Synonyms	Lymphocyte activation gene 3 protein;LAG3;LAG-3;Protein FDC;CD223;LAG-3
Species	Human
Expression_host	HEK293 Cells
Sequence	Leu23-Leu450
Accession	P18627
Application	Functional ELISA
Mol_Mass	47.2 kDa
AP_Mol_Mass	60-80 kDa
Tag	C-His
Bio_Activity	Immobilized Human LAG-3-His at 5μg/ml (100 μl/well) can bind Human FGL1-mFc.The ED50 of Recombinant Human FGL1-mFc is 30-90 ng/ml.

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin level	< 1.0 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, 2mM EDTA, pH7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background

Human Lymphocyte activation gene 3 protein( LAG3) is a member of immunoglobulin (Ig) superfamily. LAG3 contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. LAG3 is involved in lymphocyte activation and can bind to HLA class-II antigens. It is selectively expressed in activated T and NK cells. LAG3 has a negative regulatory function in T cells and acts as as a new marker of T cell induced B cell activation. As a soluble molecule; LAG3 activates antigen-presenting cells through MHC class II signaling. It can lead to increased antigen-specific T-cell responses in vivo. LAG-3 has higher affinity to MHC class II than CD4.

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