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Citric Acid (CA) Colorimetric Assay Kit

Cat.No.:E-BC-K351-S
Detection instrument: Spectrophotometer(545 nm)

Detection method: Colorimetric method

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Detection principle

In acidic condition, Cr (Ⅵ) will be reduced to Cr3+, Cr3+ reacts with citric acid. And the product has a characteristic absorption peak at 545 nm, therefore the content of citric acid in sample can be calculated by measuring the absorbance value at 545 nm.

Performance characteristics

Synonyms	CA
Sample type	Tissue,mitochondria,other liquid samples
Sensitivity	0.05 mmol/L
Detection range	0.05-5.0 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	60 min
Precision	Average inter-assay CV: 5.400%Average intra-assay CV: 4.200%
Other instruments required	Micropipettor, Water bath, Incubator, Vortex mixer, Centrifuge
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.05-5.0 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	4-6
10% Mouse kidney tissue homogenate	2-4
10% Rat kidney tissue homogenate	1
10% Mouse heart tissue homogenate	2-4
10% Mouse brain tissue homogenate	1
10% Mouse liver tissue homogenate	1

Note: The diluent is reagent 1.

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Collection and treatment of common samples for metabolism assay detection

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