IMDM(Iscove’s Modified Dulbecco Medium)is an improved DMEM medium， which is used for the culture of erythrocyte progenitor cells and macrophages. The IMDM medium was supplemented with selenium, HEPES, sodium pyruvate and additional amino acids and vitamins on the basis of the DMEM medium, and potassium nitrate instead of iron nitrate, which is suitable for the rapid proliferation, high-density cell culture. IMDM medium can not only culture cells with special nutritional requirements (such as mouse B lymphocytes, stimulated B cells stimulated by LPS, bone marrow hematopoietic cells, T lymphocytes and various hybridoma cells), It can also be used as a base solution for some unique serum-free medium. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

General Information

Product Form	Powder
pH	7.2-7.4
D-Glucose	4500mg/L
HEPES	25mM
L-glutamine	4mM
Phenol red	15mg/L
Sodium pyruvate	1 mM
Storage	2-8℃ ,Shading Light
Shipping	RT
Expiration date	36 months

Notes

1. This product is for research use only.;
2. It is necessary to pay attention to the aseptic operation and avoid the contamination.

Technical Resources

Recommendations for handling of cryopreserved cells

Protocol for Thawing Frozen Cells

Count the number of cells with Trypan Blue exclusion

Citations

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