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Mouse VEGF-A(Vascular Endothelial Cell Growth Factor A) ELISA Kit

  • Cat.No.:E-EL-M1292

  • Reactivity: Mouse

To Purchase E-EL-M1292

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse VEGF-A in samples. No significant cross-reactivity or interference between Mouse VEGF-A and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse VEGF-A concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse VEGF-A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse VEGF-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse VEGF-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse VEGF-A. You can calculate the concentration of Mouse VEGF-A in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse VEGF-A were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse VEGF-A were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 94.00 212.07 852.78 83.63 230.40 893.36
Standard deviation 4.75 10.50 46.05 4.25 11.45 47.44
CV (%) 5.05 4.95 5.40 5.08 4.97 5.31

Recovery

The recovery of Mouse VEGF-A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 97-108 103
EDTA plasma (n=8) 98-108 101
Cell culture media (n=8) 99-109 104

Linearity

Samples were spiked with high concentrations of Mouse VEGF-A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   Q00731
SynonymsVEGF-A, VEGFA, MVCD1, VPF
Research AreaCancer, Cardiovascular, Metabolism, Developmental biology, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Nature Biomedical Engineering (2021) IF: 25.671
    Lentiviral delivery of co-packaged Cas9 mRNA and a Vegfa -targeting guide RNA prevents wet age-related macular degeneration in mice

    DOI: 10.1038/s41551-020-00656-y

    PMID: 33398131

    Sample: RCS tissues
  2. CELL PROLIFERATION (2022) IF: 8.7550
    Tetrahedral framework nucleic acids promote diabetic wound healing via the Wnt signalling pathway

    DOI: 10.1111/cpr.13316

    PMID: 35869570

    Sample: skin samples
  3. GLIA (2021) IF: 7.452
    Astrocytic VEGFA: An essential mediator in blood–brain-barrier disruption in Parkinson's disease

    DOI: 10.1002/glia.24109

    Sample: primary astrocytes,pericytes
  4. DRUG DELIVERY (2022) IF: 6.419
    Sorafenib-loaded nanostructured lipid carriers for topical ocular therapy of corneal neovascularization: development, in-vitro and in vivo study

    DOI: 10.1080/10717544.2022.2048134

    Sample: corneal tissues
  5. BIOMEDICINE & PHARMACOTHERAPY (2019) IF: 6.53
    Sinomenine mitigates collagen-induced arthritis mice by inhibiting angiogenesis

    DOI: 10.1016/j.biopha.2019.108759

    PMID: 30856539

    Sample: Serum
  6. Cancers (2021) IF: 6.639
    Preclinical Evaluation of Recombinant Human IL15 Protein Fused with Albumin Binding Domain on Anti-PD-L1 Immunotherapy Efficiency and Anti-Tumor Immunity in Colon Cancer and Melanoma

    DOI: 10.3390/cancers13081789

    PMID: 33918641

    Sample: Cell sample,Serum
  7. PHYTOMEDICINE (2022) IF: 6.656
    Integration of transdermal chemistry and network pharmacology to decipher the mechanism of ShexiangZhuifeng analgesic plaster to treat rheumatoid arthritis

    DOI: 10.1016/j.phymed.2022.154507

    PMID: 36334391

    Sample: RAW264.7 cells
  8. JOURNAL OF BONE AND MINERAL RESEARCH (2018) IF: 6.741
    Positive‐Feedback Regulation of Subchondral H‐Type Vessel Formation by Chondrocyte Promotes Osteoarthritis Development in Mice

    DOI: 10.1002/jbmr.3388

    Sample: primary chondrocytes
  9. DRUG DELIVERY (2022) IF: 6.819
    Preparation and in vitro and in vivo evaluation of an isoliquiritigenin-loaded ophthalmic nanoemulsion for the treatment of corneal neovascularization

    DOI: 10.1080/10717544.2022.2096714

    PMID: 35815765

    Sample: corneal tissue
  10. Frontiers in Pharmacology (2021) IF: 5.811
    Phillyrin Prevents Neuroinflammation-Induced Blood–Brain Barrier Damage Following Traumatic Brain Injury via Altering Microglial Polarization

    DOI: 10.3389/fphar.2021.719823

    Sample: brain microvascular endothelial cells (BMECs)
  11. Frontiers in Pharmacology (2021) IF: 5.811
    Oroxylin a Attenuates Limb Ischemia by Promoting Angiogenesis via Modulation of Endothelial Cell Migration

    DOI: 10.3389/fphar.2021.705617

    PMID: 34413777

    Sample: muscle,serum
  12. Orphanet Journal of Rare Diseases (2022) IF: 4.303
    Immunological consequences of compromised ocular immune privilege accelerate retinal degeneration in retinitis pigmentosa

    DOI: 10.1186/s13023-022-02528-x

    PMID: 36253797

    Sample: serum
  13. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
    The Chinese medicine Fufang Zhenzhu Tiaozhi capsule protects against atherosclerosis by suppressing EndMT via modulating Akt1/β-catenin signaling pathway

    DOI: 10.1016/j.jep.2022.115261

    PMID: 35447198

    Sample: serum
  14. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
    Shuxuetong injection and its peptides enhance angiogenesis after hindlimb ischemia by activating the MYPT1/LIMK1/Cofilin pathway

    DOI: 10.1016/j.jep.2022.115166

    PMID: 35248678

    Sample: gastrocnemius muscles
  15. INTERNATIONAL JOURNAL OF PHARMACEUTICS (2020) IF: 4.845
    Targeted delivery of zoledronic acid through the sialic acid - Siglec axis for killing and reversal of M2 phenotypic tumor-associated macrophages – A promising cancer immunotherapy

    DOI: 10.1016/j.ijpharm.2020.119929

    Sample: Serum,Tissue homogenate
  16. Molecular Medicine Reports (2018) IF: 1.922
    VEGF secreted by mesenchymal stem cells mediates the differentiation of endothelial progenitor cells into endothelial cells via paracrine mechanisms

    DOI: 10.3892/mmr.2017.8059

    Sample: Mesenchymal stem cells (MSCs)
  17. Molecular Medicine Reports (2018) IF: 1.922
    Intraperitoneal injection of thalidomide alleviates early osteoarthritis development by suppressing vascular endothelial growth factor expression in mice

    DOI: 10.3892/mmr.2018.8980

    Sample: serum
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