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Glucose Uptake Fluorometric Assay Kit

  • Cat.No.:E-BC-F041

  • Detection instrument: Fluorescence microplate reader (Ex/Em=530 nm/590 nm)

To Purchase E-BC-F041

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  • 96T
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Detection principle

2-DG is up-taken by the cells, converted to 2-DG-6P, which is catalyzed by glucose dehydrogenase to produce 6PDG. Meanwhile, NADP+ is converted to NADPH. The generated NADPH converts the probe into fluorescent substances under the action of myocardial yellow transferase. The glucose uptake can be calculated by measuring the fluorescence intensity at the excitation wavelength of 530 nm and the emission wavelength of 590 nm.

Performance characteristics

Sample type cells
Sensitivity 0.02 nmol/μL
Detection range 0.02-0.3 nmol/μL
Detection method Fluorescence method
Assay type Quantitative
Assay time 100 min
Other instruments required Micropipettor, Incubator, Water bath
Other reagents required KRPH solution (20 mM Hepes, 5 mM KH2PO4, 1 mM MgSO4, 1 mM CaCl2, 136 mM NaCl, 4.7 mM KCl, 2% BSA, pH 7.4)
Storage -20℃
Valid period 12 months

Images

Rates of glucose consumption were significantly decreased by shRNA transfection in both kinds of cells. (*P<0.05)

T Cheng et al investigate the function of circGOLPH3 in oral squamous cell carcinoma (OSCC). Abilities of glucose uptake of CAL27 and HSC3 cells were determined using glucose uptake fluorometric assay kit (E-BC-F041).

Citations

  1. PHARMACOLOGICAL RESEARCH (2022) IF: 10.334
    Sodium-Glucose Cotransporter-2 (SGLT2) expression in diabetic and non-diabetic failing human cardiomyocytes

    DOI: 10.1016/j.phrs.2022.106448

    PMID: 36096423

    Sample: AC16 cell
  2. International Journal of Environmental Research and Public Health (2022) IF: 3.39
    Benefits on Hematological and Biochemical Parameters of a High-Intensity Interval Training Program for a Half-Marathon in Recreational Middle-Aged Women Runners

    DOI: 10.3390/ijerph19010498

    PMID: 35010758

    Sample: plasma
  3. HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK (2022) IF: 3.821
    Knockdown of circGOLPH3 inhibits cell progression and glycolysis by targeting miR-145-5p/lysine demethylase 2A (KDM2A) axis in oral squamous cell carcinoma

    DOI: 10.1002/hed.27229

    PMID: 36268878

    Sample: CAL27 cells,HSC3 cells
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