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Vitamin C (VC) Colorimetric Assay Kit

  • Cat.No.:E-BC-K034-S

  • Detection instrument: Spectrophotometer(533 nm)

To Purchase E-BC-K034-S

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Detection principle

The most obvious chemical activity of VC is that reduce Fe3+ to Fe2+, then promote iron absorption in the intestine, promote the storage and utilization of iron. Fe3+ react immediately with reducing ascorbic acid to form Fe2+. then Fe2+ react with phenanthroline and the color developing reaction occurs. The content of vitamin C in sample can be determined. Measure the OD value and calculate the VC content indirectly.

Performance characteristics

Synonyms VC,Ascorbic acid
Sample type Serum,plasma,tissue
Sensitivity 0.35 μg/mL
Detection range 0.35-20 μg/mL
Detection method Colorimetric method
Assay type Quantitative
Assay time 80 min
Precision Average inter-assay CV: 3.300%Average intra-assay CV: 2.300%
Other instruments required Micropipettor, Vortex mixer, Incubator, Centrifuge
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Absolute ethanol
Storage 2-8℃
Valid period 12 months

Images

The ascorbate concentration in tomato is significantly reduced after being treated with cold pla sma for 1, 5 and 10 minutes respectively. (P<0.0001)

B Adhikari et al investigate the effect of cold plasma in priming modulates growth, redox homeostasis and stress in tomato. Vitamin C level of tomato was determined using VC colorimetric assay kit (E-BC-K034-S).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.35-20 μg/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Human plasma

1

10% Mouse kidney tissue homogenization

1

Rat serum

1

Mouse serum

1

10% Rat liver tissue homogenization

1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

  1. FREE RADICAL BIOLOGY AND MEDICINE (2020) IF: 6.17
    Cold plasma seed priming modulates growth, redox homeostasis and stress response by inducing reactive species in tomato (Solanum lycopersicum)

    DOI: 10.1016/j.freeradbiomed.2020.06.003

    PMID: 32561321

    Sample: Plant Tissue
  2. Nutrients (2023) IF: 6.706
    Re-Visiting Antioxidant Therapy in Murine Advanced Atherosclerosis with Brussels Chicory, a Typical Vegetable in Mediterranean Diets

    DOI: 10.3390/nu15040832

    Sample: serum
  3. Frontiers in Psychiatry (2022) IF: 5.435
    Efficacy of a novel proprietary dietary supplement (TRI 360TM) on psychological symptoms and stress-related quality of life in adult subjects: A randomized controlled clinical trial

    DOI: 10.3389/fpsyt.2022.919284

    PMID: 36032243

    Sample: serum
  4. Scientific Reports (2019) IF: 4.011
    Cold Atmospheric Plasma-Activated Water Irrigation Induces Defense Hormone and Gene expression in Tomato seedlings

    DOI: 10.1038/s41598-019-52646-z

    Sample: leaves,roots
  5. LIFE SCIENCES (2020) IF: 3.647
    Quercetin attenuated oxidative DNA damage through NRF2 signaling pathway in rats with DMH induced colon carcinogenesis

    DOI: 10.1016/j.lfs.2020.117584

    PMID: 32220623

    Sample: Tissue homogenate
  6. International Journal of General Medicine (2023) IF: 2.145
    Hematological, Inflammatory, Coagulation, and Oxidative/Antioxidant Biomarkers as Predictors for Severity and Mortality in COVID-19: A Prospective Cohort-Study

    DOI: 10.2147/IJGM.S402206

    Sample: Serum
  7. Journal of Experimental Zoology Part A-Ecological and Integrative Physiology (2022) IF: 2.693
    Molecular mechanisms underlying ameliorative impact of melatonin against age-dependent chronic arsenic toxicity in rats' brains

    DOI: 10.1002/jez.2603

    Sample: brain
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