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Current Position:Home>>Kits>>Metabolism Assays>> Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit

Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit

Citations(31)

  • Cat.No.:E-BC-K136-S

  • Detection instrument: Spectrophotometer(520 nm)

Manual MSDS

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Detection principle

A variety of antioxidant macromolecules, antioxidant molecules and enzymes in a system can eliminate all kinds of reactive oxygen species and prevent oxidative stress induced by reactive oxygen species. The total level reflect the total antioxidant capacity in the system. Many antioxidants in the body can reduce Fe3+ to Fe2+ and Fe2+ can form stable complexes with phenanthroline substance. The antioxidant capacity (T-AOC) can be calculated by measuring the absorbance at 520 nm.

Performance characteristics

Synonyms T-AOC
Sample type Serum,plasma,saliva,urine,tissue,cells
Sensitivity 0.62 U/mL
Detection range 0.62-145.2 U/mL
Detection method Colorimetric method
Assay type Quantitative
Assay time 80 min
Precision Average inter-assay CV: 8.200%Average intra-assay CV: 2.700%
Other instruments required Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.62-145.2 U/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

10% Mouse liver tissue homogenization

1

10% Mouse lung tissue homogenization

1

Human saliva

1

Human serum

1

Porcine serum

1

Rat plasma

1

HepG2 cells homogenization

1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

  1. Antioxidants (2022) IF: 7.675
    Inhibition of Tumor Growth and Modulation of Antioxidant Activity of Rhodoxanthin Isolated from Taxus baccata Aril against B16F10 Murine Malignant Melanoma

    DOI: 10.3390/antiox11112264
    Sample: plasma
  2. Oxidative Medicine and Cellular Longevity (2020) IF: 5.076
    Ameliorative Effects of Bredemolic Acid on Markers Associated with Renal Dysfunction in a Diet-Induced Prediabetic Rat Model

    DOI: 10.1155/2020/2978340

    PMID: 32655765

    		 Sample: Plasma,Urine,Tissue homogenate
  3. Foods (2023) IF: 5.561
    Treatment of Diabetes Nephropathy in Mice by Germinating Seeds of Euryale ferox through Improving Oxidative Stress

    DOI: 10.3390/foods12040767
    Sample: Serum
  4. Frontiers in Pharmacology (2021) IF: 5.811
    The Anti-Inflammatory Effect of a Combination of Five Compounds From Five Chinese Herbal Medicines Used in the Treatment of COPD

    DOI: 10.3389/fphar.2021.709702
    Sample: serum
  5. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
    Comparison of Selenium Nanoparticles and Sodium Selenite on the Alleviation of Early Atherosclerosis by Inhibiting Endothelial Dysfunction and Inflammation in Apolipoprotein E-Deficient Mice

    DOI: 10.3390/ijms222111612

    PMID: 34769040

    		 Sample: serum
  6. Drug Design Development and Therapy (2021) IF: 4.162
    Role of Oxidative Stress and Reduced Endogenous Hydrogen Sulfide in Diabetic Nephropathy

    DOI: 10.2147/DDDT.S291591

    PMID: 33707940

    		 Sample: Tissue homogenate
  7. Drug Design Development and Therapy (2022) IF: 4.319
    The Preventive and Therapeutic Effects of Ajwa Date Fruit Extract Against Acute Diclofenac Toxicity-Induced Colopathy: An Experimental Study

    DOI: 10.2147/DDDT.S344247

    PMID: 35965961

    		 Sample: serum
  8. JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
    Suhuang antitussive capsule ameliorates post-infectious cough in mice through AhR-Nrf2 pathway

    DOI: 10.1016/j.jep.2021.114664

    PMID: 34555451

    		 Sample: lung tissue
  9. Scientific Reports (2021) IF: 4.38
    Effect of tiger milk mushroom (Lignosus rhinocerus) supplementation on respiratory health, immunity and antioxidant status: an open-label prospective study

    DOI: 10.1038/s41598-021-91256-6

    PMID: 34083710

    		 Sample: saliva
  10. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
    Vitamin E and selenium administration synergistically mitigates ivermectin and doramectin-induced testicular dysfunction in male Wistar albino rats

    DOI: 10.1016/j.biopha.2020.109841

    PMID: 31972360

    		 Sample: Tissue homogenate
  11. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
    Marein ameliorates diabetic nephropathy by inhibiting renal sodium glucose transporter 2 and activating the AMPK signaling pathway in db/db mice and high glucose–treated HK-2 cells

    DOI: 10.1016/j.biopha.2020.110684
    Sample: serum
  12. Food Quality and Safety (2022) IF: 3.102
    Novel Functional Food from an invasive species Polygonum cuspidatum: Safety evaluation, Chemical Composition, and Hepatoprotective Effects

    DOI: 10.1093/fqsafe/fyac032
  13. Drug Design Development and Therapy (2019) IF: 3.208
    Effects of quercetin, sitagliptin alone or in combination in testicular toxicity induced by doxorubicin in rats

    DOI: 10.2147/DDDT.S222127

    PMID: 31571833

    		 Sample: Serum
  14. Animals (2022) IF: 3.231
    Milk Pathogens in Correlation with Inflammatory, Oxidative and Nitrosative Stress Markers in Goat Subclinical Mastitis

    DOI: 10.3390/ani12233245
    Sample: skimmed milk
  15. Biomed Research International (2022) IF: 3.246
    Glycine Improved Cryopreserved Spermatozoa Quality in Achai Bull

    DOI: 10.1155/2022/8282387

    PMID: 35968237

    		 Sample: semen
  16. Frontiers in Veterinary Science (2022) IF: 3.412
    Ovarian Oxidative Stress Induced Follicle Depletion After Zona Pellucida 3 Vaccination Is Associated With Subfertility in BALB/c Mice

    DOI: 10.3389/fvets.2022.814827

    PMID: 35252419

    		 Sample: Ovarian
  17. PHARMACEUTICAL BIOLOGY (2022) IF: 3.503
    Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathway

    DOI: 10.1080/13880209.2022.2070221

    PMID: 35635784

    		 Sample: brain tissue
  18. Journal of Inorganic and Organometallic Polymers and Materials (2023) IF: 3.518
    Preparation of Novel Composites of Polyvinyl Alcohol Containing Hesperidin Loaded ZnO Nanoparticles and Determination of Their Biological and Thermal Properties

    DOI: 10.1007/s10904-023-02532-z
  19. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2022) IF: 3.639
    Flaxseed oil ameliorates aging in D-galactose induced rats via altering gut microbiota and mitigating oxidative damage

    DOI: 10.1002/jsfa.12010

    PMID: 35567370

    		 Sample: serum,liver
  20. JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION (2022) IF: 3.682
    Synergistic effect of ZnO nanoparticles and hesperidin on the antibacterial properties of chitosan

    DOI: 10.1080/09205063.2022.2099668

    PMID: 35797143

    		 Sample: nanocomposites
  21. Scientific Reports (2020) IF: 3.998
    Reproductive effects of subchronic exposure to acetamiprid in male rats

    DOI: 10.1038/s41598-020-65887-0

    PMID: 32488017

    		 Sample: Plasma,Tissue homogenate
  22. International Journal of General Medicine (2023) IF: 2.145
    Hematological, Inflammatory, Coagulation, and Oxidative/Antioxidant Biomarkers as Predictors for Severity and Mortality in COVID-19: A Prospective Cohort-Study

    DOI: 10.2147/IJGM.S402206
    Sample: Serum
  23. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2020) IF: 2.614
    Yacón (Smallanthus sonchifolius) prevented inflammation, oxidative stress, and intestinal alterations in an animal model of colorectal carcinogenesis

    DOI: 10.1002/jsfa.10595

    PMID: 32567144

    		 Sample: Plasma
  24. Reproductive Sciences (2020) IF: 2.616
    Ascorbic Acid and Alpha-Tocopherol Contribute to the Therapy of Polycystic Ovarian Syndrome in Mouse Models

    DOI: 10.1007/s43032-020-00273-9

    PMID: 32725591

    		 Sample: Serum
  25. BIOLOGICAL TRACE ELEMENT RESEARCH (2020) IF: 2.639
    Impact of Coenzyme Q10 and Selenium on Seminal Fluid Parameters and Antioxidant Status in Men with Idiopathic Infertility

    DOI: 10.1007/s12011-020-02251-3
    Sample: Semen
  26. JOURNAL OF FOOD BIOCHEMISTRY (2022) IF: 2.72
    Effect of coconut water and milk on heat stress-induced gastrointestinal tract dysmotility in rats: Role of oxidative stress and inflammatory response

    DOI: 10.1111/jfbc.14129

    PMID: 35298033

    		 Sample: gastric,jejunal, colonic
  27. JOURNAL OF OCCUPATIONAL AND ENVIRONMENTAL MEDICINE (2020) IF: 1.642
    Spirometric Outcomes and Oxidative Stress Among Cement Factory Workers: Results From a Cross-Sectional Study

    DOI: 10.1097/JOM.0000000000001991

    PMID: 32796257

    		 Sample: serum
  28. ANDROLOGIA (2020) IF: 1.951
    Androgenic effect of aqueous leaf extract of Moringa oleifera on Leydig TM3 cells in vitro

    DOI: 10.1111/and.13825

    PMID: 32926461

    		 Sample: Cell lysate
  29. International Journal of Food Science (2023)
    Fabrication of Nutraceutical Beverage from Saffron (Crocus sativus L.) Extract and Studying Its Health Effects

    DOI: 10.1155/2023/7130266
    Sample: serum
  30. Toxicology Reports (2019)
    Elevated reproductive toxicity effects of diclofenac after withdrawal: Investigation of the therapeutic role of melatonin

    DOI: 10.1016/j.toxrep.2019.06.009
    Sample: plasma
  31. Journal of Nutrition and Metabolism (2020)
    Digestive Fermentation, Antioxidant Status, and Haemato-Biochemical Indices of Growing Rabbits Fed on Diets Supplemented with Ziziphus spina-christi Leaf

    DOI: 10.1155/2020/9046862

    PMID: 32318290

    		 Sample: Serum
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Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
Q

K**aSubmitted [ Mar 07 2024 ]

Asked: Can I convert the volumes of reagents and sample to run microplate assay instead of using cuvette & spectrophotometer

Reply

A

adminSubmitted [ Mar 07 2024 ]

Answered: Each kit has a specified instrument that applies. It's recommended to follow the operation steps of the manual, and do not convert the instrument at will.

Q

N************sSubmitted [ May 08 2023 ]

Asked: this elisa kit suitable for detection of total antioxidant capacity in penile tissue ? tissue of rat

Reply

A

adminSubmitted [ May 08 2023 ]

Answered: Thanks for your inquiry!I'll reply to you by email later. Pls pay attention to the mailbox.

Q

N****mSubmitted [ Mar 21 2023 ]

Asked: Hi to see the enzymatic antioxidant NOS increased in blood but I assayed neuronal NOS 1 . Does it carry any relevance with melasma. 2 want to understand how to calculate enzyme activity through Elisa kit . Like H1113,,H0742 and SOD1 . Please help me to understand What should be V1,V2 and what is control is it standard blank What is time of change in Absorbance let’s please help .

Reply

A

adminSubmitted [ Mar 21 2023 ]

Answered: These kits measure the concentration of the target, not activity. In general, there is no conversion between activity and concentration. Unfortunately, we do not know how these three targets convert concentration into activity at present. E-EL-5410, E-EL-H0742 and E-EL-H 1113 are all kits for double antibody sandwich principle detection, that is, concentration and absorbance are in a positive relationship, in which the blank hole is the background hole, and the blank hole needs to be deducted when calculating the concentration, and then the corrected value.

Q

N****mSubmitted [ Jan 23 2023 ]

Asked: How to calculate total antioxidant capacity with Elisa kit.I want t know calculations .

Reply

A

adminSubmitted [ Jan 23 2023 ]

Answered: Calculation formula is included in the manual

Q

B************iSubmitted [ Nov 07 2022 ]

Asked: Hello, please how do I get the value for Cpr when using the formula for tissue sample in my calculations?

Reply

A

adminSubmitted [ Nov 07 2022 ]

Answered: Hi Blessing, Thanks for your enquiry. The Cpr stands for the protein concentration of the detected samples, you need another assay kit to test protein concentrations. Please let us know if we can be of any further assistance.

Q

M*****aSubmitted [ Aug 17 2022 ]

Asked: I can\'t use cuvette tubes for many reasons related to availability of it and spectrometer. Can I use 96 plate instead please?

Reply

A

adminSubmitted [ Aug 17 2022 ]

Answered: I'll never recommend you to do so. But there is E-BC-K136-M which is designed for 96-well plate specifically.

Q

S**** Submitted [ Mar 21 2021 ]

Asked: Is there is a video how to use the kit?

Reply

A

adminSubmitted [ Mar 21 2021 ]

Answered: Hi Sadiq, The kit manual had been sent to your mailbox for reference. If any question about the experimental procedures, pls feel free to contact me. Britney britney@elabscience.com

Q

S****tSubmitted [ Mar 08 2019 ]

Asked: This kit can use invertebrate hemolymph?

Reply

A

adminSubmitted [ Mar 08 2019 ]

Answered: Dear Serhat, Yes, the kit can use invertebrate hemolymph, if there is other question, please feel free to ask me. Regards, Cathy

Q

fSubmitted [ Dec 01 2018 ]

Asked:  hi is this elisa kit suitable for detection of total antioxidant capacity in small intestine tissue of rat thank you

Reply

A

adminSubmitted [ Dec 01 2018 ]

Answered: This kit can dectect small intestine tissue sample.

Q

F***hSubmitted [ Nov 30 2018 ]

Asked: Hi I want to ask if I want to measure Total antioxidant capacity in small intestine tissue of rat and number of test I require 75 sample can I use this kit and how many kit I require if I use kit for 100 assay thank you

Reply

A

adminSubmitted [ Nov 30 2018 ]

Answered: This kit of 100 assays could detect 50 samples with no duplication. So you need 2 kits of 100 assays if you won't test the samples in duplication.

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