Current Position:Home>>Kits>>ELISA Kits>> ST/5-HT(Serotonin/5-Hydroxytryptamine) ELISA Kit

ST/5-HT(Serotonin/5-Hydroxytryptamine) ELISA Kit

Cat.No.:E-EL-0033
Reactivity: Universal

Filter:

ELISA

To Purchase E-EL-0033

Size:

96T
48T
24T
96T*5
96T*10

Price: $495

Qty:

Welcome to order from local distributors.

Protocols for ELISA

FAQ for ELISA

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T
Assay time	2.0h
Detection range	15.63-1000 ng/mL
Sensitivity	9.38 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 50μL
Specificity	This kit recognizes ST/5-HT in samples. No significant cross-reactivity or interference between ST/5-HT and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of ST/5-HT concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ST/5-HT. During the reaction, ST/5-HT in the sample or standard competes with a fixed amount of ST/5-HT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to ST/5-HT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ST/5-HT in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level ST/5-HT were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level ST/5-HT were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	51.20	141.30	362.70	54.70	132.00	351.00
Standard deviation	3.50	5.90	15.60	3.10	6.50	16.10
CV (%)	6.84	4.18	4.30	5.67	4.92	4.59

Recovery

The recovery of ST/5-HT spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	95-107	100
EDTA plasma(n=8)	86-99	92
Cell culture media(n=8)	91-105	98

Linearity

Samples were spiked with high concentrations of ST/5-HT and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	5HT, Enteramine, Thrombocytin, Thrombotonin, 3-(β-Aminoethyl)-5-hydroxyindole
Research Area	Neuroscience

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Citations

GASTROENTEROLOGY (2017) IF: 18.392

Increased Serotonin Signaling Contributes to the Warburg Effect in Pancreatic Tumor Cells Under Metabolic Stress and Promotes Growth of Pancreatic Tumors in Mice

DOI: 10.1053/j.gastro.2017.03.008
PMID: 28315323	Sample: Cell culture supernatant,Tissue homogenate,Plasma

Cancer Immunology Research (2021) IF: 11.151

Overproduction of Gastrointestinal 5-HT Promotes Colitis-Associated Colorectal Cancer Progression via Enhancing NLRP3 Inflammasome Activation

DOI: 10.1158/2326-6066.CIR-20-1043
	Sample: NCM460 cell,SW480 cell,SW620 cell,HCT116 cell,HT29

Science China-Life Sciences (2022) IF: 10.372

Decreased peripheral mtDNA in methamphetamine use disorder

DOI: 10.1007/s11427-021-2027-1
	Sample: blood

ENVIRONMENTAL POLLUTION (2021) IF: 8.071

Chronic cereulide exposure causes intestinal inflammation and gut microbiota dysbiosis in mice

DOI: 10.1016/j.envpol.2021.117814
	Sample: serum

BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 7.419

Chaihujialonggumulitang shows psycho-cardiology therapeutic effect on acute myocardial infarction with comorbid anxiety by the activation of Nrf2/HO-1 pathway and suppression of oxidative stress and apoptosis

DOI: 10.1016/j.biopha.2022.113437
PMID: 36076489	Sample: hippocampus tissue

BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 7.419

Attenuation of hyperglycemia-associated dyslipidemic, oxidative, cognitive, and inflammatory crises via modulation of neuronal ChEs/NF-κB/COX-2/NOx, and hepatorenal functional deficits by the Tridax procumbens extract

DOI: 10.1016/j.biopha.2022.114114
	Sample: brain

Frontiers in Immunology (2021) IF: 7.561

Lactobacillus plantarum CCFM1143 Alleviates Chronic Diarrhea via Inflammation Regulation and Gut Microbiota Modulation: A Double-Blind, Randomized, Placebo-Controlled Study

DOI: 10.3389/fimmu.2021.746585
PMID: 34721416	Sample: serum

PHARMACOLOGICAL RESEARCH (2022) IF: 7.658

Lactobacillus rhamnosus GG colonization in early life regulates gut-brain axis and relieves anxiety-like behavior in adulthood

DOI: 10.1016/j.phrs.2022.106090
PMID: 35065201	Sample: colon tissue

Frontiers in Cellular and Infection Microbiology (2022) IF: 6.073

Changes in intestinal microflora and its metabolites underlie the cognitive impairment in preterm rats

DOI: 10.3389/fcimb.2022.945851
PMID: 36061856	Sample: serum

Frontiers in Oncology (2021) IF: 6.244

Dopamine Pathway Mediated by DRD5 Facilitates Tumor Growth via Enhancing Warburg Effect in Esophageal Cancer

DOI: 10.3389/fonc.2021.655861
	Sample: Esophageal Cancer tissue

BIOMEDICINE & PHARMACOTHERAPY (2019) IF: 6.53

Metabolomic evidence for the therapeutic effect of gentiopicroside in a corticosterone-induced model of depression

DOI: 10.1016/j.biopha.2019.109549
	Sample: brain

MOLECULAR NUTRITION & FOOD RESEARCH (2022) IF: 6.575

Branched Chain Amino Acids, New Target of Germinated Brown Rice against Type 2 Diabetes Mellitus: A Randomized Controlled Trial

DOI: 10.1002/mnfr.202200481
	Sample: serum

Nutrients (2022) IF: 6.706

Prevention of Loperamide-Induced Constipation in Mice and Alteration of 5-Hydroxytryotamine Signaling by Ligilactobacillus salivarius Li01

DOI: 10.3390/nu14194083
PMID: 36235735	Sample: serum,colonic tissue

Journal of Animal Science and Biotechnology (2022) IF: 5.032

Constant light in early life induces fear-related behavior in chickens with suppressed melatonin secretion and disrupted hippocampal expression of clock- and BDNF-associated genes

DOI: 10.1186/s40104-022-00720-4
PMID: 35729672	Sample: serum

Pharmaceuticals (2022) IF: 5.215

Neuroprotective Effect of Morin Hydrate against Attention-Deficit/Hyperactivity Disorder (ADHD) Induced by MSG and/or Protein Malnutrition in Rat Pups: Effect on Oxidative/Monoamines/Inflammatory Balance and Apoptosis

DOI: 10.3390/ph15081012
PMID: 36015160	Sample: brain

PHYTOMEDICINE (2021) IF: 5.34

Extracts of Poria cocos improve functional dyspepsia via regulating brain-gut peptides, immunity and repairing of gastrointestinal mucosa

DOI: 10.1016/j.phymed.2021.153875
PMID: 34911003	Sample: serum

Food & Function (2021) IF: 5.396

Neuroprotective effects of macamide from maca (Lepidium meyenii Walp.) on corticosterone-induced hippocampal impairments through anti-inflammatory, neurotrophic, and synaptic protection properties

DOI: 10.1039/D1FO01720A
	Sample: hippocampu

Food & Function (2022) IF: 5.396

A randomised, double-blind, placebo-controlled trial of Bifidobacterium bifidum CCFM16 for manipulation of gut microbiota and relief from chronic constipation

DOI: 10.1039/D1FO03896F
	Sample: serum

MEDICINE AND SCIENCE IN SPORTS AND EXERCISE (2022) IF: 5.411

Regular Aerobic Exercise Attenuates Pain and Anxiety in Mice by Restoring Serotonin-Modulated Synaptic Plasticity in the Anterior Cingulate Cortex.

DOI: 10.1249/mss.0000000000002841
	Sample: brain

Foods (2023) IF: 5.561

Fermented Wheat Germ Alleviates Depression-like Behavior in Rats with Chronic and Unpredictable Mild Stress

DOI: 10.3390/foods12050920
	Sample: hippocampus

VASCULAR PHARMACOLOGY (2021) IF: 5.773

Serotonin and chronic hypoxic pulmonary hypertension activate a NADPH oxidase 4 and TRPM2 dependent pathway for pulmonary arterial smooth muscle cell proliferation and migration

DOI: 10.1016/j.vph.2021.106860
PMID: 33794383	Sample: Whole Blood,Cell sample

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 5.924

Receptor-Mediated AKT/PI3K Signalling and Behavioural Alterations in Zebrafish Larvae Reveal Association between Schizophrenia and Opioid Use Disorder

DOI: 10.3390/ijms23094715
PMID: 35563106	Sample: larvae

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (2022) IF: 4.114

Protective effect of low-dose radiation on doxorubicin-induced brain injury in mice

DOI: 10.1016/j.abb.2022.109390
PMID: 36067878	Sample: brain tissue

Frontiers in Psychiatry (2022) IF: 4.157

Integrated Functional Neuroimaging, Monoamine Neurotransmitters, and Behavioral Score on Depressive Tendency in Intensive Care Unit Medical Staffs Induced by Sleep Deprivation After Night Shift Work

DOI: 10.3389/fpsyt.2022.848709
PMID: 35392383	Sample: serum

MITOCHONDRION (2021) IF: 4.16

Yoga improves mitochondrial health and reduces severity of autoimmune inflammatory arthritis: A randomized controlled trial

DOI: 10.1016/j.mito.2021.03.004
PMID: 33741520	Sample: serum

Food & Function (2020) IF: 4.171

Inulin ameliorates schizophrenia via modulating gut microbiota and anti-inflammation in mice

DOI: 10.1039/D0FO02778B
	Sample: The supernatants of the brain tissue

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2019) IF: 4.183

Deep-Fried Atractylodis Rhizoma Protects against Spleen Deficiency-Induced Diarrhea through Regulating Intestinal Inflammatory Response and Gut Microbiota

DOI: 10.3390/ijms21010124
	Sample: serum

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021) IF: 4.223

Impact of ginseng on neurotoxicity induced by cisplatin in rats

DOI: 10.1007/s11356-021-16403-y
	Sample: brain

PHYTOMEDICINE (2020) IF: 4.268

DOI: 10.1016/j.phymed.2020.153269
	Sample: hippocampus

JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36

The mechanisms of action of WeiChang'An Pill (WCAP) treat diarrhoea-predominant irritable bowel syndrome (IBS-D) using network pharmacology approach and in vivo studies

DOI: 10.1016/j.jep.2021.114119
PMID: 33862102	Sample: Serum

JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36

Protective effect of the combination of essential oil from patchouli and tangerine peel against gastric ulcer in rats

DOI: 10.1016/j.jep.2021.114645
PMID: 34530094	Sample: serum

JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36

Biochemical analysis reveals the systematic response of motion sickness mice to ginger (Zingiber officinale) extract's amelioration effect

DOI: 10.1016/j.jep.2022.115077
PMID: 35131339	Sample: blood

JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36

Atractylodes oil alleviates diarrhea-predominant irritable bowel syndrome by regulating intestinal inflammation and intestinal barrier via SCF/c-kit and MLCK/MLC2 pathways

DOI: 10.1016/j.jep.2021.113925
PMID: 33592255	Sample: Serum,Tissue homogenate(colon)

Molecular Brain (2023) IF: 4.399

Investigating the molecular mechanisms of delirium-like neuropsychiatric disorder induced by electromagnetic pulse based on bioinformatics analysis

DOI: 10.1186/s13041-023-00998-z
	Sample: prefrontal cortex,hippocampus

MOLECULES (2020) IF: 4.412

The Effect of Protein-Rich Extract from Bombyx Batryticatus against Glutamate-Damaged PC12 Cells Via Regulating γ-Aminobutyric Acid Signaling Pathway

DOI: 10.3390/molecules25030553
	Sample: PC12 Cell

Antioxidants (2019) IF: 4.52

Neuroprotective Effects of Cornus officinalis on Stress-Induced Hippocampal Deficits in Rats and H2O2-Induced Neurotoxicity in SH-SY5Y Neuroblastoma Cells

DOI: 10.3390/antiox9010027
	Sample: serum

ZOOLOGICAL RESEARCH (2021) IF: 4.56

Fluoxetine ameliorates depressive symptoms by regulating lncRNA expression in the mouse hippocampus

DOI: 10.24272/j.issn.2095-8137.2020.294
PMID: 33420763	Sample: Tissue homogenate

JOURNAL OF PSYCHIATRIC RESEARCH (2021) IF: 4.791

Fecal microbiota transplantation ameliorates stress-induced depression-like behaviors associated with the inhibition of glial and NLRP3 inflammasome in rat brain

DOI: 10.1016/j.jpsychires.2021.02.057
PMID: 33677218	Sample: cortex,hippocampus

Oxidative Medicine and Cellular Longevity (2019) IF: 4.868

Reactive Oxygen Species Are Involved in the Development of Gastric Cancer and Gastric Cancer-Related Depression through ABL1-Mediated Inflammation Signaling Pathway

DOI: 10.1155/2019/5813985
	Sample: blood

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2020) IF: 4.872

Neuromodulatory effect of cinnamon oil on behavioural disturbance, CYP1A1, iNOStranscripts and neurochemical alterations induced by deltamethrin in rat brain

DOI: 10.1016/j.ecoenv.2020.111820
PMID: 33385678	Sample: brain

Journal of Neurogastroenterology and Motility (2021) IF: 4.924

A Pilot Study of Clinical Evaluation and Formation Mechanism of Irritable Bowel Syndrome-like Symptoms in Inflammatory Bowel Disease Patients in Remission

DOI: 10.5056/jnm20151
PMID: 34642282	Sample: serum

INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 4.932

Atractyloside-A ameliorates spleen deficiency diarrhea by interfering with TLR4/MyD88/NF-κB signaling activation and regulating intestinal flora homeostasis

DOI: 10.1016/j.intimp.2022.108679
PMID: 35279514	Sample: serum

Scientific Reports (2022) IF: 4.996

Investigation of acute organophosphate poisoning in humans based on sociodemographic and role of neurotransmitters with survival study in South India

DOI: 10.1038/s41598-022-21054-1
PMID: 36192626	Sample: plasma

Scientific Reports (2023) IF: 4.996

Gallic acid abates cadmium chloride toxicity via alteration of neurotransmitters and modulation of inflammatory markers in Wistar rats

DOI: 10.1038/s41598-023-28893-6
	Sample: brain

JOURNAL OF ETHNOPHARMACOLOGY (2016) IF: 3.055

Venenum Bufonis induces rat neuroinflammation by activiating NF-κB pathway and attenuation of BDNF

DOI: 10.1016/j.jep.2016.03.049
	Sample: Tissue homogenate

JOURNAL OF ETHNOPHARMACOLOGY (2016) IF: 3.055

Anticonvulsant and sedative–hypnotic activity screening of pearl and nacre (mother of pearl)

DOI: 10.1016/j.jep.2016.01.039
	Sample: Tissue homogenate

BRAIN RESEARCH BULLETIN (2019) IF: 3.103

The role of gonadal hormones in the hypoglossal discharge activity of rats exposed to chronic intermittent hypoxia

DOI: 10.1016/j.brainresbull.2019.04.017
	Sample: serum

EUROPEAN JOURNAL OF PHARMACOLOGY (2020) IF: 3.263

Demonstration of an action pathway in mouse platelets leading to prolongation of bleeding time by fluoxetine

DOI: 10.1016/j.ejphar.2020.173385
PMID: 32710955	Sample: Plasma,Platelet

BMC NEUROSCIENCE (2022) IF: 3.264

Cacna2d2 inhibits axonal regeneration following surgical decompression in a rat model of cervical spondylotic myelopathy

DOI: 10.1186/s12868-022-00727-7
PMID: 35778700	Sample: spinal cord tissue

Brain Sciences (2020) IF: 3.332

Antidepressant-Like Properties of Fish Oil on Postpartum Depression-Like Rats Model: Involvement of Serotonergic System

DOI: 10.3390/brainsci10100733
PMID: 33066310	Sample: Oil

EUROPEAN JOURNAL OF NEUROSCIENCE (2021) IF: 3.386

Fecal microbiota transplantation ameliorates gut microbiota imbalance and intestinal barrier damage in rats with stress-induced depressive-like behavior

DOI: 10.1111/ejn.15192
PMID: 33742731	Sample: Serum

International Journal of Environmental Research and Public Health (2021) IF: 3.39

A Case Control Study on Serum Levels of Potential Biomarkers in Male Breast Cancer Patients

DOI: 10.3390/ijerph18094852
PMID: 34062830	Sample: Serum

JOURNAL OF ETHNOPHARMACOLOGY (2019) IF: 3.414

Alkaloids from the rhizomes of Ligusticum striatum exert antimigraine effects through regulating 5-HT1B receptor and c-Jun

DOI: 10.1016/j.jep.2019.03.026
PMID: 30880256	Sample: Tissue homogenate

PHARMACEUTICAL BIOLOGY (2022) IF: 3.503

A comprehensive study on the relieving effect of Lilium brownii on the intestinal flora and metabolic disorder in p-chlorphenylalanine induced insomnia rats

DOI: 10.1080/13880209.2021.2019283
PMID: 34978949	Sample: hypothalamus tissue

Annals of Translational Medicine (2022) IF: 3.616

Mechanism of the Huangguoshu waterfall forest environment's promotive effect on human health in Guizhou, China.

DOI: 10.21037/atm-22-3787
	Sample: serum

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2018) IF: 3.687

The Anti-Stress Effect of Mentha arvensis in Immobilized Rats

DOI: 10.3390/ijms19020355
	Sample: serum

PLoS One (2022) IF: 3.752

Maternal stress induced anxiety-like behavior exacerbated by electromagnetic fields radiation in female rats offspring

DOI: 10.1371/journal.pone.0273206
PMID: 35998127	Sample: hipocampous

Heliyon (2022) IF: 3.776

Anxiolytic and antidepressant-like activities of aqueous extract of Azadirachta indica A. Juss. flower in the stressed rats

DOI: 10.1016/j.heliyon.2022.e08881
	Sample: cortex,hippocampus

NEUROGASTROENTEROLOGY AND MOTILITY (2022) IF: 3.96

The secreted protein P75 of Lactobacillus rhamnosus GG regulates the SERT expression via EGFR and NF-κB pathway

DOI: 10.1111/nmo.14437
	Sample: serum

WATER AIR AND SOIL POLLUTION (2021) IF: 2.52

Effects of Environmental-Related Concentration of Tamoxifen on Hormonal Balance and Reproduction of the Golden Apple Snail, Pomacea canaliculata (Caenogastropoda: Ampullariidae)

DOI: 10.1007/s11270-021-05472-x
	Sample: sexual organ

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2018) IF: 2.559

rLj-RGD3, a novel recombinant toxin protein from Lampetra japonica, prevents coronary thrombosis-induced acute myocardial infarction by inhibiting platelet functions in rats

DOI: 10.1016/j.bbrc.2018.02.021
	Sample: platelets

MICROBIAL PATHOGENESIS (2019) IF: 2.581

Metabolites of stable fly reduce diarrhea in mice by modulating the immune system, antioxidants, and composition of gut microbiota

DOI: 10.1016/j.micpath.2019.103557
	Sample: small intestinal tissues

ENVIRONMENTAL TOXICOLOGY (2019) IF: 2.649

Oxidative stress and neuromodulatory effects of deltamethrin and its combination with insect repellents in rats

DOI: 10.1002/tox.22741
PMID: 30884102	Sample: Serum

MEDICAL SCIENCE MONITOR (2021) IF: 2.649

Human Umbilical Cord Mesenchymal Stem Cell Therapy Mitigates Interstitial Cystitis by Inhibiting Mast Cells

DOI: 10.12659/MSM.930001
PMID: 34354037	Sample: bladder tissue,RBL-2H3 cell

MEDICAL SCIENCE MONITOR (2021) IF: 2.649

Acupuncture Can Play an Antidepressant Role by Regulating the Intestinal Microbes and Neurotransmitters in a Rat Model of Depression

DOI: 10.12659/MSM.929027
PMID: 34039946	Sample: Tissue homogenate,Serum

PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR (2015) IF: 2.781

Antidepressant-like effects of the xanthine oxidase enzyme inhibitor allopurinol in rats. A comparison with fluoxetine

DOI: 10.1016/j.pbb.2015.09.016
	Sample: Plasma

Frontiers in Psychiatry (2020) IF: 2.849

Blue Light Deprivation Produces Depression-Like Responses in Mongolian Gerbils

DOI: 10.3389/fpsyt.2020.00233
	Sample: serum

NEUROGASTROENTEROLOGY AND MOTILITY (2020) IF: 2.946

D‐methionine improves cisplatin‐induced anorexia and dyspepsia syndrome by attenuating intestinal tryptophan hydroxylase 1 activity and increasing plasma leptin concentration

DOI: 10.1111/nmo.13803
	Sample: intestinal tissue

Chinese Medicine (2020) IF: 2.96

Hejie Zhitong prescription promotes sleep and inhibits nociceptive transmission-associated neurotransmitter activity in a rodent migraine model

DOI: 10.1186/s13020-020-00386-y
PMID: 33014123	Sample: Whole Blood

PHARMACEUTICAL BIOLOGY (2020) IF: 2.971

Lilium davidii extract alleviates p‑chlorophenylalanine‑induced insomnia in rats through modification of the hypothalamic-related neurotransmitters, melatonin and homeostasis of the hypothalamic-pituitary-adrenal axis

DOI: 10.1080/13880209.2020.1812674
PMID: 32924742	Sample: brain

FISHERIES SCIENCE (2020) IF: 1.173

Physiological changes in response to social isolation in male medaka fish

DOI: 10.1007/s12562-020-01441-1
	Sample: Tissue homogenate

VETERINARY CLINICAL PATHOLOGY (2022) IF: 1.333

Diagnostic effectiveness of stress biomarkers in cats with feline interstitial and bacterial cystitis

DOI: 10.1111/vcp.13173
	Sample: serum

CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2017) IF: 1.822

Effects of ferulic acid on oxidative stress, heat shock protein 70, connexin 43, and monoamines in the hippocampus of pentylenetetrazole-kindled rats

DOI: 10.1139/cjpp-2016-0219
	Sample: Tissue homogenate

RESTORATIVE NEUROLOGY AND NEUROSCIENCE (2019) IF: 1.839

Impact of yoga based mind-body intervention on systemic inflammatory markers and co-morbid depression in active Rheumatoid arthritis patients: A randomized controlled trial

DOI: 10.3233/RNN-180875
	Sample: serum

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

L************tSubmitted [ Jul 02 2023 ]

Asked: Can I have the question whether this ELISA kit is suitable for Human? because the target kit i need is Serotonin Human Elisa Kit

adminSubmitted [ Jul 02 2023 ]

Answered: Hello, Our E-EL-0033 can work for human samples. The healthy human serum&plasma is about 60-200 ng/mL verified in house, with individual differences and for reference only.

S******************nSubmitted [ May 12 2023 ]

Asked: This kit is suitable for rat thalamus?

adminSubmitted [ May 12 2023 ]

Answered: This kit is suitable for rat thalamus tissue homogenates. Considering the individual differences between samples, it is recommended that a preliminary test can be performed before formal testing.

M***********aSubmitted [ Dec 03 2018 ]

Asked: Please there are two components in Serotonin kit : 1. Concentrated Biotinylated Detection Ab(100x) 120 μL, How can I dilute it with Biotinylated Detection Ab Diluent 14 ml ?, Or how much quality in ( ml ) can I add from Biotinylated Detection Ab Diluent to 120 μL of Concentrated Biotinylated Detection Ab(100x)? 2. Concentrated HRP Conjugate ( 100x ) 120 μL, How can I dilute it with HRP Conjugate Diluent 14 ml? Or how much quality in ( ml ) can I add from HRP Conjugate Diluent to 120 μL Concentrated HRP Conjugate ( 100x )? -- thanx--

adminSubmitted [ Dec 03 2018 ]

Answered: 1.Prepare the 1x Biotinylated Detection Ab working solution by add 9μL of Biotinylated Detection Ab Diluent to each microlitre of Concentrated Biotinylated Detection Ab(100x), the dilution ratio is 9:1. 2. Prepare the 1x HRP Conjugate working solution by add 9μL of HRP Conjugate Diluent to each microlitre of Concentrated HRP Conjugate( 100x ), the dilution ratio is 9:1.

D*****************ySubmitted [ Aug 14 2018 ]

Asked: I Have couple of questions for the 5HT ELISA kit (E-EL-0033) you have: 1. How reliable is the serotonin antibody in terms of cross reactivity with N-acetylserotonin, melatonin, tryptamine and other biological amines? 2. What is the sensitivity as compared to the kits from other popular commercial sources like Enzo, etc? Thanks, Surajit

adminSubmitted [ Aug 14 2018 ]

Answered: 1. Our lab applied 5-HT coupled with BSA as the antigen to generate this kit, the antibody is mouse monoclonal with high specificity, we have also validated this kit with melatonin and tryptamine, almost no cross reactivity for the two analogues was observed. 2. The detection rang is 15.63~1000ng/mL and the sensitivity is 9.38ng/mL for our kit.

e****aSubmitted [ Jul 28 2018 ]

Asked: may i have the question whether this elisa kit is suitable for mouse ?

adminSubmitted [ Jul 28 2018 ]

Answered: Dear eivira, Thanks for your question. This item with cat. #E-EL-0033 is an universal ELISA kit, and it's suitable for mouse. Please feel free to contact us if any question. Thank you.

... Show All Show Less

*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha