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Human IL-8(Interleukin 8) ELISA Kit

  • Cat.No.:E-EL-H6008

  • Reactivity: Human

To Purchase E-EL-H6008

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 7.81-500 pg/mL
Sensitivity 4.69 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human IL-8 in samples. No significant cross-reactivity or interference between Human IL-8 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human IL-8 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-8. You can calculate the concentration of Human IL-8 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-8 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-8 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 20.34 60.67 245.62 24.62 56.15 233.52
Standard deviation 1.21 2.80 12.26 1.39 2.80 9.69
CV (%) 5.95 4.62 4.99 5.65 4.99 4.15

Recovery

The recovery of Human IL-8 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-99 94
EDTA plasma (n=8) 95-102 98
Cell culture media (n=8) 98-106 102

Linearity

Samples were spiked with high concentrations of Human IL-8 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P10145
SynonymsIL8, CXCL8, GCP-1, GCP1, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, 3-10C, AMCF-I, K60
Research AreaCancer, Cardiovascular, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. CELL (2020) IF: 38.637
    Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output

    DOI: 10.1016/j.cell.2020.08.009

    PMID: 33015716

    Sample: Cell culture supernatant
  2. Theranostics (2021) IF: 11.556
    A SCID mouse-human lung xenograft model of SARS-CoV-2 infection

    DOI: 10.7150/thno.58321

    PMID: 33995679

    Sample: Tissue homogenate
  3. Journal of Neuroinflammation (2022) IF: 9.587
    Transient post-operative overexpression of CXCR2 on monocytes of traumatic brain injury patients drives monocyte chemotaxis toward cerebrospinal fluid and enhances monocyte-mediated immunogenic cell death of neurons in vitro

    DOI: 10.1186/s12974-022-02535-6

    PMID: 35768823

    Sample: cerebrospinal fluid (CSF),peripheral monocytes
  4. Cell Death & Disease (2022) IF: 8.4689
    A novel mechanism for A-to-I RNA-edited AZIN1 in promoting tumor angiogenesis in colorectal cancer

    DOI: 10.1038/s41419-022-04734-8

    PMID: 35365616

    Sample: HCT-116 cell,HT-29 cell
  5. CANCER LETTERS (2022) IF: 8.679
    Decreased CXCR2 expression on circulating monocytes of colorectal cancer impairs recruitment and induces Re-education of tumor-associated macrophages

    DOI: 10.1016/j.canlet.2022.01.004

    PMID: 34999169

    Sample: serum,THP-1 cell,human circulating CD14+ monocytes
  6. Frontiers in Immunology (2022) IF: 8.786
    METTL3 inhibits inflammation of retinal pigment epithelium cells by regulating NR2F1 in an m6A-dependent manner

    DOI: 10.3389/fimmu.2022.905211

    PMID: 35936005

    Sample: RPE cell
  7. BRITISH JOURNAL OF CANCER (2022) IF: 7.64
    Cathepsin F and Fibulin-1 as novel diagnostic biomarkers for brain metastasis of non-small cell lung cancer

    DOI: 10.1038/s41416-022-01744-3

    PMID: 35217799

    Sample: serum
  8. EBioMedicine (2018) IF: 6.183
    The enhancer RNA lnc-SLC4A1-1 epigenetically regulates unexplained recurrent pregnancy loss (URPL) by activating CXCL8 and NF-kB pathway

    DOI: 10.1016/j.ebiom.2018.11.015

    PMID: 30448228

    Sample: Cell culture supernatant
  9. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    In Vitro Evaluation of Anti-Inflammatory and Protective Potential of an Extract from Cornus mas L. Fruit against H2O2-Induced Oxidative Stress in Human Skin Keratinocytes and Fibroblasts

    DOI: 10.3390/ijms232213755

  10. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    Fermented Taiwanofungus camphoratus Extract Ameliorates Psoriasis-Associated Response in HaCaT Cells via Modulating NF-𝜅B and mTOR Pathways

    DOI: 10.3390/ijms232314623

  11. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2021) IF: 6.291
    Novel insights into the role of BRD4 in fine particulate matter induced airway hyperresponsiveness

    DOI: 10.1016/j.ecoenv.2021.112440

    PMID: 34175826

    Sample: hASMCs cells
  12. Food & Function (2022) IF: 6.317
    Whole grain germinated brown rice regulates intestinal immune homeostasis and gastrointestinal hormones in type 2 diabetic patients—a randomized control trial

    DOI: 10.1039/D2FO00477A

    Sample: serum
  13. Pharmaceutics (2022) IF: 6.525
    In Vitro Antibacterial and Anti-Inflammatory Activity of Arctostaphylos uva-ursi Leaf Extract against Cutibacterium acnes

    DOI: 10.3390/pharmaceutics14091952

    PMID: 36145700

    Sample: HaCaT cell
  14. Cancers (2023) IF: 6.575
    Salivary Interleukin Levels in Oral Squamous Cell Carcinoma and Oral Epithelial Dysplasia: Findings from a Sri Lankan Study

    DOI: 10.3390/cancers15051510

    Sample: Saliva
  15. PHYTOMEDICINE (2022) IF: 6.656
    Magnoflorine attenuates inflammatory responses in RA by regulating the PI3K/Akt/NF-κB and Keap1-Nrf2/HO-1 signalling pathways in vivo and in vitro

    DOI: 10.1016/j.phymed.2022.154339

    PMID: 35870375

    Sample: MH7A cell
  16. Frontiers in Cell and Developmental Biology (2020) IF: 6.684
    EZH2-Inhibited MicroRNA-454-3p Promotes M2 Macrophage Polarization in Glioma

    DOI: 10.3389/fcell.2020.574940

    PMID: 33363140

    Sample: Tissue homogenate
  17. CELL PROLIFERATION (2019) IF: 5.039
    Inhibition of CREB‐mediated ZO‐1 and activation of NF‐κB‐induced IL‐6 by colonic epithelial MCT4 destroys intestinal barrier function

    DOI: 10.1111/cpr.12673

    Sample: CaCO2 cells,HT‐29 cell
  18. CHEMICO-BIOLOGICAL INTERACTIONS (2021) IF: 5.194
    A novel glyceroglycolipid from brown algae Ishige okamurae improve photoaging and counteract inflammation in UVB-induced HaCaT cells

    DOI: 10.1016/j.cbi.2021.109737

    PMID: 34740599

    Sample: HaCaT cells
  19. CLINICAL SCIENCE (2020) IF: 5.223
    Effects of circulating extracellular microvesicles from spinal cord-injured adults on endothelial cell function

    DOI: 10.1042/CS20200047

    PMID: 32219341

    Sample: Cell culture supernatant
  20. Scientific Reports (2016) IF: 5.228
    TLR3-/4-Priming Differentially Promotes Ca 2+ Signaling and Cytokine Expression and Ca 2+ -Dependently Augments Cytokine Release in hMSCs

    DOI: 10.1038/srep23103

    Sample: Cell culture supernatant
  21. OSTEOARTHRITIS AND CARTILAGE (2018) IF: 5.454
    Associations between knee structural measures, circulating inflammatory factors and MMP13 in patients with knee osteoarthritis

    DOI: 10.1016/j.joca.2018.05.003

    Sample: Serum
  22. JOURNAL OF CELLULAR PHYSIOLOGY (2020) IF: 5.546
    Cytokine physiognomies of MSCs from varied sources confirm the regenerative commitment post-coculture with activated neutrophils

    DOI: 10.1002/jcp.29713

    PMID: 32385929

    Sample: Cell culture supernatant
  23. ENVIRONMENTAL POLLUTION (2019) IF: 5.714
    Activation of NF-κB pathways mediating the inflammation and pulmonary diseases associated with atmospheric methylamine exposure

    DOI: 10.1016/j.envpol.2019.06.059

    PMID: 31252119

    Sample: Cell culture supernatant
  24. Frontiers in Pharmacology (2022) IF: 5.811
    Inflammation Affects Liver Function and the Metabolism of Voriconazole to Voriconazole-N-Oxide in Adult and Elderly Patients

    DOI: 10.3389/fphar.2022.835871

    PMID: 35462904

    Sample: plasma
  25. COLLOIDS AND SURFACES B-BIOINTERFACES (2023) IF: 5.999
    Multifunctional barrier membranes promote bone regeneration by scavenging H2O2, generating O2, eliminating inflammation, and regulating immune response

    DOI: 10.1016/j.colsurfb.2023.113147

    Sample: periodontal ligament stem cells (PDLSCs)
  26. Pediatric Obesity (2021) IF: 4
    The effects of Mediterranean diet on hepatic steatosis, oxidative stress, and inflammation in adolescents with non-alcoholic fatty liver disease: A randomized controlled trial

    DOI: 10.1111/ijpo.12872

    PMID: 34881510

    Sample: serum
  27. ORAL DISEASES (2022) IF: 4.068
    Lycium barbarum polysaccharide-glycoprotein promotes osteogenesis in hPDLSCs via ERK activation

    DOI: 10.1111/odi.14409

    Sample: hPDLSCs
  28. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021) IF: 4.223
    Occupational exposure of rural workers to pesticides in a vegetable-producing region in Brazil

    DOI: 10.1007/s11356-021-12444-5

    PMID: 33469792

    Sample: Plasma
  29. Frontiers in Pharmacology (2020) IF: 4.225
    Sodium Tanshinone II Sulfonate A Ameliorates Hypoxia-Induced Pulmonary Hypertension

    DOI: 10.3389/fphar.2020.00687

    PMID: 32508639

    Sample: Tissue homogenate
  30. Frontiers in Pharmacology (2020) IF: 4.225
    NRF2 -617 C/A Polymorphism Impacts Proinflammatory Cytokine Levels, Survival, and Transplant-Related Mortality After Hematopoietic Stem Cell Transplantation in Adult Patients Receiving Busulfan-Based Conditioning Regimens

    DOI: 10.3389/fphar.2020.563321

    PMID: 33384597

    Sample: plasma
  31. International Journal of Nanomedicine (2017) IF: 4.3
    Zinc oxide nanoparticle-induced atherosclerotic alterations in vitro and in vivo

    DOI: 10.2147/IJN.S134897

    Sample: Cell culture supernatant,Serum
  32. DRUG DEVELOPMENT RESEARCH (2021) IF: 4.36
    CircTHBS1 facilitates the progression of interstitial cystitis depending on the regulation of miR-139-5p/MFN2 axis

    DOI: 10.1002/ddr.21864

    PMID: 34368980

    Sample: serum
  33. JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
    Anti-proliferation and anti-inflammation effects of corilagin in rheumatoid arthritis by downregulating NF-κB and MAPK signaling pathways

    DOI: 10.1016/j.jep.2021.114791

    PMID: 34737112

    Sample: MH7A cell
  34. JOURNAL OF PERIODONTAL RESEARCH (2022) IF: 4.419
    Slit guidance ligand 2 promotes the inflammatory response of periodontitis through activation of the NF-κB signaling pathway

    DOI: 10.1111/jre.12987

    PMID: 35426130

    Sample: Human gingival fibroblasts (hGFs)
  35. Nutrients (2020) IF: 4.546
    Effect of Lactobacillus acidophilus Fermented Broths Enriched with Eruca sativa Seed Extracts on Intestinal Barrier and Inflammation in a Co-Culture System of an Enterohemorrhagic Escherichia coli and Human Intestinal Cells

    DOI: 10.3390/nu12103064

    PMID: 33036498

    Sample: Cell culture supernatant
  36. Frontiers in Genetics (2022) IF: 4.599
    ILF2 Contributes to Hyperproliferation of Keratinocytes and Skin Inflammation in a KLHDC7B-DT-Dependent Manner in Psoriasis

    DOI: 10.3389/fgene.2022.890624

    Sample: keratinocytes
  37. International Journal of Environmental Research and Public Health (2022) IF: 4.614
    A Randomized Controlled Trial Evaluating the Levels of the Biomarkers hs-CRP, IL-6, and IL-8 in Patients with Temporomandibular Disorder Treated with LLLT, Traditional Conservative Treatment, and a Combination of Both

    DOI: 10.3390/ijerph19158987

    PMID: 35897358

    Sample: serum
  38. Frontiers in Genetics (2022) IF: 4.772
    Downregulation of miRNA miR-1305 and upregulation of miRNA miR-6785-5p may be associated with psoriasis

    DOI: 10.3389/fgene.2022.891465

    Sample: NHEK cell
  39. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2019) IF: 4.784
    Surface-layer protein from Lactobacillus acidophilus NCFM attenuates tumor necrosis factor-α-induced intestinal barrier dysfunction and inflammation

    DOI: 10.1016/j.ijbiomac.2019.06.041

    Sample: Caco-2 cells
  40. Biomolecules (2021) IF: 4.879
    Grape Pomace for Topical Application: Green NaDES Sustainable Extraction, Skin Permeation Studies, Antioxidant and Anti-Inflammatory Activities Characterization in 3D Human Keratinocytes

    DOI: 10.3390/biom11081181

    PMID: 34439847

    Sample: HaCaT keratinocytes
  41. AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2019) IF: 3.091
    Nisin reduces uterine inflammation in rats by modulating concentrations of pro‐ and anti‐inflammatory cytokines

    DOI: 10.1111/aji.13096

    Sample: Serum
  42. Oncology Letters (2022) IF: 3.111
    A role for serum cytokines and cell adhesion molecules in the non‑invasive diagnosis of colorectal cancer

    DOI: 10.3892/ol.2022.13443

    Sample: serum
  43. Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2021) IF: 3.168
    Inflammatory Biomarkers Levels in T2DM Emirati Patients with Diabetic Neuropathy

    DOI: 10.2147/DMSO.S319863

    PMID: 34345175

    Sample: serum
  44. FEBS LETTERS (2015) IF: 3.169
    The lncRNA MALAT1 protects the endothelium against ox‐LDL‐induced dysfunction via upregulating the expression of the miR‐22‐3p target genes CXCR2 and AKT

    DOI: 10.1016/j.febslet.2015.08.046

    Sample: Cell culture supernatant
  45. LIFE SCIENCES (2018) IF: 3.234
    LncRNA PMS2L2 protects ATDC5 chondrocytes against lipopolysaccharide-induced inflammatory injury by sponging miR-203

    DOI: 10.1016/j.lfs.2018.12.020

    PMID: 30550887

    Sample: Cell culture supernatant
  46. PLoS One (2022) IF: 3.24
    Clinical characteristics and outcomes of COVID-19 patients in Bali, Indonesia

    DOI: 10.1371/journal.pone.0269026

    PMID: 35687545

    Sample: serum
  47. Bioengineered (2022) IF: 3.269
    Nintedanib ameliorates oxidized low-density lipoprotein -induced inflammation and cellular senescence in vascular endothelial cells

    DOI: 10.1080/21655979.2022.2036913

    PMID: 35236245

    Sample: HUVECs
  48. Bioengineered (2021) IF: 3.269
    Apremilast mitigates interleukin (IL)-13-induced inflammatory response and mucin production in human nasal epithelial cells (hNECs)

    DOI: 10.1080/21655979.2021.1987818

    PMID: 34607526

    Sample: Human nasal epithelial cells (hNECs)
  49. Food & Function (2018) IF: 3.289
    Probiotic attributes and prevention of LPS-induced pro-inflammatory stress in RAW264.7 macrophages and human intestinal epithelial cell line (Caco-2) by newly isolated Weissella cibaria strains

    DOI: 10.1039/C7FO00469A

    Sample: Caco-2 cells
  50. CELLULAR IMMUNOLOGY (2019) IF: 3.291
    DosR antigen Rv1737c induces activation of macrophages dependent on the TLR2 pathway

    DOI: 10.1016/j.cellimm.2019.103947

    Sample: THP-1 cells
  51. JOURNAL OF CELLULAR BIOCHEMISTRY (2019) IF: 3.448
    Eriodictyol inhibits survival and inflammatory responses and promotes apoptosis in rheumatoid arthritis fibroblast‐like synoviocytes through AKT/FOXO1 signaling

    DOI: 10.1002/jcb.28724

    Sample: rheumatoid arthritis (RA) fibroblast-like synovioc
  52. JOURNAL OF NEUROIMMUNOLOGY (2022) IF: 3.478
    Association between pro-inflammatory cytokines in cerebrospinal fluid and headache in patients with aneurysmal subarachnoid hemorrhage

    DOI: 10.1016/j.jneuroim.2022.577841

    PMID: 35279426

    Sample: cerebrospinal fluid (CSF)
  53. ACS Omega (2021) IF: 3.512
    Roflumilast Reduced the IL-18-Induced Inflammatory Response in Fibroblast-Like Synoviocytes (FLS)

    DOI: 10.1021/acsomega.0c05281

    PMID: 33521454

    Sample: Cell culture supernatant
  54. Nanoscale Research Letters (2020) IF: 3.578
    Nec-1 Attenuates Neurotoxicity Induced by Titanium Dioxide Nanomaterials on Sh-Sy5y Cells Through RIP1

    DOI: 10.1186/s11671-020-03300-5

    Sample: SH-SY5Y cell
  55. TOXICOLOGY LETTERS (2017) IF: 3.858
    MicroRNA-218 acts by repressing TNFR1-mediated activation of NF-κB, which is involved in MUC5AC hyper-production and inflammation in smoking-induced bronchiolitis of COPD

    DOI: 10.1016/j.toxlet.2017.08.079

    Sample: Serum,Cell culture supernatant
  56. Annals of Translational Medicine (2021) IF: 3.932
    The liver X receptor agonist T0901317 reduces the inflammation of alveolar epithelial cells induced by polyhexamethylene guanidine through inhibition of the NFκB signaling pathway

    DOI: 10.21037/atm-21-6501

    Sample: A549 cell
  57. Annals of Translational Medicine (2022) IF: 3.932
    Advanced platelet-rich fibrin promotes the paracrine function and proliferation of adipose-derived stem cells and contributes to micro-autologous fat transplantation by modulating HIF-1α and VEGF

    DOI: 10.21037/atm-21-6812

    Sample: adipose-derived stem cell (ASCs)
  58. INTERNATIONAL IMMUNOPHARMACOLOGY (2020) IF: 3.943
    Mammalian Ste20-like kinase 4 inhibits the inflammatory response in Aspergillus fumigatus keratitis

    DOI: 10.1016/j.intimp.2020.107021

    PMID: 33182037

    Sample: Cell lysate
  59. NEUROGASTROENTEROLOGY AND MOTILITY (2022) IF: 3.96
    Somatization is associated with altered serum levels of vitamin D, serotonin, and brain-derived neurotrophic factor in patients with predominant diarrhea irritable bowel syndrome

    DOI: 10.1111/nmo.14512

    Sample: serum
  60. Scientific Reports (2020) IF: 3.998
    Validation of Salivary Markers, IL-1β, IL-8 and Lgals3bp for Detection of Oral Squamous Cell Carcinoma in an Indian Population

    DOI: 10.1038/s41598-020-64494-3

    Sample: Saliva
  61. Innate Immunity (2019) IF: 2.173
    Functional characterisation of Holothuria leucospilota Fas-associated death domain in the innate immune-related signalling pathways:

    DOI: 10.1177/1753425919877680

    Sample: Cell culture supernatant
  62. INTERNATIONAL DAIRY JOURNAL (2018) IF: 2.201
    Glutathione biosynthesis is essential for antioxidant and anti-inflammatory effects of Streptococcus thermophilus

    DOI: 10.1016/j.idairyj.2018.08.012

    Sample: Cell culture supernatant
  63. BRAZILIAN JOURNAL OF MICROBIOLOGY (2022) IF: 2.214
    In vitro characterization of lactic acid bacterial strains isolated from fermented foods with anti-inflammatory and dipeptidyl peptidase-IV inhibition potential

    DOI: 10.1007/s42770-022-00872-5

    Sample: Caco-2 cell
  64. ACTA ODONTOLOGICA SCANDINAVICA (2019) IF: 2.331
    Leptin induces IL-6 and IL-8 expression through leptin receptor Ob-Rb in human dental pulp fibroblasts

    DOI: 10.1080/00016357.2018.1536280

    PMID: 30600742

    Sample: Cell culture supernatant
  65. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2017) IF: 2.341
    Particulate matter 2.5 regulates lipid synthesis and inflammatory cytokine production in human SZ95 sebocytes

    DOI: 10.3892/ijmm.2017.3109

    Sample: Cell culture supernatant
  66. IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY (2020) IF: 2.352
    A case of asthma with Behcet’s disease: successful treatment with omalizumab and its effects on recurrent aphthous lesions

    DOI: 10.1080/08923973.2020.1789656

    Sample: Serum
  67. Journal of Orthopaedic Surgery and Research (2022) IF: 2.359
    Circ_0043947 contributes to interleukin 1β-induced injury in chondrocytes by sponging miR-671-5p to up-regulate RTN3 expression in osteoarthritis pathology

    DOI: 10.1186/s13018-022-02970-4

    PMID: 35331286

    Sample: chondrocytes
  68. MEDICAL MYCOLOGY (2017) IF: 2.377
    A fucose specific lectin from Aspergillus flavus induced interleukin-8 expression is mediated by mitogen activated protein kinase p38

    DOI: 10.1093/mmy/myw066

    Sample: Human L-132 cell,human monocyte cell line (U937),P
  69. CLINICAL RHEUMATOLOGY (2020) IF: 2.394
    The clinical value of interleukins-8, -10, and -17 in idiopathic granulomatous mastitis

    DOI: 10.1007/s10067-020-04925-8

    Sample: blood
  70. Experimental and Therapeutic Medicine (2021) IF: 2.447
    Mediators of extracellular matrix degradation and inflammation: A new team of possible biomarkers for oral squamous cell carcinoma stage

    DOI: 10.3892/etm.2021.10309

    PMID: 34194555

    Sample: Oral cancer tissue
  71. ACTA TROPICA (2018) IF: 2.509
    Assessment of chemokine and cytokine signatures in patients with dengue infection: A hospital-based study in Kolkata, India

    DOI: 10.1016/j.actatropica.2018.10.017

    PMID: 30395811

    Sample: Serum
  72. BIOSCIENCE REPORTS (2019) IF: 2.535
    Association of IL-6, IL-8, MMP-13 gene polymorphisms with knee osteoarthritis susceptibility in the Chinese Han population

    DOI: 10.1042/BSR20181346

    PMID: 30635366

    Sample: Tissue homogenate
  73. Clinical Respiratory Journal (2021) IF: 2.57
    Adhesion molecules as diagnostic and severity biomarkers in pediatric community-acquired pneumonia

    DOI: 10.1111/crj.13334

    PMID: 33484111

    Sample: Whole Blood,Serum
  74. MICROBIAL PATHOGENESIS (2019) IF: 2.581
    Isolation, characterization and analysis of pro-inflammatory potential of Klebsiella pneumoniae outer membrane vesicles

    DOI: 10.1016/j.micpath.2019.103719

    PMID: 31493501

    Sample: Cell culture supernatant
  75. Annals of Palliative Medicine (2021) IF: 2.595
    The effects of blood purification combined with antibiotics on extravascular lung water index, inflammatory factors, and prognosis of patients with severe acute pancreatitis complicated with acute respiratory distress syndrome.

    DOI: 10.21037/apm-21-2168

    Sample: blood
  76. Annals of Palliative Medicine (2021) IF: 2.595
    Effect of probiotics supplementation combined with WeChat platform health management on nutritional status, inflammatory factors, and quality of life in patients with mild-to-moderate ulcerative colitis: a randomized trial.

    DOI: 10.21037/apm-21-1056

    Sample: serum
  77. Dose-Response (2022) IF: 2.623
    Immune Cell and Biochemical Biomarkers in Advanced Laryngeal Cancer:

    DOI: 10.1177/15593258221115537

    Sample: serum
  78. Evidence-based Complementary and Alternative Medicine (2022) IF: 2.65
    Febuxostat Alleviates Allergic Rhinitis by Inhibiting Inflammation and Monocyte Adhesion in Human Nasal Epithelial Cells via Regulating KLF6.

    DOI: 10.1155/2022/9092311

    Sample: Human nasal epithelial cell (hNECs)
  79. ONCOLOGY REPORTS (2017) IF: 2.662
    PLD4 promotes M1 macrophages to perform antitumor effects in colon cancer cells

    DOI: 10.3892/or.2016.5216

    Sample: macrophage
  80. Food & Function (2016) IF: 2.686
    Antidiabetic (type 2) effects of Lactobacillus G15 and Q14 in rats through regulation of intestinal permeability and microbiota

    DOI: 10.1039/C6FO00831C

    Sample: Plasma,Serum
  81. EUROPEAN JOURNAL OF DERMATOLOGY (2022) IF: 2.805
    Role of the long non-coding RNA, SPRR2C, based on an in vitro psoriatic keratinocyte cell model

    DOI: 10.1684/ejd.2022.4247

    PMID: 35866915

    Sample: Ker-CT cell,HaCaT cell
  82. HUMAN & EXPERIMENTAL TOXICOLOGY (2022) IF: 2.903
    Fluorometholone inhibits high glucose-induced cellular senescence in human retinal endothelial cells:

    DOI: 10.1177/09603271221076107

    Sample: human retinal endothelial cell
  83. MICROBIAL PATHOGENESIS (2020) IF: 2.914
    Curcumin ameliorates Staphylococcus aureus-induced mastitis injury through attenuating TLR2-mediated NF−κB activation

    DOI: 10.1016/j.micpath.2020.104054

    Sample: Serum
  84. Oncology Letters (2021) IF: 2.967
    Association between the serum concentrations and mutational status of IL‑8, IL‑27 and VEGF and the expression levels of the hERG potassium channel gene in patients with colorectal cancer

    DOI: 10.3892/ol.2021.12926

    Sample: serum
  85. TRANSPLANT IMMUNOLOGY (2021) IF: 1.708
    miR-1287-5p upregulation inhibits the EMT and pro-inflammatory cytokines in LPS-induced human nasal epithelial cells (HNECs)

    DOI: 10.1016/j.trim.2021.101429

    PMID: 34139308

    Sample: HNECs cells
  86. ARCHIVES OF ORAL BIOLOGY (2020) IF: 1.931
    Nec-1 attenuates inflammation and cytotoxicity induced by high glucose on THP-1 derived macrophages through RIP1

    DOI: 10.1016/j.archoralbio.2020.104858

    PMID: 32805637

    Sample: Cell culture supernatant
  87. ACTA CLINICA BELGICA (2019) IF: 0.96
    Interleukin 8 as predictive factor for response to chemotherapy in colorectal cancer patients

    DOI: 10.1080/17843286.2019.1680133

    Sample: serum
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