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Mouse IL-2(Interleukin 2) ELISA Kit

Citations(36)Uniprot : P04351

  • Cat.No.:E-EL-M0042

  • Reactivity: Mouse
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Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse IL-2 in samples. No significant cross-reactivity or interference between Mouse IL-2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse IL-2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-2. You can calculate the concentration of Mouse IL-2 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IL-2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IL-2 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 49.00 87.40 385.60 52.40 82.20 365.00
Standard deviation 3.30 3.80 20.40 3.10 4.80 15.70
CV (%) 6.73 4.35 5.29 5.92 5.84 4.30

Recovery

The recovery of Mouse IL-2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-101 93
EDTA plasma (n=8) 88-103 94
Cell culture media (n=8) 89-102 96

Linearity

Samples were spiked with high concentrations of Mouse IL-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P04351
SynonymsIL2, TCGF, Lymphokine
Research AreaCancer, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Science Translational Medicine (2020) IF: 16.304
    Combination of metabolic intervention and T cell therapy enhances solid tumor immunotherapy

    DOI: 10.1126/scitranslmed.aaz6667

    PMID: 33239389

    		 Sample: Cell culture supernatant
  2. Biomaterials Research (2022) IF: 15.863
    Highly tailorable gellan gum nanoparticles as a platform for the development of T cell activator systems

    DOI: 10.1186/s40824-022-00297-z

    PMID: 36180901

    		 Sample: splenocytes
  3. Science Advances (2022) IF: 14.957
    Glucocorticoid-induced loss of beneficial gut bacterial extracellular vesicles is associated with the pathogenesis of osteonecrosis

    DOI: 10.1126/sciadv.abg8335
    Sample: serum
  4. Materials Today Bio (2023) IF: 10.761
    Treg-targeted efficient-inducible platform for collagen-induced arthritis treatment

    DOI: 10.1016/j.mtbio.2023.100557
    Sample: Lymphocytes
  5. CARBOHYDRATE POLYMERS (2021) IF: 9.381
    Dissolvable microneedles based on Panax notoginseng polysaccharide for transdermal drug delivery and skin dendritic cell activation

    DOI: 10.1016/j.carbpol.2021.118211

    PMID: 34127215

    		 Sample: Tissue Slice
  6. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022) IF: 8.025
    Structural characterization of a soluble polysaccharide SSPS1 from soy whey and its immunoregulatory activity in macrophages

    DOI: 10.1016/j.ijbiomac.2022.07.043

    PMID: 35835298

    		 Sample: Raw 264.7 macrophages
  7. Antioxidants (2021) IF: 6.313
    Aronia melanocarpa Extract Fermented by Lactobacillus plantarum EJ2014 Modulates Immune Response in Mice

    DOI: 10.3390/antiox10081276

    PMID: 34439524

    		 Sample: plasma
  8. JOURNAL OF ETHNOPHARMACOLOGY (2023) IF: 5.195
    Immunomodulatory effects and mechanisms of Tiepishihu Xiyangshen granules on cyclophosphamide induced immuno-suppression via TLR4/MAPKs and PI3K/AKT/FOXO3a signal pathways

    DOI: 10.1016/j.jep.2023.116192
    Sample: serum
  9. INTERNATIONAL JOURNAL OF PHARMACEUTICS (2022) IF: 5.875
    A different approach to immunochemotherapy for colon Cancer: Development of nanoplexes of cyclodextrins and Interleukin-2 loaded with 5-FU

    DOI: 10.1016/j.ijpharm.2022.121940

    PMID: 35724824

    		 Sample: CD nanoplexes
  10. PHYTOTHERAPY RESEARCH (2021) IF: 5.882
    Erianin inhibits human lung cancer cell growth via PI3K/Akt/mTOR pathway in vitro and in vivo

    DOI: 10.1002/ptr.7154

    PMID: 34236105

    		 Sample: LLC cell-Xenograft
  11. AAPS PHARMSCITECH (2022) IF: 4.026
    Antitumor Immunotherapy of Sialic Acid and/or GM1 Modified Coenzyme Q10 Submicron Emulsion

    DOI: 10.1208/s12249-022-02426-2

    PMID: 36253573

    		 Sample: serum
  12. CARBOHYDRATE POLYMERS (2015) IF: 4.074
    Structural analysis and immunoregulation activity comparison of five polysaccharides from Angelica sinensis

    DOI: 10.1016/j.carbpol.2015.12.050
    Sample: Cell culture supernatant
  13. Frontiers in Microbiology (2020) IF: 4.236
    Immunization With a Secreted Esterase Protects Mice Against Multiple Serotypes (M1, M3, and M28) of Group A Streptococcus

    DOI: 10.3389/fmicb.2020.00565
    Sample: skin
  14. Scientific Reports (2017) IF: 4.259
    Rheb1 deletion in myeloid cells aggravates OVA-induced allergic inflammation in mice

    DOI: 10.1038/srep42655
    Sample: Bronchoalveolar Lavage Fluid,Serum
  15. BIOLOGY OF REPRODUCTION (2022) IF: 4.285
    A decrease in cluster of differentiation 2 expression on natural killer cells is associated with polycystic ovary syndrome but not influenced by metformin in a mouse model

    DOI: 10.1093/biolre/ioac004
    Sample: serum
  16. JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
    Danggui Buxue Decoction enhances the anticancer activity of gemcitabine and alleviates gemcitabine-induced myelosuppression

    DOI: 10.1016/j.jep.2021.113965

    PMID: 33639205

    		 Sample: Serum,Tissue(spleen)
  17. JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION (2021) IF: 4.399
    Attenuation of virulence in multiple serotypes (M1, M3, and M28) of Group A Streptococcus after the loss of secreted esterase

    DOI: 10.1016/j.jmii.2021.09.008

    PMID: 34674958

    		 Sample: skin lesion
  18. INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932
    The immunomodulatory effects of ginsenoside derivative Rh2-O on splenic lymphocytes in H22 tumor-bearing mice is partially mediated by TLR4

    DOI: 10.1016/j.intimp.2021.108316

    PMID: 34768129

    		 Sample: splenic lymphocytes
  19. Journal of Functional Foods (2017) IF: 3.144
    Antitumor and immunomodulatory effects of ginsenoside Rh2 and its octyl ester derivative in H22 tumor-bearing mice

    DOI: 10.1016/j.jff.2017.03.013
    Sample: Serum
  20. Food & Function (2018) IF: 3.289
    Structural characterization of two endopolysaccharides from Phellinus sp. and their immunologic effects by intragastric administration in a healthy mammalian model

    DOI: 10.1039/C7FO01161J
    Sample: serum
  21. VETERINARY MICROBIOLOGY (2021) IF: 3.293
    Molecular and functional characterization of HtrA protein in Actinobacillus pleuropneumoniae

    DOI: 10.1016/j.vetmic.2021.109058

    PMID: 33862332

    		 Sample: Serum
  22. INTERNATIONAL IMMUNOPHARMACOLOGY (2019) IF: 3.361
    Fusion of thymosin alpha 1 with mutant IgG1 CH3 prolongs half-life and enhances antitumor effects in vivo

    DOI: 10.1016/j.intimp.2019.05.047

    PMID: 31220695

    		 Sample: Serum
  23. GENE (2016) IF: 3.688
    Immunogenicity of the recombinant HxuCBA proteins encoded by hxuCBA gene cluster of Haemophilus parasuis in mice

    DOI: 10.1016/j.gene.2016.07.001
    Sample: Serum
  24. Parasites & Vectors (2021) IF: 3.876
    In vivo and in vitro efficacy of crocin against Echinococcus multilocularis

    DOI: 10.1186/s13071-021-04866-4

    PMID: 34256821

    		 Sample: serum
  25. Parasites & Vectors (2021) IF: 3.876
    In vitro and in vivo efficacy of thiacloprid against Echinococcus multilocularis

    DOI: 10.1186/s13071-021-04952-7

    PMID: 34488852

    		 Sample: serum
  26. PARASITOLOGY RESEARCH (2022) IF: 2.383
    Plasmodium knowlesi Duffy binding protein alpha region II (PkDBPαII) in clinical isolates from Peninsular Malaysia and Malaysian Borneo exhibit different immune responses in animal models

    DOI: 10.1007/s00436-022-07665-7

    PMID: 36152079

    		 Sample: Spleen cell
  27. MICROBIAL PATHOGENESIS (2019) IF: 2.581
    Metabolites of stable fly reduce diarrhea in mice by modulating the immune system, antioxidants, and composition of gut microbiota

    DOI: 10.1016/j.micpath.2019.103557
    Sample: serum,small intestinal tissues
  28. PLoS One (2018) IF: 2.766
    A trivalent Apx-fusion protein delivered by E. coli outer membrane vesicles induce protection against Actinobacillus pleuropneumoniae of serotype 1 and 7 challenge in a murine model

    DOI: 10.1371/journal.pone.0191286
    Sample: serum
  29. MICROBIAL PATHOGENESIS (2020) IF: 2.914
    Protective immunity induced by a DNA vaccine cocktail expressing TgSAG1, TgROP2, and the genetic adjuvant HBsAg against Toxoplasma gondii infection

    DOI: 10.1016/j.micpath.2020.104441

    PMID: 32781100

    		 Sample: Serum,Cell culture supernatant
  30. MICROBIAL PATHOGENESIS (2020) IF: 2.914
    Curcumin ameliorates Staphylococcus aureus-induced mastitis injury through attenuating TLR2-mediated NF−κB activation

    DOI: 10.1016/j.micpath.2020.104054
    Sample: Serum
  31. Molecular Medicine Reports (2019) IF: 2.952
    BCG stimulation promotes dendritic cell proliferation and expression of VDR and CYP27B1 in vitamin D‑deficient mice

    DOI: 10.3892/mmr.2019.10780
    Sample: BMDCs
  32. ONCOLOGY REPORTS (2018) IF: 2.976
    Effect of xanthohumol on Th1/Th2 balance in a breast cancer mouse model

    DOI: 10.3892/or.2017.6094
    Sample: serum
  33. International Journal of Clinical and Experimental Medicine (2015) IF: 1.277
    Sulfated modification promotes the immunomodulatory bioactivities of Lyciumbarbarum polysaccharides in vitro

    PMID: 26884954

    		 Sample: lymphocyte
  34. ACTA PARASITOLOGICA (2021) IF: 1.44
    Evaluation of Allicin Against Alveolar Echinococcosis In Vitro and in a Mouse Model

    DOI: 10.1007/s11686-021-00434-z

    PMID: 34143400

    		 Sample: serum
  35. RESEARCH IN VETERINARY SCIENCE (2019) IF: 1.751
    Amelioration effects of Kaempferol on immune response following chronic intermittent cold-stress

    DOI: 10.1016/j.rvsc.2019.08.012

    PMID: 31412308

    		 Sample: Serum,Tissue homogenate
  36. JOURNAL OF VIROLOGICAL METHODS (2020) IF: 1.786
    Colonisation of mice and pigs by a chimeric porcine circovirus 1–2 prototype vaccine strain and a PCV2 isolate originating in China and their induction of cytokines

    DOI: 10.1016/j.jviromet.2020.113905
    Sample: Cell culture supernatant
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