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Cat.No.:E-BC-K558-S
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Protocols for metabolism assays
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Synonyms | CK,Creatine phosphokinase |
Sample type | Serum,plasma,animal tissue,cells |
Sensitivity | 3.7 U/L |
Detection range | 3.7-160 U/L |
Detection method | Colorimetric method |
Assay type | Enzyme Activity |
Assay time | 80 min |
Precision | Average inter-assay CV: 8.100%Average intra-assay CV: 5.200% |
Other instruments required | Micropipettor, Incubator, Vortex mixer |
Other reagents required | PBS (0.01 M, pH 7.4) |
Storage | 2-8℃ |
Valid period | 12 months |
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (3.7-160 U/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Human serum |
1 |
Human plasma |
1 |
Mouse serum |
1 |
Rat serum |
1 |
HepG2 cells homogenization |
1 |
10% Rat kidney tissue homogenization |
1 |
10% Rat brain tissue homogenization |
2-5 |
10% Rat liver tissue homogenization |
2-10 |
Note: The diluent is PBS (0.01 M, pH 7.4).
PMID: 34274339 |
Sample: serum |
Sample: Serum |
Sample: Serum |
PMID: 33333215 |
Sample: blood |
DOI: 10.1155/2021/7512957 |
|
Sample: mandibular bone |
Sample: serum |
DOI: 10.3967/bes2018.101 |
|
Sample: Plasma,Tissue homogenate |