Introduction
Proteinase K is a kind of high activity Proteinase of subtilisin, which is used to degrade proteins in biological
samples. It can be used to digest various proteins, and it can be used in a
variety of molecular biology, cell biology and other related experiments, such
as genomic DNA extraction, enzyme digestion and removal, cell permeability and
so on.
Enzyme activity, > 30 U/mg. At 37°C, the amount of Proteinase K that can produce
amino acids or polypeptides equivalent to 1 micromol of tyrosine Folin positive
in one minute with hemoglobin as the substrate is defined as a unit of Proteinase K activity.
The effective pH range of Proteinase K is pH4.0~12.5,
and the optimal pH range is pH7.5~8.0.
The optimum reaction temperature of Proteinase K is 65°C, but at 65°C or higher, Proteinase K can also degrade
rapidly. It is suggested that the optimum reaction temperature is 50~55 °C.
Components
|
Cat.
|
Products
|
1 mL
|
5 mL
|
10 mL
|
Storage
|
|
E-IR-R109
|
Proteinase K Reagent (10 mg/mL)
|
1 mL
|
5 mL
|
10 mL
|
-20°C
|
Specification parameters
|
Source
|
Yeast
|
Appearance
|
Clear liquid
|
|
MW.
|
29 kDa
|
CAS NO.
|
39450-01-6
|
|
Purity
|
≥95% by SDS-PAGE
|
E.C
|
3.4.21.64
|
|
Activity
|
≥30U/mg
|
DNase
|
Free
|
|
Buffer
|
PBS with 10mM Tris-HCl（pH 7.5）,5mM
CaCl 2 and 50% glycerin
|
RNase
|
Free
|
Storage
Store at -20°C. Valid for 12 months.
Message submitted successfully !