Current Position:Home>>Kits>>Metabolism Assays>> Proline (Pro) Colorimetric Assay Kit

Proline (Pro) Colorimetric Assay Kit

Cat.No.:E-BC-K177-S
Detection instrument: Spectrophotometer(520 nm)

Detection method: Colorimetric method

To Purchase E-BC-K177-S

Size:

100Assays
50Assays

Price: $360

Qty:

Welcome to order from local distributors.

Protocols for metabolism assays

FAQ for metabolism assays

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Detection principle

Proline can react with acidic-ninhydrin to form stable red compound. The maximum absorption peak of the compound is at 520 nm. And the concentration of Pro can be calculated by measuring the OD value at 520 nm.

Performance characteristics

Synonyms	Pro
Sample type	Plant tissue,honey
Sensitivity	0.17 μg/mL
Detection range	0.17-35 μg/mL
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	80 min
Precision	Average inter-assay CV: 5.400%Average intra-assay CV: 4.500%
Other instruments required	Water bath, Micropipettor, Vortex mixer, Balance, Centrifuge
Other reagents required	Acetic acid, Methylbenzene, Absolute ethyl alcohol
Storage	2-8℃
Valid period	12 months

Images

The levels of proline concentration was significantly increased with salt tolerance and reversed by WR10. (**P<0.01 vs Control, ##P<0.01 vs NaCl)

ZH Yue et al investigate the function of Bacillus altitudinis for improving salt tolerance in wheat. Proline (Pro) level in wheat tissue was determined using Proline colorimetric assay kit (E-BC-K177-S).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.17-35 μg/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Green pepper tissue homogenization	1
Carrot tissue homogenization	1
Epipremnum aureum tissue homogenization	1
Lettuce leaf tissue homogenization	1
Honey	1
Cabbages tissue homogenization	1

Note: The diluent is Reagent 1.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Frontiers in Plant Science (2022) IF: 6.627

Halotolerant Bacillus altitudinis WR10 improves salt tolerance in wheat via a multi-level mechanism

DOI: 10.3389/fpls.2022.941388
PMID: 35909740	Sample: shoot

Reviews/Q&A

Show all
Reviews
Q&A

D*********aSubmitted [ Nov 28 2022 ]

Asked: as asked previously above, in your Application sheet you don\'t indicate the concentrations of methylbenzene and acetic acid preparations, which WE have to prepare, as they are not provided in the kit. Without knowing such concentrations, it is not posible to perform the assays. Culd you please provide the needed concentrations? Thank you.

adminSubmitted [ Nov 28 2022 ]

Answered: Dear Georgina, we will reply to you by email. Please pay attention to the mailbox

M**********dSubmitted [ Aug 18 2022 ]

Asked: Any other solvent can replace toluene?

adminSubmitted [ Aug 18 2022 ]

Answered:

D********dSubmitted [ Aug 02 2022 ]

Asked: Is it necessary to fit a standard curve for calculation?

adminSubmitted [ Aug 02 2022 ]

Answered: You don't have to. The linearity is good enough to calculated with a standard sample, and fitting a standard curve will take you a lot of time. But the situation only happens for this kit.

A***************iSubmitted [ Jan 09 2019 ]

Asked: Hi, I was wondering whether this kit is suitable for measuring proline in animal tissue or mammalian cells. Thanks Alessandro

adminSubmitted [ Jan 09 2019 ]

Answered: Dear Alessandro For this kit, we only tested on plant samples, for your samples, we advise to do a pre-test.

v*ySubmitted [ Oct 12 2018 ]

Asked: Hello, we have receive your Proline Assy Kit. We would likte to process a quantification but in your Application sheet you don\'t indicate the concentrations of methylbenzene and acetic acid preparations. Could you give me these concentrations work please? Thanks you.

adminSubmitted [ Oct 12 2018 ]

Answered: Hi Vey, The infrmation you need is confidential. May I know why do you need this information? Thank you. Best regards, Elabscience

... Show All Show Less

*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha