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Lipid Peroxide (LPO) Colorimetric Assay Kit

Cat.No.:E-BC-K176-M
Detection instrument: Microplate reader(580-590 nm)

Detection method: Colorimetric method

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Detection principle

With 45℃ incubation for 60 min, one molecule of LPO react with two molecule of chromogenic reagent, to produce a stable chromophore which have the maximum absorption peak at 586nm. The content of LPO in samples can be calculated by standard curve or calculation formula.

Performance characteristics

Synonyms	LPO
Sample type	Serum,plasma,urine,tissue
Sensitivity	0.70 μmol/L
Detection range	0.70-80 μmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	90 min
Precision	Average inter-assay CV: 3.500%Average intra-assay CV: 3.100%
Other instruments required	Water bath, Centrifuge, Micropipettor, Vortex mixer
Other reagents required	Double distilled water, PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Images

LPO level was induced significantly after treated with H2O2 of 50 μM. (*P<0.05)

A V Shchulkin et al investigate the function of P-glycoprotein in decreasing cell membranes permeability during oxidative stress. Lipid peroxide (LPO) level in human cell was determined using LPO colorimetric assay kit (E-BC-K176-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range ( 0.70-80 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Human plasma	1
Urine	1
10% Rat kidney tissue homogenization	1
10% Rat liver tissue homogenization	1

Note: The diluent is PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Frontiers in Pharmacology (2021) IF: 5.811

The Anti-Inflammatory Effect of a Combination of Five Compounds From Five Chinese Herbal Medicines Used in the Treatment of COPD

DOI: 10.3389/fphar.2021.709702
	Sample: serum

BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545

Vitamin E and selenium administration synergistically mitigates ivermectin and doramectin-induced testicular dysfunction in male Wistar albino rats

DOI: 10.1016/j.biopha.2020.109841
PMID: 31972360	Sample: Tissue homogenate

Biomed Research International (2021) IF: 3.411

Antioxidant Activity Derived from Marine Green-Lipped Mussel Perna canaliculus Extracts in Mice

DOI: 10.1155/2021/1622270
PMID: 34409099	Sample: liver,kidney,cardiac

International Journal of Endocrinology (2020) IF: 2.299

Effect of Light-Dark Cycle Misalignment on the Hypothalamic-Pituitary-Gonadal Axis, Testicular Oxidative Stress, and Expression of Clock Genes in Adult Male Rats

DOI: 10.1155/2020/1426846
PMID: 33204259	Sample: Tissue homogenate(Testicular)

BIOCHEMISTRY-MOSCOW (2021) IF: 2.487

The Role of P-Glycoprotein in Decreasing Cell Membranes Permeability during Oxidative Stress

DOI: 10.1134/S0006297921020085
PMID: 33832418	Sample: Cell（Human colon adenocarci noma (Caco-2) cells

JOURNAL OF FOOD BIOCHEMISTRY (2021) IF: 2.72

Antioxidant and antithrombotic effects of green mussels (Perna canaliculus) in rats

DOI: 10.1111/jfbc.13865
PMID: 34263474	Sample: Liver，kidney

International Journal of Chronic Obstructive Pulmonary Disease (2022) IF: 2.893

Myostatin/HIF2α-Mediated Ferroptosis is Involved in Skeletal Muscle Dysfunction in Chronic Obstructive Pulmonary Disease

DOI: 10.2147/COPD.S377226
	Sample: skeletal muscle,C2C12 myotube

ANDROLOGIA (2020) IF: 1.951

Androgenic effect of aqueous leaf extract of Moringa oleifera on Leydig TM3 cells in vitro

DOI: 10.1111/and.13825
PMID: 32926461	Sample: Cell lysate

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Reviews/Q&A

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Reviews
Q&A

M**********dSubmitted [ Aug 18 2022 ]

Asked: Is LPO used as a biomarker for cell apoptosis?

adminSubmitted [ Aug 18 2022 ]

Answered:

D********dSubmitted [ Aug 02 2022 ]

Asked: How long can I store the tissue homogenization? Any requirement?

adminSubmitted [ Aug 02 2022 ]

Answered: It's better to use the tissue homogenization immediately after prepared. But you can store the tissue sample at -80 degree for no more than 1 month.

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