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Total Phenols Colorimetric Assay Kit (Plant Samples)

Cat.No.:E-BC-K354-S
Detection instrument: Spectrophotometer(760 nm)

Detection method: Colorimetric method

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Detection principle

Under alkaline conditions, tungsten-molybdenum acid can be reduced by phenols and produce blue compounds, which has a characteristic absorption peak at 760 nm. The content of total phenols in sample can be calculated indirectly by measuring the absorbance at 760 nm.

Performance characteristics

Sample type	Plant tissue
Sensitivity	0.73 μg/mL
Detection range	0.73-150 μg/mL
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	60 min
Precision	Average inter-assay CV: 2.500%Average intra-assay CV: 1.900%
Other instruments required	Micropipettor, Vacuum dryer, Vortex mixer, Ultrasonic cell grinder, Crusher
Other reagents required	60% Ethanol
Storage	2-8℃
Valid period	12 months

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Nutrients (2022) IF: 6.706

Grifola frondosa Extract Containing Bioactive Components Blocks Skin Fibroblastic Inflammation and Cytotoxicity Caused by Endocrine Disrupting Chemical, Bisphenol A

DOI: 10.3390/nu14183812
PMID: 36145189	Sample: fruit

Reviews/Q&A

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Reviews
Q&A

N***iSubmitted [ Sep 12 2023 ]

Asked: Could we freeze the product in -20 degrees for example to prolong its shelf life?

adminSubmitted [ Sep 12 2023 ]

Answered: It is not recommended to store this product at -20 degrees, for which will affect product performance. Please store at 2-8℃ according to the product instructions.

M**********dSubmitted [ Sep 01 2022 ]

Asked: Is there any requirement for \"Room Temperature\"?

adminSubmitted [ Sep 01 2022 ]

Answered: The normal temperature in our laboratory in 15-30 degree. If there is an extremly low or high temperature in your lab, please contact with our technical support, we can make a pre-test for you.

D********dSubmitted [ Sep 01 2022 ]

Asked: Will EtOH interfere the assay?

adminSubmitted [ Sep 01 2022 ]

Answered: No, it won't. EtOH is also included in the sample preparation part.

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