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Ascorbate Peroxidase (APX) Activity Assay Kit

Cat.No.:E-BC-K353-S
Detection instrument: Spectrophotometer(290 nm)

Detection method: Colorimetric method

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Detection principle

Ascorbate Peroxidase (APX) can catalyze the reaction between ascorbic acid (ASA) and hydrogen peroxide (H2O2), and ASA can be oxidized to monodehydroascorbic acid (MDASA). The absorbance of solution at 290 nm will decline as the oxidation of ASA. The APX activity can be calculated by detecting the decrease of A290.

Performance characteristics

Synonyms	APX
Sample type	Plant tissue
Sensitivity	0.071 U/g tissue
Detection range	0.071-47 U/g tissue
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	60 min
Precision	Average inter-assay CV: 6.400%Average intra-assay CV: 4.800%
Other instruments required	Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Images

In high light stress situation, the APX activity was significantly reduced in snat1 mutant group. (P<0.05)

H Y Lee et al investigate the effects of melatonin in high light stress tolerance in Arabidopsis thaliana. Ascorbate peroxidase (APX) activity of Arabidopsis leaf was determined using APX activity assay kit (E-BC-K353-S).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.071-47 U/g tissue).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
10% Epipremnum aureum tissue homogenization	1
10% Carrot tissue homogenization	1
10% Green pepper tissue homogenization	1
10% Mushrooms tissue homogenization	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Molecular Plant (2021) IF: 13.164

Redox sensor QSOX1 regulates plant immunity by targeting GSNOR to modulate ROS generation

DOI: 10.1016/j.molp.2021.05.004
PMID: 33962063	Sample: Plant Tissue

JOURNAL OF PINEAL RESEARCH (2018) IF: 11.613

Melatonin induction and its role in high light stress tolerance in Arabidopsis thaliana

DOI: 10.1111/jpi.12504
PMID: 29770489	Sample: Leaf tissue

Frontiers in Plant Science (2022) IF: 6.627

Halotolerant Bacillus altitudinis WR10 improves salt tolerance in wheat via a multi-level mechanism

DOI: 10.3389/fpls.2022.941388
PMID: 35909740	Sample: roots or shoots

PLANT PHYSIOLOGY AND BIOCHEMISTRY (2020) IF: 3.72

Overexpression of ethylene response factor ERF96 gene enhances selenium tolerance in Arabidopsis

DOI: 10.1016/j.plaphy.2020.02.024
PMID: 32097848	Sample: Plant Tissue

Plants-Basel (2021) IF: 3.935

Salinity Effects on Morpho-Physiological and Yield Traits of Soybean (Glycine max L.) as Mediated by Foliar Spray with Brassinolide

DOI: 10.3390/plants10030541
	Sample: leaf

PeerJ (2021) IF: 2.984

Physiological changes and transcript identification in Coreopsis tinctoria Nutt. in early stages of salt stress

DOI: 10.7717/peerj.11888
PMID: 34434650	Sample: seedling

HORTSCIENCE (2021) IF: 1.455

Effects of Salt Stress on Photosynthetic Fluorescence Characteristics, Antioxidant System, and Osmoregulation of Coreopsis tinctoria Nutt.

DOI: 10.21273/HORTSCI15956-21
	Sample: seed

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Reviews/Q&A

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Reviews
Q&A

M*****aSubmitted [ Oct 10 2022 ]

Asked: I bought K353-S (APX kit) and K259-M (PPO kit), could the homogenate medium be universal?

adminSubmitted [ Oct 10 2022 ]

Answered: No, both kits need to use the homogenate of reagent 1 in the corresponding kit, not mixed, and not replaced with anything else.

M*****aSubmitted [ Sep 27 2022 ]

Asked: I bought K353-S (APX kit) and K259-M (PPO kit), could the homogenate medium be universal?

adminSubmitted [ Sep 27 2022 ]

Answered: No, both kits need to use the homogenate of reagent 1 in the corresponding kit, not mixed, and not replaced with anything else.

M*****aSubmitted [ Sep 08 2022 ]

Asked: Same thing to reagent 3 of E-BC-K353-S, it ask to dissolve whole pack of powder, how do i keep part of it?

adminSubmitted [ Sep 08 2022 ]

Answered: For reagent3 of E-BC-K353-S, it's powder, so once it's dissolved, it needs to be used up within 3 days.

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