Balanced Salt Solution (Physiological Solution) have the properties of buffer solution (regulate pH), normal saline (maintain osmotic pressure) and culture medium (provide nutrition). It can meet the basic needs of survival and metabolism of tissues, organs or cells in vitro. A small amount of phenolic red was added to some equilibrium salt solutions to indicate the pH change of the solution. Phosphate Buffered Saline (PBS) is one of the most widely used equilibrium salt solutions in biochemistry. The main components are Na2HPO4、KH2PO4、NaCl and KCl. PBS buffers are often used to rinse tissue or cells, to transport cells or tissues, to prepare other reagents, to dilute the cells when counting the cells.

General Information

Product Form	Liquid
Concentration	100mM
pH	7.2-7.4
NaHCO₃	negative
Phenol red	negative
Ca2+-Mg2+	Negative
Storage	2-30℃
Shipping	RT
Expiration date	36 months

Notes

1. This product is for research use only.;
2. This product is sterilized by 0.1μm filtration.;
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
4. This product is a concentrated solution and dilute it to the desired concentration before use.

Technical Resources

Recommendations for handling of cryopreserved cells

Protocol for Thawing Frozen Cells

Count the number of cells with Trypan Blue exclusion

Citations

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