Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> Elab Fluor® Violet 450 Anti-Mouse CD14 Antibody[Sa14-2]
Cat.No.:E-AB-F1176Q
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Alternate Names | CD 14,Myeloid cell-specific leucine-rich glycoprotein,Monocyte differentiation antigen CD14 |
Swissprot | P10810 |
References |
1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Akashi S, et al. 2003. J. Exp. Med. 198:1035. 3. Matsuura K, et al. 1994. J. Exp. Med. 179:1671. 4. Liu S, et al. 1998. Infect. Immun. 66:5089. |
Form | Liquid |
Clone No. | Sa14-2 |
Host | Rat |
Isotype | Rat IgG2a, κ |
Isotype Control | Elab Fluor® Violet 450 Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09832Q] |
Reactivity | Mouse |
Application | FCM |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | Elab Fluor® Violet 450 |
Elab Fluor® Violet 450 is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450 nm (e.g., a 450/45 nm bandpass filter). | |
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How to select the appropriate detection channel through the spectrogram?
CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed on macrophages, dendritic cells, Kupffer cells, hepatocytes, and granulocytes. As a high-affinity receptor for LPS-LBP (LPS-binding protein) complex, CD14, in association with Toll-like Receptor 4 (TLR4) or 2 (TLR2), is involved in the clearance of gram-negative pathogens. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |