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Cat.No.:E-BC-K046-M
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Protocols for metabolism assays
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Synonyms | LDH |
Sample type | Serum,plasma,hydrothorax,tissue,cells |
Sensitivity | 6 U/L |
Detection range | 6-500 U/L |
Detection method | Colorimetric method |
Assay type | Enzyme Activity |
Assay time | 45 min |
Precision | Average inter-assay CV: 2.4%Average intra-assay CV: 1.8% |
Other instruments required | Micropipettor, Multichannel pipette, Incubator |
Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Storage | 2-8℃ |
Valid period | 12 months |
The activity of LDH that shows the membrane leakage was significantly increased after treating with CuO. (**P<0.01 vs. Control, #P<0.05, ###P<0.001 vs. untreated group)
L A Jaragh-Alhadad et al investigates the neurotoxicity of copper oxide that may promote Alzheimer’s diseases. Lactate Dehydrogenase (LDH) activity of human cell was determined using LDH activity assay kit (E-BC-K046-M).
PMID: 35489465 |
Sample: Pig Testicular Cell (St Cell) |
Sample: Renal |
Sample: Plasma |
Sample: Serum |
PMID: 32978744 |
Sample: Serum |
Sample: Serum |
Sample: Plasma |
Sample: Mc3T3-E1 Cells |
DOI: 10.1155/2022/7832540 |
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Sample: Hepatic Tissue,Serum |
DOI: 10.1002/jez.2603 |
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Sample: Brain |
Sample: Serum |
Sample: Serum |
DOI: 10.1002/jbt.22283 |
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Sample: Serum |
PMID: 34871743 |
Sample: Serum |
Sample: Rat Thoracic Aortic Endothelial Cells (Rtaec) |
DOI: 10.1530/REP-20-0381 |
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PMID: 33112813 |
Sample: Testis |
DOI: 10.1002/jgm.3200 |
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Sample: H9C2 Cell |
DOI: 10.1039/D0RA08538C |
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Sample: Cell Sample |
Sample: Serum |
PMID: 35298730 |
Sample: Serum |
Sample: Serum |
Sample: Serum |
PMID: 35616134 |
Sample: Mle-12 Cell |
Sample: Serum,Hl-1 Cell |
PMID: 34274339 |
Sample: Serum |
DOI: 10.1002/cbin.11897 |
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Sample: Ins-1 Cell |
PMID: 33827384 |
Sample: Serum |
PMID: 36016223 |
Sample: Pbmc |
Sample: Brain |
PMID: 33971320 |
Sample: Cell Culture Supernatant |
Sample: Livers |
PMID: 36230124 |
Sample: Serum |
PMID: 35259430 |
Sample: Sh-Sy5Y Cell |
PMID: 34571217 |
Sample: Liver Organoids (Los) |
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p****pSubmitted [ Jun 06 2019 ]
N*kSubmitted [ Jun 08 2024 ]
Asked: Does it matter and affect the experiment whether I\'m using DMEM media with high or low glucose content?
Reply
adminSubmitted [ Jun 08 2024 ]
Answered: Theoretically, the glucose content in cell culture medium has little effect on the detection of LDH. However, LDH enzyme activity in cell medium is generally low, and if the medium contains phenol red, it may be affected by the detection of E-BC-K046-M kit. It is recommended that you can use E-BC-K766-M kit, which is more sensitive and easier to operation.
N************RSubmitted [ Aug 05 2023 ]
Asked: Is this kit appropriate for studying with plants (lichens)?
Reply
adminSubmitted [ Aug 05 2023 ]
Answered: We'll reply to you by email. Pls pay attention to the mailbox
M*****iSubmitted [ Oct 09 2022 ]
Asked: Why doesn\'t my standard curve go through the origin?
Reply
adminSubmitted [ Oct 09 2022 ]
Answered: Please use the absolute OD value for plotting.
M*****iSubmitted [ Aug 25 2022 ]
Asked: What is the minimum volume of sample? I\'m detecting mouse serum.
Reply
adminSubmitted [ Aug 25 2022 ]
Answered: A minimum volume of 40 uL is required.