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Creatine kinase (CK) Activity Assay Kit

  • Cat.No.:E-BC-K558-S

  • Detection instrument: Spectrophotometer(340 nm)

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Detection principle

Creatine kinase (CK) catalyze creatine phosphate and ADP to produce creatine and ATP. Hexokinase catalyze creatine and glucose to produce glucose-6-phosphate. Glucose-6-phosphate dehydrogenase (G-6-PD) catalyze glucose-6-phosphate and NADP+ to produce NADPH which have a maximum absorption peak at 340 nm. The CK activity can be calculated by measuring the OD values at 340 nm.

Performance characteristics

Synonyms CK,Creatine phosphokinase
Sample type Serum,plasma,animal tissue,cells
Sensitivity 3.7 U/L
Detection range 3.7-160 U/L
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 80 min
Precision Average inter-assay CV: 8.100%Average intra-assay CV: 5.200%
Other instruments required Micropipettor, Incubator, Vortex mixer
Other reagents required PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range  (3.7-160 U/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Human plasma

1

Mouse serum

1

Rat serum

1

HepG2 cells homogenization

1

10% Rat kidney tissue homogenization

1

10% Rat brain tissue homogenization

2-5

10% Rat liver tissue homogenization

2-10

Note: The diluent is  PBS (0.01 M, pH 7.4).

Citations

  1. EUROPEAN JOURNAL OF PHARMACOLOGY (2021) IF: 4.432
    Glycyrrhizic acid ameliorates myocardial ischemia-reperfusion injury in rats through inhibiting endoplasmic reticulum stress

    DOI: 10.1016/j.ejphar.2021.174353

    PMID: 34274339

    Sample: serum
  2. MICROVASCULAR RESEARCH (2023) IF: 3.75
    Co-administration of metformin and/or glibenclamide with losartan reverse NG-nitro-l-arginine-methyl ester-streptozotocin-induced hypertensive diabetes and haemodynamic sequelae in rats

    DOI: 10.1016/j.mvr.2023.104497

    Sample: Serum
  3. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2019) IF: 2.928
    Protective effect of Barbaloin in a rat model of myocardial ischemia reperfusion injury through the regulation of the CNPY2‑PERK pathway

    DOI: 10.3892/ijmm.2019.4123

    Sample: Serum
  4. GENE (2020) IF: 2.984
    Puerarin alleviates coronary heart disease via suppressing inflammation in a rat model

    DOI: 10.1016/j.gene.2020.145354

    PMID: 33333215

    Sample: blood
  5. Journal of Nanomaterials (2021) IF: 2.986
    Effect of Hafnium Coating on Osseointegration of Titanium Implants: A Split Mouth Animal Study

    DOI: 10.1155/2021/7512957

    Sample: mandibular bone
  6. Molecular Medicine Reports (2016) IF: 1.559
    Protective effects of ghrelin against oxidative stress, inducible nitric oxide synthase and inflammation in a mouse model of myocardial ischemia/reperfusion injury via the HMGB1 and TLR4/NF-κB pathway

    DOI: 10.3892/mmr.2016.5535

    Sample: serum
  7. BIOMEDICAL AND ENVIRONMENTAL SCIENCES (2019) IF: 1.917
    Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient Mice

    DOI: 10.3967/bes2018.101

    Sample: Plasma,Tissue homogenate
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