Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> Elab Fluor® Violet 450 Anti-Mouse CD5 Antibody[53-7.3]
Cat.No.:E-AB-F1185Q
Welcome to order from local distributors.
FAQ for Flow Cytometry Antibody
Add to cart Bulk requestFor research use only. Order now, ship in 3 days
Alternate Names | Ly-1,Lyt-1,Lymphocyte antigen 1,CD5,Cd5 |
Swissprot | P13379 |
Gene ID | 12507 |
References |
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kipps TJ. 1988. Adv. Immunol. 47:117. 3. Antin JH, et al. 1985. J. Immunol. 136:505. 4. Tarakhovsky A, et al. 1995. Science 269:535. |
Form | Liquid |
Clone No. | 53-7.3 |
Host | Rat |
Isotype | Rat IgG2a, κ |
Isotype Control | Elab Fluor® Violet 450 Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09832Q] |
Reactivity | Mouse |
Application | FCM |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | Elab Fluor® Violet 450 |
Elab Fluor® Violet 450 is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450 nm (e.g., a 450/45 nm bandpass filter). | |
How to select the appropriate detection channel through the spectrogram?
CD5 is a 67 kD protein, also known as Lyt-1, Ly-1, T1, Tp67, or Ly-12. It is a member of the scavenger receptor cysteine-rich protein superfamily (SRCR) and primarily expressed on thymocytes, T cells, and B-1 cells. Although mature α/β T cells express high levels of CD5, very few γ/δ T cells express this antigen. The interaction of CD5 with CD72, gp35-37, TCR, or BCR is involved in T and B cell activation. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |