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Glucose Uptake Fluorometric Assay Kit (E-BC-F041)

AllSizePriceQty
500Assays $ 2000.00
96T $ 780.00
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For research use only.

Detection Principle 2-DG is up-taken by the cells, converted to 2-DG-6P, which is catalyzed by glucose dehydrogenase to produce 6PDG. Meanwhile, NADP+ is converted to NADPH. The generated NADPH converts the probe into fluorescent substances under the action of myocardial yellow transferase. The glucose uptake can be calculated by measuring the fluorescence intensity at the excitation wavelength of 530 nm and the emission wavelength of 590 nm.
Sample Type Cell
Detection Method Fluorometric method
Detection Instrument Fluorescence microplate reader (Ex/Em=530 nm/590 nm)
Research Area Metabolic Diseases,  Glycolysis And Lipid Metabolism,  Disulfidptosis
Other Reagents Required KRPH solution (20 mM Hepes, 5 mM KH2PO4, 1 mM MgSO4, 1 mM CaCl2, 136 mM NaCl, 4.7 mM KCl, 2% BSA, pH 7.4)
Storage This product can be stored at -20°C for 12 months with shading light.
Valid Period 12 months
Assay Time 1 h 40 min
Cat.No. Product Name Sizes
E-BC-K234-M Glucose (Glu) Colorimetric Assay Kit (GOD-POD Method) 500Assays , 96T , 48T
Other Clones

1 Results

    Other Formats

    1 Results

    Biotin

    Chromogenic Biotin

    Cyanine 3

    Cyanine 5

    Cyanine 5.5

    Cyanine 7

    Elab Fluor® Violet 450

    Elab Fluor®430

    Elab Fluor®488

    Elab Fluor®568

    Elab Fluor®594

    Elab Fluor®647

    Elab Fluor®680

    Elab Fluor®700

    Elab Fluor®750

    Elab Fluor®Red 780

    Elab Fluor®Violet 450

    FITC

    HRP

    NHS-Biotin

    NHS-LC-LC-Biotin

    Sulfo-NHS-Biotin

    Sulfo-NHS-LC-LC-Biotin

    Unconjugated