Caspase 3/7 Substrates(Green)(1mM)

Molt-4 cells were culture with (left) or without (right) 5 μM camptothecin for 4 h and then stained with Caspase 3/7 Substrates (Green) and analyzed by flow cytometry.

The Caspase 3/7 Substrates (Green) are a novel fluorogenic dye that couple the caspase 3/7 recognition sequence (DEVD) to a high affinity DNA dye, which have cell membrane permeability and can penetrate the plasma membrane into the cytoplasm. The substrate itself has no fluorescence and has a charge exclusion effect with DNA. During cell apoptosis, caspase-3/7 cleaves the substrate and releases high affinity DNA dyes, which bind to DNA to produce strong fluorescence, thereby detecting caspase-3/7 activity and visualizing the morphological changes of the cell nucleus during apoptosis.