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One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor® 488)

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  • Cat.No.:E-CK-A321

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Detection principle

When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.

General Information

Application DNA Fragmentation
Detection method Fluorometric Method
Sample type Paraffin section, frozen section, cell slide
Assay time 3 hours
Detection instrument Fluorescence Microscope
Dye Type Elab Fluor® 488
Ex/Em (nm) 495/519
Channel set FITC
Filter set FITC
Other reagents required 4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-IR-R187),ddH2O,Mounting solution with anti-fluorescence quencher(E-IR-R119),Xylene,Ethanol
Storage -20°C, shading light
Shipping Ice bag
Expiration date 12 months

Operation Guide

Citations

  1. JOURNAL OF NANOBIOTECHNOLOGY (2022) IF: 9.429
    Self-assembly of DNA nanostructure containing cell-specific aptamer as a precise drug delivery system for cancer therapy in non-small cell lung cancer

    DOI: 10.1186/s12951-022-01701-5

  2. Stem Cell Research & Therapy (2022) IF: 8.0790
    UC-MSCs promote frozen-thawed ovaries angiogenesis via activation of the Wnt/β-catenin pathway in vitro ovarian culture system

    DOI: 10.1186/s13287-022-02989-8

    PMID: 35841074

  3. Journal of Translational Medicine (2023) IF: 8.44
    Angiotensin-(1–7) ameliorates sepsis-induced cardiomyopathy by alleviating inflammatory response and mitochondrial damage through the NF-κB and MAPK pathways

    DOI: 10.1186/s12967-022-03842-5

  4. Journal of Inflammation-London (2022) IF: 6.283
    5-Methoxyflavone alleviates LPS-mediated lung injury by promoting Nrf2-mediated the suppression of NOX4/TLR4 axis in bronchial epithelial cells and M1 polarization in macrophages

    DOI: 10.1186/s12950-022-00319-6

  5. CELL CYCLE (2022) IF: 5.173
    Low-dose aspirin inhibits trophoblast cell apoptosis by activating the CREB/Bcl-2 pathway in pre-eclampsia

    DOI: 10.1080/15384101.2022.2092814

    PMID: 35792905

  6. PHYTOMEDICINE (2022) IF: 5.34
    Dihydromyricetin protects against Doxorubicin-induced cardiotoxicity through activation of AMPK/mTOR pathway

    DOI: 10.1016/j.phymed.2022.154027

    PMID: 35278898

  7. Journal of Translational Medicine (2022) IF: 5.531
    The role of neutrophil elastase in aortic valve calcification

    DOI: 10.1186/s12967-022-03363-1

  8. BRAIN RESEARCH BULLETIN (2022) IF: 4.079
    Sustained developmental endothelial locus-1 overexpression promotes spinal cord injury recovery in mice through the SIRT1/SERCA2 signaling pathway

    DOI: 10.1016/j.brainresbull.2022.01.016

    PMID: 35093467

  9. Toxics (2022) IF: 4.472
    Gestational Exposure to Cyfluthrin through Endoplasmic Reticulum (ER) Stress—Mediated PERK Signaling Pathway Impairs Placental Development

    DOI: 10.3390/toxics10120733

  10. Clinical and Translational Radiation Oncology (2022) IF: 4.739
    Comparison of intratumor and local immune response between MV X-ray FLASH and conventional radiotherapies

    DOI: 10.1016/j.ctro.2022.11.005

  11. Reproductive Biology and Endocrinology (2022) IF: 4.982
    hUCMSCs reduce theca interstitial cells apoptosis and restore ovarian function in premature ovarian insufficiency rats through regulating NR4A1-mediated mitochondrial mechanisms

    DOI: 10.1186/s12958-022-00992-5

    PMID: 35986315

  12. HUMAN & EXPERIMENTAL TOXICOLOGY (2023) IF: 3.247
    Sevoflurane Postconditioning Attenuates Cerebral Ischemia-Reperfusion Injury by Inhibiting SP1/ACSL4-Mediated Ferroptosis

    DOI: 10.1177/09603271231160477

  13. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY (2022) IF: 3.605
    Andrographis paniculata improves growth and non-specific immunity of shrimp Litopenaeus vannamei, and protects it from Vibrio alginolyticus by reducing oxidative stress and apoptosis

    DOI: 10.1016/j.dci.2022.104542

    PMID: 36122733

  14. NEUROSCIENCE (2023) IF: 3.708
    Peroxiredoxin-6 Released by Astrocytes Contributes to Neuroapoptosis During Ischemia

    DOI: 10.1016/j.neuroscience.2023.01.003

  15. Biopreservation and Biobanking (2021) IF: 2.3
    Effects of AavLEA1 Protein on Mouse Ovarian Tissue Cryopreservation by Vitrification

    DOI: 10.1089/bio.2021.0048

    PMID: 34788107

  16. JOURNAL OF MOLECULAR HISTOLOGY (2021) IF: 2.611
    The W9 peptide inhibits osteoclastogenesis and osteoclast activity by downregulating osteoclast autophagy and promoting osteoclast apoptosis

    DOI: 10.1007/s10735-021-10030-0

    PMID: 34664129

  17. CRYOBIOLOGY (2022) IF: 2.728
    Cryopreservation of human dental roots using vitrification for autologous human tooth tissue banking

    DOI: 10.1016/j.cryobiol.2022.11.241

  18. Experimental and Therapeutic Medicine (2022) IF: 2.751
    Dual specificity phosphatase 22 suppresses mesangial cell hyperproliferation, fibrosis, inflammation and the MAPK signaling pathway in diabetic nephropathy

    DOI: 10.3892/etm.2022.11680

  19. International Journal of Genomics (2022) IF: 2.758
    The Novel Action of miR-193b-3p/CDK1 Signaling in HCC Proliferation and Migration: A Study Based on Bioinformatic Analysis and Experimental Investigation

    DOI: 10.1155/2022/8755263

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