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QuicKey Pro Human IgA(Immunoglobulin A) ELISA Kit

Cat.No.:E-OSEL-H0009
Reactivity: Human

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96T*5
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T/24
Assay time	1.5h
Detection range	0.78-50 ng/mL
Sensitivity	0.14 ng/mL
Sample type &Sample volume	serum, plasma, urine, saliva; 50μL
Specificity	This kit recognizes IgA in samples. No significant cross-reactivity or interference between IgA and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of IgA concentrations in serum, plasma, urine, saliva.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IgA. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human IgA are added to the micro ELISA plate wells. Human IgA in samples (or standards) combines with the coated antibody and HRP linked detection antibody specific to IgA. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human IgA in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T	2-8℃, 1 months
Reference Standard	96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials	2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100×)	96T: 1 vial, 60 μL 48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL	2-8℃(Protect from light)
Reference Standard & Sample Diluent	96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL	2-8℃
HRP Linked Ab Diluent	96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL
Concentrated Wash Buffer(25×)	96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL
Substrate Reagent	96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL	2-8℃(Protect from light)
Stop Solution	96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL	2-8℃
Plate Sealer	96T/48T/24T: 5 pieces 96T*5: 25 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level IgA were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level IgA were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	2.35	4.49	21.37	2.45	4.10	20.27
Standard deviation	0.15	0.22	0.91	0.14	0.23	1.11
CV (%)	6.41	4.95	4.25	5.69	5.66	5.48

Recovery

The recovery of IgA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	97-110	102
EDTA plasma(n=8)	88-98	93
Urine(n=8)	89-104	96

Linearity

Samples were spiked with high concentrations of Human IgA and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	Immunoglobulin A,IgA
Research Area	Cancer, Immunology

Assay Procedures

Citations

Frontiers in Microbiology (2018) IF: 4.019

Presence of Segmented Filamentous Bacteria in Human Children and Its Potential Role in the Modulation of Human Gut Immunity

DOI: 10.3389/fmicb.2018.01403
	Sample: luminal fluid

Microorganisms (2022) IF: 4.128

Effects of Inulin-Based Prebiotics Alone or in Combination with Probiotics on Human Gut Microbiota and Markers of Immune System: A Randomized, Double-Blind, Placebo-Controlled Study in Healthy Subjects

DOI: 10.3390/microorganisms10061256
PMID: 35744774	Sample: saliva samples

Frontiers in Pharmacology (2020) IF: 4.225

Adhesive Bacteria in the Terminal Ileum of Children Correlates With Increasing Th17 Cell Activation

DOI: 10.3389/fphar.2020.588560
	Sample: Ileum

Foods (2022) IF: 4.35

The Effects of Synbiotics Administration on Stress-Related Parameters in Thai Subjects—A Preliminary Study

DOI: 10.3390/foods11050759
	Sample: Serum

Foods (2021) IF: 4.35

Synbiotic Supplementation Improves Obesity Index and Metabolic Biomarkers in Thai Obese Adults: A Randomized Clinical Trial

DOI: 10.3390/foods10071580
PMID: 34359450	Sample: blood

Foods (2022) IF: 4.35

Probiotics Supplementation Improves Intestinal Permeability, Obesity Index and Metabolic Biomarkers in Elderly Thai Subjects: A Randomized Controlled Trial

DOI: 10.3390/foods11030268
PMID: 35159419	Sample: Blood

Frontiers in Physiology (2021) IF: 4.566

Human Physiological Responses to a Single Deep Helium-Oxygen Diving

DOI: 10.3389/fphys.2021.735986
	Sample: saliva

Microorganisms (2022) IF: 4.926

Evaluation of Intestinal Microbial Metabolites in Preterm Infants with Different Initial Feeding Methods by In Vitro Fermentation Modeling System

DOI: 10.3390/microorganisms10071453
PMID: 35889172	Sample: fecal slurry

MOLECULAR AND CELLULAR BIOCHEMISTRY (2021) IF: 3.396

Knockdown of RSAD2 attenuates B cell hyperactivity in patients with primary Sjögren’s syndrome (pSS) via suppressing NF-κb signaling pathway

DOI: 10.1007/s11010-021-04070-z
	Sample: Cell culture supernatant(CD19+ B cells),Serum

Journal of Infection and Public Health (2021) IF: 3.718

Prevalence of primary immunodeficiency syndromes in tuberculous meningitis: A case-control study

DOI: 10.1016/j.jiph.2021.11.019
PMID: 34883295	Sample: serum

POULTRY SCIENCE (2019) IF: 2.027

The effect of different dietary ratios of arginine, methionine, and lysine on the performance, carcass traits, and immune status of turkeys

DOI: 10.1016/j.psj.2019.10.008
PMID: 32036960	Sample: Plasma

Journal of Maternal-Fetal & Neonatal Medicine (2018) IF: 1.493

Effect of Holder pasteurization on macronutrients and immunoglobulin profile of pooled donor human milk

DOI: 10.1080/14767058.2018.1455089
	Sample: Milk

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