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Recombinant Human CTLA4 Protein (His Tag)

Uniprot : P16410
  • Cat.No.:PKSH033702

  • Expression host: HEK293 Cells

To Purchase PKSH033702

Size:
  • 10µg
  • 50µg
Price: $62
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Description

Synonyms Cytotoxic T-lymphocyte protein 4;Cytotoxic T-lymphocyte-associated antigen 4;CTLA4;CD152;Cytotoxic T-Lymphocyte-Associated Protein 4
Species Human
Expression_host HEK293 Cells
Sequence Lys36-Asp161
Accession P16410
Application Functional ELISA
Mol_Mass 14.3 kDa
AP_Mol_Mass 20-25 kDa
Tag C-His
Bio_Activity Immobilized Mouse B7-1-Fc(Cat: PKSM041366) at 2μg/ml(100 μl/well) can bind Human CTLA-4-His. The ED50 of Human CTLA-4-His is 5-50 ng/ml.

Properties

Purity > 95 % as determined by reducing SDS-PAGE.
Endotoxin level < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.

Background

Cytotoxic Tlymphocyte 4(CTLA-4;CD152); is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily. Human or mouse CTLA4 cDNA encodes 223 amino acids (aa) including a 35 aa signal sequence; a 126 aa extracellular domain (ECD) with one Ig-like V-type domain; a 21 aa transmembrane (TM) sequence; and a 41 aa cytoplasmic sequence.It is widely expressed with highest levels in lymphoid tissues. CD28 and CTLA-4; together with their ligands; B7-1 and B7-2; constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. CTLA4 transmits an inhibitory signal to T cells; whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T Cells and may play an important role in their functions. Tcell activation through the Tcell receptor and CD28 leads to increased expression of CTLA4.

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