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Ni Focurose 6FF(TED)

  • Cat.No.:E-CM-AF06

To Purchase E-CM-AF06

Size:
  • 5mL prepacked column
  • 25mL
  • 100mL
  • 500mL
Price: $160
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Product Introduction

Ni-6FF (TED) can be used for separation and purification with the interactions between Ni2+and some amino acids (mainly include histidine, cysteine, tryptophan) on the side chain of protein, and it is suitable for separation and purification of His-tagged protein and biological molecules which interact with Ni2+. The strong binding Ni2+ can be directly used for His-tagged protein expressed by eukaryotic expression system, and it have resistance for higher concentration of reductant and chelating agent. In addition, sample pretreatment is unnecessary. The cleaning and reviving of media are simple, which can be washed directly without nickel removal.

Product Features

1. Quick and easy (one-step purification).
2. Tolerate to higher concentration of reductant and chelating agent. His-tagged protein expressed by eukaryotic expression system can be loading without pretreatment, which can protect the protein activity to the utmost.
3. There is no need to remove nickel removal can wash with NaOH directly, which greatly shorten the cleaning period.
4. Lower Ni2+ abscission than Ni-6FF (IMAC) and Ni-6FF (IDA). No need of repeated revival.

Product Datasheet

Matrix Highly cross-linked 6% agarose
Particle size range 45-165 µm
Average particle size 90 µm
Binding capacity 20~30 mg(His-tagged protein)/mL(media)
Media type Affinity Chromatography Medium
pH stability 3-12(working)2-14(washing)
Chemical stability 0.01MHCl, 0.01M NaOH(1 week) 20 mM EDTA, 10 mM DTT, 1M NaOH, 8M Urea, 6M Guanidine hydrochloride(24 hours) 100 mM EDTA, 0.5M Iminazole(2 hours) 30% Isopropyl alcohol(20 minutes)
Maximum flow rate 150-600 cm/h (0.3MPa, XK16/40. Column bed height:30 cm)
Pressure ≤ 0.3 MPa
Storage buffer 20% Ethanol
Storage temperature 4~8℃

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