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QuicKey Pro Human CRP(C-Reactive Protein) ELISA Kit

  • Cat.No.:E-OSEL-H0002

  • Reactivity: Human

To Purchase E-OSEL-H0002

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $520
Qty:

QuicKey Pro Series

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T/24
Assay time 1.5h
Detection range 125.00-8000 pg/mL
Sensitivity 52.10 pg/mL
Sample type &Sample volume serum and plasma; 50μL
Specificity This kit recognizes CRP in samples. No significant cross-reactivity or interference between CRP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of CRP concentrations in serum and plasma.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CRP. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human CRP are added to the micro ELISA plate wells. Human CRP in samples (or standards) combines with the coated antibody and HRP linked detection antibody specific to CRP. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human CRP in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
24T: 8 wells ×3 strips
96T*5: 5 plates, 96T
2-8℃, 1 months
Reference Standard 96T: 2 vials
48T/24T: 1 vial
96T*5: 10 vials
2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100×) 96T: 1 vial, 60 μL
48T/24T: 1 vial, 30 μL
96T*5: 5 vials, 60 μL
2-8℃(Protect from light)
Reference Standard & Sample Diluent 96T/48T/24T: 1 vial, 20 mL
96T*5: 5 vials, 20 mL
2-8℃
HRP Linked Ab Diluent 96T/48T/24T: 1 vial, 14 mL
96T*5: 5 vials, 14 mL
Concentrated Wash Buffer(25×) 96T/48T/24T: 1 vial, 30 mL
96T*5: 5 vials, 30 mL
Substrate Reagent 96T/48T/24T: 1 vial, 10 mL
96T*5: 5 vials, 10 mL
2-8℃(Protect from light)
Stop Solution 96T/48T/24T: 1 vial, 10 mL
96T*5: 5 vials, 10 mL
2-8℃
Plate Sealer 96T/48T/24T: 5 pieces
96T*5: 25 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level CRP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level CRP were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 386.18 1002.03 2700.34 395.70 992.83 2854.52
Standard deviation 22.59 52.91 85.87 25.25 43.39 134.73
CV (%) 5.85 5.28 3.18 6.38 4.37 4.72

Recovery

The recovery of CRP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-100 91
EDTA plasma(n=8) 91-104 98

Linearity

Samples were spiked with high concentrations of Human CRP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P02741
SynonymsC-Reactive Protein,CRP,PTX1
Research AreaCancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism, Immunology

Assay Procedures

Citations

  1. Biomedicines (2021) IF: 6.081
    Post-COVID-19 Patients Who Develop Lung Fibrotic-like Changes Have Lower Circulating Levels of IFN-β but Higher Levels of IL-1α and TGF-β

    DOI: 10.3390/biomedicines9121931

    Sample: plasma
  2. BRAIN BEHAVIOR AND IMMUNITY (2020) IF: 6.633
    Differential levels of inflammatory and neuroendocrine markers in the hippocampus and anterior cingulate cortex of bipolar disorder subjects: A post-mortem study

    DOI: 10.1016/j.bbi.2020.08.034

    PMID: 32949690

    Sample: Tissue homogenate
  3. Nutrients (2022) IF: 6.706
    Relationship between the Dietary Inflammatory Index Score and Cytokine Levels in Chinese Pregnant Women during the Second and Third Trimesters

    DOI: 10.3390/nu15010194

    Sample: serum
  4. ANALYTICA CHIMICA ACTA (2022) IF: 6.911
    A plasmonic ELISA for multi-colorimetric sensing of C-reactive protein by using shell dependent etching of Ag coated Au nanobipyramids

    DOI: 10.1016/j.aca.2022.340129

    PMID: 35934405

    Sample: AuNBP@Ag
  5. JOURNAL OF CLINICAL PERIODONTOLOGY (2020) IF: 5.241
    Clinical, inflammatory and microbiological outcomes of full‐month scaling with adjunctive glycine powder air‐polishing. A randomized trial

    DOI: 10.1111/jcpe.13400

    Sample: Serum,Gingival Crevicular Fluid
  6. WORLD JOURNAL OF GASTROENTEROLOGY (2021) IF: 5.742
    Serum soluble suppression of tumorigenicity 2 as a novel inflammatory marker predicts the severity of acute pancreatitis

    DOI: 10.3748/wjg.v27.i38.6489

    PMID: 34720537

    Sample: serum
  7. NEPHROLOGY DIALYSIS TRANSPLANTATION (2021) IF: 5.992
    Effect of fructooligosaccharide on endothelial function in CKD patients: a randomized controlled trial

    DOI: 10.1093/ndt/gfaa335

    Sample: serum
  8. NUTRITION (2021) IF: 4.008
    Changes in plasma fatty acids and related biomarkers during transition to an exclusively plant- and fish-based diet in healthy adults

    DOI: 10.1016/j.nut.2021.111306

    PMID: 34166896

    Sample: Plasma
  9. Scientific Reports (2021) IF: 4.38
    Obesity-related proteins score as a potential marker of breast cancer risk

    DOI: 10.1038/s41598-021-87583-3

    PMID: 33859244

    Sample: Plasma
  10. Nutrients (2020) IF: 4.546
    Intermittent Fasting Enhanced the Cognitive Function in Older Adults with Mild Cognitive Impairment by Inducing Biochemical and Metabolic changes: A 3-Year Progressive Study

    DOI: 10.3390/nu12092644

    PMID: 32872655

    Sample: Plasma
  11. JOURNAL OF PSYCHIATRIC RESEARCH (2022) IF: 4.791
    Comparison of cognitive impairments with lipid profiles and inflammatory biomarkers in unipolar and bipolar depression

    DOI: 10.1016/j.jpsychires.2022.04.002

    PMID: 35429740

    Sample: serum
  12. IUBMB LIFE (2015) IF: 3.143
    TSG attenuates LPC‐induced endothelial cells inflammatory damage through notch signaling inhibition

    DOI: 10.1002/iub.1458

    Sample: Cell culture supernatant
  13. CLINICAL AND EXPERIMENTAL RHEUMATOLOGY (2018) IF: 3.201
    Faecal but not serum calprotectin levels look promising in predicting active disease in Behçet's syndrome patients with gastrointestinal involvement.

    PMID: 30582504

    Sample: stool
  14. Kidney Diseases (2018) IF: 3.222
    Prognostic Significance of Serum NGAL and Troponin I against Acute Kidney Injury in Egyptian ICU Patients after Open Heart Surgery: A Pilot Study

    DOI: 10.1159/000490246

    Sample: Serum
  15. AUTOIMMUNITY (2020) IF: 2.125
    Auto-reactivity against gut bacterial peptides in patients with late-onset diabetes

    DOI: 10.1080/08916934.2020.1818232

    PMID: 32924613

    Sample: Serum
  16. BMC Neurology (2022) IF: 2.474
    Changes of biochemical biomarkers in the serum of children with convulsion status epilepticus: a prospective study

    DOI: 10.1186/s12883-022-02686-2

    PMID: 35624413

    Sample: serum
  17. Annals of Palliative Medicine (2021) IF: 2.595
    The effects of blood purification combined with antibiotics on extravascular lung water index, inflammatory factors, and prognosis of patients with severe acute pancreatitis complicated with acute respiratory distress syndrome.

    DOI: 10.21037/apm-21-2168

    Sample: blood
  18. PLoS One (2017) IF: 2.806
    High anti-human cytomegalovirus antibody levels are associated with the progression of essential hypertension and target organ damage in Han Chinese population

    DOI: 10.1371/journal.pone.0181440

    Sample: Serum
  19. JOURNAL OF CLINICAL NEUROSCIENCE (2019) IF: 1.593
    Sex difference in IL-6 modulation of cognition among Chinese individuals with major depressive disorder

    DOI: 10.1016/j.jocn.2019.10.002

    PMID: 31629608

    Sample: Serum
  20. BMC Anesthesiology (2019) IF: 1.619
    A comparison of effects of scalp nerve block and local anesthetic infiltration on inflammatory response, hemodynamic response, and postoperative pain in patients undergoing craniotomy for cerebral aneurysms: a randomized controlled trial

    DOI: 10.1186/s12871-019-0760-4

    Sample: plasm
  21. Journal of Stroke & Cerebrovascular Diseases (2020) IF: 1.787
    Incremental Accuracy of Blood Biomarkers for Predicting Clinical Outcomes After Intracerebral Hemorrhage

    DOI: 10.1016/j.jstrokecerebrovasdis.2020.105537

    PMID: 33338706

    Sample: serum
  22. European Journal of Obstetrics & Gynecology and Reproductive Biology (2020) IF: 1.868
    A comparative study of autologous rectus fascia pubovaginal sling surgery and synthetic transobturator vaginal tape procedure in treatment of women with urodynamic stress urinary incontinence

    DOI: 10.1016/j.ejogrb.2020.06.062

    Sample: blood
  23. Sport Sciences for Health (2022)
    The effects of aerobic exercise and sertraline on pro-inflammatory indices and amelioration of neurotrophic factors in patients who have undergone CABG

    DOI: 10.1007/s11332-022-00992-3

    Sample: plasma
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