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Recombinant SARS-CoV-2 Spike/RBD Protein (RBD, His Tag)

  • Cat.No.:PKSR030507

  • Expression host: Baculovirus-Insect Cells

To Purchase PKSR030507

Size:
  • 100µg
Price: $1105
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Description

Synonyms 2019-nCov S protein;2019-nCoV Spike glycoprotein;2019-nCoV S glycoprotein
Species SARS-CoV-2
Expression_host Baculovirus-Insect Cells
Sequence Arg306-Phe527
Accession AAX16192.1
Application Immunogen(E-AB-V1002,E-AB-V1003,E-AB-V1005)
Mol_Mass 26.5 kDa
AP_Mol_Mass 35.1 kDa
Tag C-His

Properties

Purity > 95 % as determined by reducing SDS-PAGE.
Endotoxin level < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation LyopHilized from sterile 20 mM PBS,300 mM NaCl,10% glycerol,pH 7.0
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.

Background

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

Citations

Reviews/Q&A

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  • Q&A
Q

T****aSubmitted [ Nov 30 2022 ]

Asked: Hello, could you tell which variante of the SARS-CoV-2 this spike protein belongs to? I mean for example like alpha, delta, omikron...? Or is this not possible?

Reply

A

adminSubmitted [ Nov 30 2022 ]

Answered: Dear Tamara, we will reply to you by email later, please pay attention to the mailbox.

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