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D-Hank's Balanced Salt Solution (D-HBSS), without calcium, magnesium, phenol red

  • Cat.No.:PB180321

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  • 500mL
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Background

Balanced Salt Solution (Physiological Solution) have the properties of buffer solution (regulate pH), normal saline (maintain osmotic pressure) and culture medium (provide nutrition). It can meet the basic needs of survival and metabolism of tissues, organs or cells in vitro. A small amount of phenolic red was added to some equilibrium salt solutions to indicate the pH change of the solution. Hank's Balanced Salt Solution (HBSS) is one of the commonly used phosphate buffers in cell separation or culture. The main components are NaCl、KCl、KH2PO4、Na2HPO4、NaHCO3 and glucose. HBSS is used for the washing of cells before passage and tissue dissociation, preparation of cell dissociation reagent, transport of cell or tissue, dilution of cells, etc.

General Information

Product Form Liquid
Concentration
pH 7.2-7.4
D-Glucose 1000mg/L
NaHCO3 350mg/L
Phenol red negative
Ca2+-Mg2+ Negative
Storage 2-30°C
Shipping RT
Expiration date 36 months

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Notes

1. This product is for research use only.
2. This product is sterilized by 0.22 μ m filtration and use directly without dilution.
3. It is necessary to pay attention to the aseptic operation and avoid the pollution.
4. The concentration of NaHCO3 in Hank's Balanced Salt Solution is very low and it is not suitable for 5% CO2 environment. Otherwise, the solution will change to acid quickly.

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