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Ni Focurose 6FF(IDA)

  • Cat.No.:E-CM-AF08

To Purchase E-CM-AF08

Size:
  • 5mL prepacked column
  • 25mL
  • 100mL
  • 500mL
Price: $160
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Product Introduction

Ni Focurose 6FF (IDA) can be used for separation and purification with the interactions between Ni2+and some amino acids (mainly include histidine, cysteine, tryptophan) on the side chain of protein, and it is suitable for separation and purification of His-tagged protein and biological molecules which interact with Ni2+.

Product Features

1. Quick and easy (one-step purification).
2. Wide application, simple-operation. Suitable for gravity column and prepacked column.
3. Multiple selection. Other metal ions (e.g. Cu2+, Zn2+, Fe2+, Co2+, Ca2+, etc.) can be used if Ni2+ cannot be applied well.

Product Datasheet

Matrix Highly cross-linked 6% agarose
Particle size range 45-165 µm
Average particle size 90 µm
Binding capacity 40-50 mg (His-tagged protein)/mL (media)
pH stability 3-12(long-term),2-14(short term)
Chemical stability All of the commonly used solution. Avoid of chelating agent(e.g. EDTA, EGTA) and reductant(e.g. DTT, DTE).
Maximum flow rate 300-600 cm/h(0.3MPa, XK16/40. Column bed height:30 cm)
Pressure ≤ 0.3 MPa
Storage buffer 20% Ethanol
Storage temperature 4~8℃

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