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Cat.No.:E-EL-R2466
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Assay type | Sandwich-ELISA |
Format | 96T |
Assay time | 3.5h |
Detection range | 0.31-20 ng/mL |
Sensitivity | 0.19 ng/mL |
Sample type &Sample volume | cell culture supernatant and tissue homogenate; 100μL |
Specificity | This kit recognizes Rat INS in samples. No significant cross-reactivity or interference between Rat INS and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Rat INS concentrations in cell culture supernatant and tissue homogenate. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
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Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
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Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat INS were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat INS were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (ng/mL) | 0.99 | 2.68 | 6.73 | 1.00 | 2.82 | 6.90 |
Standard deviation | 0.06 | 0.14 | 0.25 | 0.07 | 0.16 | 0.35 |
CV (%) | 6.06 | 5.22 | 3.71 | 7.00 | 5.67 | 5.07 |
The recovery of Rat INS spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Cell culture media (n=8) | 86-98 | 92 |
Samples were spiked with high concentrations of Rat INS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
PMID: 33992387 |
Sample: Tissue homogenate |
Sample: INS-1 cells |
DOI: 10.3390/v14122781 |
|
Sample: INS-1 cells |
PMID: 33684458 |
Sample: Tissue homogenate |
PMID: 34547407 |
Sample: Pancreatic β cell |
Sample: RIN-m5F cell |
PMID: 34229480 |
Sample: serum,testis |
DOI: 10.3390/ijms21197161 |
|
PMID: 32998359 |
Sample: Cell lysate |
DOI: 10.3390/biom8040149 |
|
PMID: 30445784 |
Sample: Cell culture medium |
Sample: Cell culture supernatant |
DOI: 10.1159/000480395 |
|
Sample: Urine,Tissue homogenate,Plasma |
PMID: 32803305 |
Sample: Cell culture supernatant |
Sample: Serum,plasma |
PMID: 31356906 |
Sample: Serum,Tissue homogenate |
Sample: INS-1 (832/13) cells |
DOI: 10.1042/BSR20180644 |
|
Sample: Cell culture supernatant |
DOI: 10.1055/a-1261-5282 |
|
Sample: INS-1 (832/13) cell |
DOI: 10.1155/2017/4107943 |
|
Sample: Cell culture supernatant |
Sample: INS -1 (832/13) cells |
PMID: 31938037 |
Sample: Serum,Cell culture medium |
DOI: 10.1002/fsn3.988 |
|
Sample: Tissue homogenate,Serum |
PMID: 32963600 |
Sample: Tissue homogenate |
Sample: INS-1 (832/13) cells |
DOI: 10.1055/a-1014-2544 |
|
PMID: 31614370 |
Sample: Cell culture supernatant |
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