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Hydrogen Peroxide (H2O2) Colorimetric Assay Kit

Cat.No.:E-BC-K102-M
Detection instrument: Microplate reader(402-407 nm,optimum wavelength: 405 nm)

Detection method: Colorimetric method

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96T
48T

Price: $150

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Detection principle

Hydrogen peroxide can react with ammonium molybdate to form a yellow complex which has a maximum absorption peak at 405 nm. H2O2 content can be calculated by measuring the absorbance value at 405 nm.

Performance characteristics

Synonyms	H2O2
Sample type	Serum,plasma,urine,tissue,cells
Sensitivity	0.41 mmol/L
Detection range	0.41-125 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	30 min
Precision	Average inter-assay CV: 3.600%Average intra-assay CV: 3.200%
Other instruments required	Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Images

Electrical stimulation for more than 8 hours could lead to a significant increase of H2O2 in the media.

S Du et al investigate the promotion effect of wounds healing using programmed electric field together with minocycline. Hydrogen peroxide (H2O2) level in bacteria NB media was determined using H2O2 colorimetric assay kit (E-BC-K102-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.41-125 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Rat serum	1
Mouse serum	1
Mouse plasma	1
Porcine serum	1
Human urine	1
Cell homogenate	1
10% Plant tissue homogenate	1
10% Rat heart tissue homogenate	1
10% Rat brain tissue homogenate	1
10% Rat kidney tissue homogenate	1
10% Rat liver tissue homogenate	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Nano Energy (2021) IF: 17.881

Surface-engineered triboelectric nanogenerator patches with drug loading and electrical stimulation capabilities: Toward promoting infected wounds healing

DOI: 10.1016/j.nanoen.2021.106004
	Sample: Nutrient Broth

PHARMACOLOGICAL RESEARCH (2022) IF: 10.334

Sodium-Glucose Cotransporter-2 (SGLT2) expression in diabetic and non-diabetic failing human cardiomyocytes

DOI: 10.1016/j.phrs.2022.106448
PMID: 36096423	Sample: AC16 cell

Journal of Advanced Research (2022) IF: 10.479

Cadmium accumulation regulated by a rice heavy-metal importer is harmful for host plant and leaf bacteria

DOI: 10.1016/j.jare.2022.05.010
PMID: 35640876	Sample: leaf

MOLECULAR THERAPY (2020) IF: 8.9860

miR-204-3p/Nox4 Mediates Memory Deficits in a Mouse Model of Alzheimer’s Disease

DOI: 10.1016/j.ymthe.2020.09.006
	Sample: hippocampus

PLANT CELL AND ENVIRONMENT (2022) IF: 7.228

The rice heavy-metal transporter OsNRAMP1 regulates disease resistance by modulating ROS homoeostasis

DOI: 10.1111/pce.14263
PMID: 35040151	Sample: leaf

Frontiers in Plant Science (2022) IF: 6.627

Halotolerant Bacillus altitudinis WR10 improves salt tolerance in wheat via a multi-level mechanism

DOI: 10.3389/fpls.2022.941388
PMID: 35909740	Sample: roots or shoots

SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2022) IF: 4.052

Evaluation of testicular torsion management in Ogbomoso, South-Western Nigeria and surgical detorsion-augmented treatment with phytochemical fractions of Corchorus olitorius leaf in experimental rats

DOI: 10.1016/j.sjbs.2022.103495
	Sample: testicular tissue

Drug Design Development and Therapy (2022) IF: 4.319

The Preventive and Therapeutic Effects of Ajwa Date Fruit Extract Against Acute Diclofenac Toxicity-Induced Colopathy: An Experimental Study

DOI: 10.2147/DDDT.S344247
PMID: 35965961	Sample: serum

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020) IF: 4.556

Nox2 Upregulation and p38α MAPK Activation in Right Ventricular Hypertrophy of Rats Exposed to Long-Term Chronic Intermittent Hypobaric Hypoxia

DOI: 10.3390/ijms21228576
PMID: 33202984	Sample: Tissue homogenate

JOURNAL OF PHYSICS D-APPLIED PHYSICS (2021) IF: 3.207

Effect of pulsed electric field on diabetes-induced glycated enzyme, oxidative stress, and inflammatory markers in vitro and in vivo

DOI: 10.1088/1361-6463/ac2530
	Sample: serum

PLANT PHYSIOLOGY AND BIOCHEMISTRY (2020) IF: 3.72

Overexpression of ethylene response factor ERF96 gene enhances selenium tolerance in Arabidopsis

DOI: 10.1016/j.plaphy.2020.02.024
PMID: 32097848	Sample: Plant Tissue

Journal of Plant Interactions (2018) IF: 1.655

Brevibacillus sp. promotes maize root colonization by Acaulospora tuberculata and the alteration of associated plant protein responses

DOI: 10.1080/17429145.2018.1547844
	Sample: maize roots

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