LLC-MK2 cell line


      Catalog number:EP-CL-0141

      • 1×10⁶ cells/vial
      - +
      Price: $347

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      Origin and General Characteristics

      Cell Name LLC-MK2 cell line
      Organism Macaca mulatta, Monkey, Rhesus
      Age adult
      Tissue Kidney
      Morphology Epithelial
      Growth Properties Adherent
      Descriptions This cell line was established in the 1950s from a renal tissue pooled suspension from six rhesus monkeys (Macaca mulatta). The cells are reported susceptible to a wide range of viruses including members of the enterovirus, rhinovirus, mucinous and poxvirus groups.
      Biosafety Level 1

      Culture Conditions and Handling

      Complete Growth Medium RPMI-1640+10% FBS+1% P/S
      Subculturing Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
      Subcultivation Ratio 1: 3-1: 4
      Medium Renewal Every 2 to 3 days
      Cryopreservation Freeze medium: 60% Basal medium+30% FBS+10% DMSO
      Storage temperature: Liquid nitrogen vapor phase
      Culture Conditions Atmosphere: Air, 95%; CO2, 5%
      Temperature: 37℃

      Special Features of the Cell Line

      Gene Expression Plasminogen activator

      Related Resources

      Recommendations for handling of cryopreserved cells

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