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Plant Flavonoids Colorimetric Assay Kit

  • Cat.No.:E-BC-K284-S

  • Detection instrument: Spectrophotometer(510 nm)

To Purchase E-BC-K284-S

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  • 100Assays
  • 50Assays
Price: $260
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For research use only. Order now, ship in 3 days

Detection principle

In alkaline nitrite solution, flavonoids form red complex with aluminum ion. The flavonoid content of the sample can be calculated by measuring the absorptivity of the sample extract at 510 nm.

Performance characteristics

Sample type Plant tissue
Sensitivity 0.315 μg/mL
Detection range 0.315-150 μg/mL
Detection method Colorimetric method
Assay type Quantitative
Assay time 60 min
Precision Average inter-assay CV: 2.200%Average intra-assay CV: 1.900%
Other instruments required Vortex mixer, Micropipettor, Air oven , Crusher, Ultrasonic cell processor
Other reagents required 60% Ethanol
Storage 2-8℃
Valid period 12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.315-150 μg/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Camphor leaves tissue homogenization

8-15

Carrot tissue homogenization

2-5

 Green pepper tissue homogenization

1

Note: The diluent is 60% Ethanol.

Citations

  1. Antioxidants (2022) IF: 6.313
    Phytochemical Characterization of Taxus baccata L. Aril with Emphasis on Evaluation of the Antiproliferative and Pro-Apoptotic Activity of Rhodoxanthin

    DOI: 10.3390/antiox11061039

    PMID: 35739936

    Sample: aril
  2. Nutrients (2022) IF: 6.706
    Grifola frondosa Extract Containing Bioactive Components Blocks Skin Fibroblastic Inflammation and Cytotoxicity Caused by Endocrine Disrupting Chemical, Bisphenol A

    DOI: 10.3390/nu14183812

    PMID: 36145189

    Sample: fruit
  3. INDUSTRIAL CROPS AND PRODUCTS (2021) IF: 5.645
    Bruceine D, the main active ingredient of Brucea javanica (L.) residue inhibits the germination of Bidens pilosa L. seeds by suppressing phenylpropanoid biosynthesis

    DOI: 10.1016/j.indcrop.2021.114079

    Sample: seedling
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Reviews/Q&A

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  • Reviews
  • Q&A
Q

M**********dSubmitted [ Aug 12 2022 ]

Asked: How long does it take for me to get the kit after ordering?

Reply

A

adminSubmitted [ Aug 12 2022 ]

Answered: It takes about 2 days preparing the kit, and about 2 weeks for the shipment based on the situation of express delivery.

Q

D********dSubmitted [ Aug 01 2022 ]

Asked: Is it possible to use the kit with in 96-wells plate instead of cuvette?

Reply

A

adminSubmitted [ Aug 01 2022 ]

Answered: I'll never recommend you to do so. But there is E-BC-K284-M which is designed for 96-well plate specifically.

Q

C*************oSubmitted [ Nov 24 2021 ]

Asked: Is it possible to perform the assay in a 96 wells microplate after reducing the volumes? Thank you

Reply

A

adminSubmitted [ Nov 24 2021 ]

Answered: Dear Chiara Thanks for your message. It is not recommended to reduce the sample volume. If possible, you can choose to dilute the sample to achieve the required sample volume. Best regards

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