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Free Fatty Acids (NEFA/FFA) Fluorometric Assay Kit

  • Cat.No.:E-BC-F039

  • Detection instrument: Fluorescence microplate reader (Ex/Em=535 nm/590 nm)

To Purchase E-BC-F039

Size:
  • 96T
  • 48T
Price: $450
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Protocols for metabolism assays

FAQ for metabolism assays

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Detection principle

Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen peroxide in the presence of acyl oxidase. In the presence of the enzyme and probe, hydrogen peroxide react to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is directly proportional to the concentration of free fatty acids.

Performance characteristics

Synonyms FFA/NEFA
Sample type Serum, plasma, animal tissue, cells
Sensitivity 0.58 μmol/L
Detection range 0.58-20 μmol/L
Detection method Fluorescence method
Assay type Quantitative
Assay time 70 min
Precision Average inter-assay CV: 5.300%Average intra-assay CV: 4.200%
Other instruments required Micropipette, Vortex mixer, Centrifuge
Storage -20℃
Valid period 12 months

Images

The concentration of FFA in rat plasma was significantly increased in T2DM and PPG treated group, and decreased with ALA treatment.

G J Dugbartey et al investigate liver injury induced by diabetes. Free Fatty Acid (FFA) concentration in rat plasma was determined using FFA fluorometric assay kit (E-BC-F039).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.58-20 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

10-20

Rat serum

200-400

Mouse plasma

100-300

Rabbit serum

100-300

10% Rat liver tissue homogenate

200-400

10% Mouse kidney tissue homogenate

200-400

10% Rat brain tissue homogenate

200-400

10% Rat lung tissue homogenate

200-400

Note: The diluent is reagent 1.

Citations

  1. Nature Communications (2022) IF: 17.694
    Pharmacological inhibition of Lin28 promotes ketogenesis and restores lipid homeostasis in models of non-alcoholic fatty liver disease

    DOI: 10.1038/s41467-022-35481-1

    PMID: 36572670

    Sample: serum
  2. CELL PROLIFERATION (2022) IF: 8.7550
    Hypothalamic Hnscr regulates glucose balance by mediating central inflammation and insulin signal

    DOI: 10.1111/cpr.13332

    Sample: liver
  3. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 5.195
    Rhamnogalacturonan-I enriched pectin from steamed ginseng ameliorates lipid metabolism in type 2 diabetic rats via gut microbiota and AMPK pathway

    DOI: 10.1016/j.jep.2022.115862

    PMID: 36283638

    Sample: serum
  4. Diabetology & Metabolic Syndrome (2022) IF: 5.395
    Targeting hepatic sulfane sulfur/hydrogen sulfide signaling pathway with α-lipoic acid to prevent diabetes-induced liver injury via upregulating hepatic CSE/3-MST expression

    DOI: 10.1186/s13098-022-00921-x

    PMID: 36229864

    Sample: Plasma
  5. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021) IF: 4.223
    Biochemical investigation of human exposure to aflatoxin M1 and its association with risk factors of diabetes mellitus

    DOI: 10.1007/s11356-021-14871-w

    PMID: 34216342

    Sample: serum
  6. JOURNAL OF NUTRITIONAL BIOCHEMISTRY (2019) IF: 4.49
    Association between duration of obesity and severity of ovarian dysfunction in rat-cafeteria diet approach

    DOI: 10.1016/j.jnutbio.2019.05.012

    Sample: serum
  7. DRUG AND CHEMICAL TOXICOLOGY (2020) IF: 2.405
    Dexamethasone increases renal free fatty acids and xanthine oxidase activity in female rats: could there be any gestational impact?

    DOI: 10.1080/01480545.2020.1784190

    PMID: 32597234

    Sample: Plasma
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Reviews/Q&A

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  • Reviews
  • Q&A
Q

S*****nSubmitted [ Apr 01 2023 ]

Asked: Does this assay work for the quantification of hexanoic acid?

Reply

A

adminSubmitted [ Apr 01 2023 ]

Answered: This kit is used to detect all free fatty acids in sample, it can not detect hexanoic acid content.

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