Current Position:Home>>Kits>>ELISA Kits>> Rat D-LDH(D-Lactate Dehydrogenase) ELISA Kit

Rat D-LDH(D-Lactate Dehydrogenase) ELISA Kit

Cat.No.:E-EL-R0338
Reactivity: Rat

Filter:

ELISA

To Purchase E-EL-R0338

Size:

96T
24T

Price: $495

Qty:

Welcome to order from local distributors.

Protocols for ELISA

FAQ for ELISA

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T
Assay time	3.5h
Detection range	0.16-10 pmol/mL
Sensitivity	0.10 pmol/mL
Sample type &Sample volume	Serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat D-LDH in samples. No significant cross-reactivity or interference between Rat D-LDH and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat D-LDH concentrations in Serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat D-LDH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat D-LDH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat D-LDH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat D-LDH. You can calculate the concentration of Rat D-LDH in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat D-LDH were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat D-LDH were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pmol/mL)	0.50	1.20	3.60	0.50	1.10	3.60
Standard deviation	0.03	0.07	0.13	0.04	0.06	0.19
CV (%)	6.00	5.83	3.61	8.00	5.45	5.28

Recovery

The recovery of Rat D-LDH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	92-104	99
EDTA plasma (n=8)	93-107	99
Cell culture media (n=8)	86-99	91

Target Information

Synonyms

DLD, LDHD, Probable D-lactate dehydrogenase,mitochondrial,

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Antioxidants (2022) IF: 6.313

Therapeutic Effects of Bee Bread on Obesity-Induced Testicular-Derived Oxidative Stress, Inflammation, and Apoptosis in High-Fat Diet Obese Rat Model

DOI: 10.3390/antiox11020255
PMID: 35204140	Sample: Testis

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021) IF: 6.953

Lycium barbarum polysaccharide combined with aerobic exercise ameliorated nonalcoholic fatty liver disease through restoring gut microbiota, intestinal barrier and inhibiting hepatic inflammation

DOI: 10.1016/j.ijbiomac.2021.05.066
PMID: 33992651	Sample: Plasma

Frontiers in Cardiovascular Medicine (2022) IF: 5.846

Adenosine A2a Receptor Regulates Autophagy Flux and Apoptosis to Alleviate Ischemia-Reperfusion Injury via the cAMP/PKA Signaling Pathway

DOI: 10.3389/fcvm.2022.755619
	Sample: cardiomyocytes

INFLAMMATION (2021) IF: 4.092

MiR-708-3p Alleviates Inflammation and Myocardial Injury After Myocardial Infarction by Suppressing ADAM17 Expression

DOI: 10.1007/s10753-020-01404-9
PMID: 33492555	Sample: Cell culture supernatant

Annals of Translational Medicine (2022) IF: 3.616

Activation of AMPK/p38/Nrf2 is involved in resveratrol alleviating myocardial ischemia-reperfusion injury in diabetic rats as an endogenous antioxidant stress feedback

DOI: 10.21037/atm-22-3789
PMID: 36111006	Sample: serum

Frontiers in Pharmacology (2019) IF: 3.845

Angiotensin II Type I Receptor Antagonism Attenuates Nicotine-Induced Cardiac Remodeling, Dysfunction, and Aggravation of Myocardial Ischemia-Reperfusion Injury in Rats

DOI: 10.3389/fphar.2019.01493
	Sample: heart

INTERNATIONAL JOURNAL OF NEUROSCIENCE (2020) IF: 2.107

Aging protects rat cortical slices against to oxygen-glucose deprivation induced damage

DOI: 10.1080/00207454.2020.1730830
	Sample: cortical

ACS Omega (2020) IF: 2.87

Hydroxysafflor Yellow A Together with Blood–Brain Barrier Regulator Lexiscan for Cerebral Ischemia Reperfusion Injury Treatment

DOI: 10.1021/acsomega.0c02502
	Sample: Tissue homogenate

MACROMOLECULAR BIOSCIENCE (2019) IF: 2.895

Anti‐Adhesive, Platelet Gathering Effects of c‐RGD Modified Poly(p‐dioxanone‐co‐l‐Phe) Electrospun Membrane and Its Comprehensive Application in Intestinal Anastomosis

DOI: 10.1002/mabi.201900344
	Sample: plasma

Molecular Medicine Reports (2015) IF: 1.554

Candesartan ameliorates acute myocardial infarction in rats through inducible nitric oxide synthase, nuclear factor‑κB, monocyte chemoattractant protein‑1, activator protein‑1 and restoration of heat shock protein 72

DOI: 10.3892/mmr.2015.4432
	Sample: Serum

Molecular Medicine Reports (2017) IF: 1.692

Protective effect of diethylcarbamazine inhibits NF-κB activation in isoproterenol-induced acute myocardial infarction rat model through the PARP pathway

DOI: 10.3892/mmr.2017.6695
	Sample: Serum

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha