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Urea (BUN) Colorimetric Assay Kit (Diacetyl Oxime Method)

Cat.No.:E-BC-K329-S
Detection instrument: Spectrophotometer(520 nm)

Detection method: Colorimetric method

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Detection principle

In strong acidic and heating condition, urea can react with diacetyl to form red diazine compound. The depth of color is proportional to the content of urea. Because the instability of the diacetyl, the diacetyl oxime usually react with the strong acid firstly in the reaction system to generate diacetyl, then react with urea to generate the red diazine compound. The reaction equation is as follows:

Performance characteristics

Synonyms	BUN
Sample type	Serum,plasma,saliva,urine
Sensitivity	0.12 mmol/L
Detection range	0.12-15 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	60 min
Precision	Average inter-assay CV: 9.900%Average intra-assay CV: 4.900%
Other instruments required	Vortex mixer, Micropipettor, Incubator， Water bath
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.12-15 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human urine	45-60
Human plasma	1
Human serum	1
Human saliva	1
Mouse serum	1
Rat urine	45-60
Rabbit plasma	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Acta Biomaterialia (2021) IF: 8.9469

An efficient and safe MUC1-dendritic cell-derived exosome conjugate vaccine elicits potent cellular and humoral immunity and tumor inhibition in vivo

DOI: 10.1016/j.actbio.2021.10.041
PMID: 34757230	Sample: serum

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208

Ameliorative Effects of a Rhenium (V) Compound with Uracil-Derived Ligand Markers Associated with Hyperglycaemia-Induced Renal Dysfunction in Diet-Induced Prediabetic Rats

DOI: 10.3390/ijms232315400
	Sample: plasma,urine

Pharmaceutics (2023) IF: 6.525

Ultrasonically Fabricated Beta-Carotene Nanoemulsion: Optimization, Characterization and Evaluation of Combinatorial Effect with Quercetin on Streptozotocin-Induced Diabetic Rat Model

DOI: 10.3390/pharmaceutics15020574
	Sample: serum

Oxidative Medicine and Cellular Longevity (2022) IF: 6.543

Capsaicin Prevents Contrast-Associated Acute Kidney Injury through Activation of Nrf2 in Mice

DOI: 10.1155/2022/1763922
PMID: 35615576	Sample: serum

Frontiers in Pharmacology (2022) IF: 5.811

Stewed Rhubarb Decoction Ameliorates Adenine-Induced Chronic Renal Failure in Mice by Regulating Gut Microbiota Dysbiosis.

DOI: 10.3389/fphar.2022.842720
	Sample: plasma

Drug Design Development and Therapy (2021) IF: 4.162

Maackiain Protects the Kidneys of Type 2 Diabetic Rats via Modulating the Nrf2/HO-1 and TLR4/NF-κB/Caspase-3 Pathways

DOI: 10.2147/DDDT.S326975
PMID: 34703210	Sample: serum

INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH (2022) IF: 4.477

Potential Protective Effects of Ruta Chalepensis L. Extracts Against Gentamicin-Induced Nephrotoxicity via Reduction on Apoptotic, DNA Damage and Oxidative Stress Markers in Mice

DOI: 10.1080/09603123.2022.2105824
PMID: 35917490	Sample: serum

BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545

Marein ameliorates diabetic nephropathy by inhibiting renal sodium glucose transporter 2 and activating the AMPK signaling pathway in db/db mice and high glucose–treated HK-2 cells

DOI: 10.1016/j.biopha.2020.110684
	Sample: serum

Frontiers in Physiology (2023) IF: 4.755

Relative comparison of chronic kidney disease-mineral and bone disorder rat models

DOI: 10.3389/fphys.2023.1083725
	Sample: Serum

International Journal of Environmental Research and Public Health (2022) IF: 3.39

Benefits on Hematological and Biochemical Parameters of a High-Intensity Interval Training Program for a Half-Marathon in Recreational Middle-Aged Women Runners

DOI: 10.3390/ijerph19010498
PMID: 35010758	Sample: plasma

JOURNAL OF PHARMACY AND PHARMACOLOGY (2021) IF: 3.765

Cissus quadrangularis extract attenuates diabetic nephropathy by altering SIRT1/DNMT1 axis

DOI: 10.1093/jpp/rgab078
PMID: 34128987	Sample: serum

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