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Cat.No.:E-BC-K176-M
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Synonyms | LPO |
Sample type | Serum,plasma,urine,tissue |
Sensitivity | 0.70 μmol/L |
Detection range | 0.70-80 μmol/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 90 min |
Precision | Average inter-assay CV: 3.500%Average intra-assay CV: 3.100% |
Other instruments required | Water bath, Centrifuge, Micropipettor, Vortex mixer |
Other reagents required | Double distilled water, PBS (0.01 M, pH 7.4) |
Storage | 2-8℃ |
Valid period | 12 months |
LPO level was induced significantly after treated with H2O2 of 50 μM. (*P<0.05)
A V Shchulkin et al investigate the function of P-glycoprotein in decreasing cell membranes permeability during oxidative stress. Lipid peroxide (LPO) level in human cell was determined using LPO colorimetric assay kit (E-BC-K176-M).
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range ( 0.70-80 μmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Human serum |
1 |
Human plasma |
1 |
Urine |
1 |
10% Rat kidney tissue homogenization |
1 |
10% Rat liver tissue homogenization |
1 |
Note: The diluent is PBS (0.01 M, pH 7.4).
Sample: serum |
PMID: 31972360 |
Sample: Tissue homogenate |
DOI: 10.1155/2021/1622270 |
|
PMID: 34409099 |
Sample: liver,kidney,cardiac |
DOI: 10.1155/2020/1426846 |
|
PMID: 33204259 |
Sample: Tissue homogenate(Testicular) |
PMID: 33832418 |
Sample: Cell(Human colon adenocarci noma (Caco-2) cells |
DOI: 10.1111/jfbc.13865 |
|
PMID: 34263474 |
Sample: Liver,kidney |
DOI: 10.2147/COPD.S377226 |
|
Sample: skeletal muscle,C2C12 myotube |
DOI: 10.1111/and.13825 |
|
PMID: 32926461 |
Sample: Cell lysate |
M**********dSubmitted [ Aug 18 2022 ]
Asked: Is LPO used as a biomarker for cell apoptosis?
Reply
adminSubmitted [ Aug 18 2022 ]
Answered:
D********dSubmitted [ Aug 02 2022 ]
Asked: How long can I store the tissue homogenization? Any requirement?
Reply
adminSubmitted [ Aug 02 2022 ]
Answered: It's better to use the tissue homogenization immediately after prepared. But you can store the tissue sample at -80 degree for no more than 1 month.