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Cat.No.:E-CM-AF06
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Matrix | Highly cross-linked 6% agarose |
Particle size range | 45-165 µm |
Average particle size | 90 µm |
Binding capacity | 20~30 mg(His-tagged protein)/mL(media) |
Media type | Affinity Chromatography Medium |
pH stability | 3-12(working)2-14(washing) |
Chemical stability | 0.01MHCl, 0.01M NaOH(1 week) 20 mM EDTA, 10 mM DTT, 1M NaOH, 8M Urea, 6M Guanidine hydrochloride(24 hours) 100 mM EDTA, 0.5M Iminazole(2 hours) 30% Isopropyl alcohol(20 minutes) |
Maximum flow rate | 150-600 cm/h (0.3MPa, XK16/40. Column bed height:30 cm) |
Pressure | ≤ 0.3 MPa |
Storage buffer | 20% Ethanol |
Storage temperature | 4~8℃ |