detection principle

Acetyl-CoA can react with choline under the catalysis of choline acetyltransferase (ChAT) to produce coenzyme A (CoA), CoA can combine with the 4, 4-dithiopyridine. The activity of ChAT can be calculated indirectly by measuring the OD value at 324 nm.

Performance characteristics

Synonyms ChAT
Sample type Animal tissue
Sensitivity 1.21 U/g fresh weigh
Detection range 1.21-40 U/g fresh we
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 80 min
Precision Average inter-assay CV: 9.200%Average intra-assay CV: 5%
Other instruments required Micropipettor, Centrifuge, Incubator, Water bath, Vortex mixer
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage Reagent 2, Reagent 3, Reagent 6: -20℃, others: 2-8℃
Valid period 12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (1.21-40 U/g fresh weight).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

20% Mouse brain tissue homogenate

1-2

20% Rat heart tissue homogenate

1

20% Rat liver tissue homogenate

1

20% Mouse kidney tissue homogenate

1

20% Rat lung tissue homogenate

1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

  1. Antioxidants (2021) IF: 6.313
    Central Administration of Ampelopsin A Isolated from Vitis vinifera Ameliorates Cognitive and Memory Function in a Scopolamine-Induced Dementia Model

    DOI: 10.3390/antiox10060835

    PMID: 34073796

    Sample: Tissue homogenate
  2. Antioxidants (2020) IF: 5.014
    Enteromorpha prolifera Extract Improves Memory in Scopolamine-Treated Mice via Downregulating Amyloid-β Expression and Upregulating BDNF/TrkB Pathway

    DOI: 10.3390/antiox9070620

    PMID: 32679768

    Sample: Tissue homogenate
  3. NEUROCHEMICAL RESEARCH (2022) IF: 4.414
    The Role of Acetylcholine on the Effects of Different Doses of Sulfite in Learning and Memory

    DOI: 10.1007/s11064-022-03684-z

    PMID: 35895153

    Sample: Hippocampus tissue
  4. Processes (2023) IF: 3.352
    Memory-Improving Activity of the Flower Extract from Chrysanthemum boreale (Makino) Maskino in Scopolamine-Treated Rodents

    DOI: 10.3390/pr11010159

    Sample: Hippocampal
  5. Advances in Traditional Medicine (2022)
    Nootropic effect of Syzygium polyanthum (Wight) Walp leaf extract in chronic cerebral hypoperfusion rat model via cholinergic restoration: a potential therapeutic agent for dementia

    DOI: 10.1007/s13596-022-00653-3

    Sample: brain
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Reviews/Q&A

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  • Reviews
  • Q&A
Q

P***********lSubmitted [ Jul 14 2023 ]

Asked: what is the preheating temperature for working solution that you had mentioned in the procedure?

Reply

A

adminSubmitted [ Jul 14 2023 ]

Answered: Hello Parvati,thanks for your support.The preheating temperature for working solution that you had mentioned in the procedure is 37℃.

Q

M**********dSubmitted [ Aug 25 2022 ]

Asked: Is the kit suitable for cell?

Reply

A

adminSubmitted [ Aug 25 2022 ]

Answered: Yes, it takes about 3*10^6 cells with 200 micro-liter of saline or PBS based on the cell types.

Q

D********dSubmitted [ Aug 03 2022 ]

Asked: Why can\'t I see any color development after the whole process?

Reply

A

adminSubmitted [ Aug 03 2022 ]

Answered: The detection wavelength is 324 nm which is in the range of ultra-violet, thus people cannot see it but the absorbance can be detected by the instrument.

Q

b***lSubmitted [ May 23 2019 ]

Asked: how can i prepare Boiled sample?

Reply

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