Background
L-alanyl-L-glutamine is an advanced cell culture additive and as a substitute for L-glutamine. L-glutamine is a necessary nutrient element for cells. But it is unstable in solution and can be degraded spontaneously to form ammonia and pyroglutamic acid and ammonia is harmful to cells. L-alanyl-L-glutamine is stable in aqueous solution and does not degrade spontaneously. The mechanism used by the cell is that the cell gradually releases a peptidase into the culture medium during cell culture. The peptidase can hydrolyze L-alanyl-L-glutamine into L-alanine and L-glutamine. L-alanine and L-glutamine can be absorbed and utilized by cells. This not only increases the utilization rate of L-glutamine, but also does not produce excess ammonia, which is more beneficial to cell growth.
General Information
| Product Form |
Liquid |
| Concentration |
200mM |
| L-Alanyl-L-glutamine |
200mM |
| Recommended working concentration |
1mM-4mM |
| Dissolvent |
0.85% NaCl |
| Storage |
2-8℃ |
| Shipping |
RT |
| Expiration date |
36 months |
Notes
1. This product is for research use only.;
2. This product is sterilized by 0.1μm filtration.;
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.;
4. It will take some time to release peptidase and metabolize dipeptide, so the delay period of cells will be slightly longer.; But there is no effect to cell growth.;
5. This product is a concentrated solution and dilute it to the desired concentration before use.
Citations
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