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Glucose Uptake Fluorometric Assay Kit

Citations(3)

Cat.No.:E-BC-F041
Detection instrument: Fluorescence microplate reader (Ex/Em=530 nm/590 nm)

Detection method: Fluorescence method

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Detection principle

2-DG is up-taken by the cells, converted to 2-DG-6P, which is catalyzed by glucose dehydrogenase to produce 6PDG. Meanwhile, NADP+ is converted to NADPH. The generated NADPH converts the probe into fluorescent substances under the action of myocardial yellow transferase. The glucose uptake can be calculated by measuring the fluorescence intensity at the excitation wavelength of 530 nm and the emission wavelength of 590 nm.

Performance characteristics

Sample type	cells
Detection method	Fluorescence method
Assay type	Quantitative
Assay time	100 min
Other instruments required	Micropipettor, Incubator, Water bath
Other reagents required	KRPH solution (20 mM Hepes, 5 mM KH2PO4, 1 mM MgSO4, 1 mM CaCl2, 136 mM NaCl, 4.7 mM KCl, 2% BSA, pH 7.4)
Storage	-20℃
Valid period	12 months

Images

Rates of glucose consumption were significantly decreased by shRNA transfection in both kinds of cells. (*P<0.05)

T Cheng et al investigate the function of circGOLPH3 in oral squamous cell carcinoma (OSCC). Abilities of glucose uptake of CAL27 and HSC3 cells were determined using glucose uptake fluorometric assay kit (E-BC-F041).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

International Journal of Environmental Research and Public Health (2022) IF: 3.39

Benefits on Hematological and Biochemical Parameters of a High-Intensity Interval Training Program for a Half-Marathon in Recreational Middle-Aged Women Runners

DOI: 10.3390/ijerph19010498
PMID: 35010758	Sample: Plasma

HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK (2022) IF: 3.821

Knockdown of circGOLPH3 inhibits cell progression and glycolysis by targeting miR-145-5p/lysine demethylase 2A (KDM2A) axis in oral squamous cell carcinoma

DOI: 10.1002/hed.27229
PMID: 36268878	Sample: Cal27 Cells,Hsc3 Cells

PHARMACOLOGICAL RESEARCH (2022) IF: 10.334

Sodium-Glucose Cotransporter-2 (SGLT2) expression in diabetic and non-diabetic failing human cardiomyocytes

DOI: 10.1016/j.phrs.2022.106448
PMID: 36096423	Sample: Ac16 Cell

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