Octyl-4FF can interact with some hydrophobic protein or antibody under high ionic strength conditions (high ionic strength may increase the interaction between ligand and hydrophobic groups), thus to achieve the purpose of separation and purification. Octyl-4FF is mainly used for capture of initial samples and moderate and fine purification of samples.

Product Features

1. Rapid, easy to use (one-step purification).
2. Compared with reversed-phase chromatography, the ligand concentration in the hydrophobic interaction chromatography media is low and the elution conditions are mild, which helps to maintain the biological activity of biomolecules.
3. Wide application. Octyl-4FF can be applied in preliminary capture, moderate purification and fine purification, and it can be repeatedly used in combination ion exchange chromatography media.

Product Datasheet

Matrix	Highly cross-linked 4% agarose
Particle size range	45-65 µm
Average particle size	90 µm
Binding capacity	~10 mg(Lysyme)/mL(media)
Ligand	Octyl
Ligand density	~5 µmol/mL
Media type	Hydrophobic Interaction Medium
pH stability	2-14(short-term) 3-13(long-term)
Chemical stability	Chemical stability All of the commonly used buffers, 1M Acetic acid, 1M NaOH, 8M urea, 6M guanidine hydrochloride, 30% Isopropyl alcohol, 70% Ethanol
Maximum flow rate	300-700 cm/h (0.3 MPa, XK16/40, column bed height: 30 cm)
Storage buffer	20% Ethanol
Storage temperature	4~30℃(4~8℃ is preferred)

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Cat. No	E-CM-HI07
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