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Cat.No.:E-OSEL-R0003
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Assay type | Competitive-ELISA |
Format | 96T/48T/24 |
Assay time | 1.5h |
Detection range | 0.16-10 ng/mL |
Sensitivity | 0.07 ng/mL |
Sample type &Sample volume | serum and plasma; 50μL |
Specificity | This kit recognizes T in samples. No significant cross-reactivity or interference between T and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of T concentrations in serum and plasma. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T |
2-8℃, 1 months |
Reference Standard | 96T: 2 vials
48T/24T: 1 vial 96T*5: 10 vials |
2-8℃, use the reconstituted standard within 24h |
Concentrated HRP Linked Detection Ab(100×) | 96T: 1 vial, 60 μL
48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL |
2-8℃(Protect from light) |
Reference Standard & Sample Diluent | 96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL |
2-8℃ |
HRP Linked Ab Diluent | 96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL |
|
Concentrated Wash Buffer(25×) | 96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL |
|
Substrate Reagent | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃(Protect from light) |
Stop Solution | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃ |
Plate Sealer | 96T/48T/24T: 5 pieces 96T*5: 25 pieces |
|
Product Description | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level T were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level T were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (ng/mL) | 0.51 | 1.16 | 4.19 | 0.46 | 1.22 | 4.60 |
Standard deviation | 0.03 | 0.06 | 0.22 | 0.02 | 0.07 | 0.22 |
CV (%) | 5.82 | 5.07 | 5.36 | 5.37 | 5.48 | 4.74 |
The recovery of T spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 91-102 | 96 |
EDTA plasma(n=8) | 86-102 | 93 |
Samples were spiked with high concentrations of Rat T and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Synonyms | Testosterone,T |
Research Area | Signal Transduction, Signal Transduction, Epigenetics and Nuclear Signaling, Developmental Biology |
PMID: 35422283 |
Sample: serum |
PMID: 36323150 |
Sample: testis |
DOI: 10.1155/2022/2113293 |
|
Sample: serum |
PMID: 36290602 |
Sample: serum |
PMID: 34030373 |
Sample: Serum |
DOI: 10.2147/IJN.S302161 |
|
PMID: 34531655 |
Sample: serum |
PMID: 35367769 |
Sample: serum |
DOI: 10.1155/2021/7382900 |
|
Sample: serum |
PMID: 31923814 |
Sample: Tissue homogenate |
PMID: 36150640 |
Sample: plasma |
PMID: 35341824 |
Sample: serum |
PMID: 33577849 |
Sample: Tissue homogenate |
DOI: 10.1530/JME-21-0095 |
|
PMID: 34270447 |
Sample: serum |
DOI: 10.1159/000457881 |
|
Sample: Serum |
Sample: serum,intra-testicular |
PMID: 36096157 |
Sample: serum |
PMID: 34753504 |
Sample: serum |
DOI: 10.1111/jcmm.16197 |
|
PMID: 34346538 |
Sample: serum |
DOI: 10.1039/D1FO01744F |
|
PMID: 35044398 |
Sample: serum |
Sample: serum |
PMID: 33638949 |
Sample: Serum |
PMID: 35668950 |
Sample: serum |
Sample: serum |
Sample: serum |
Sample: serum |
Sample: testicular tissue |
PMID: 33581194 |
Sample: Serum |
Sample: Serum |
PMID: 32758512 |
Sample: Serum,Tissue homogenate |
Sample: serum |
PMID: 36219170 |
Sample: Plasma |
DOI: 10.1039/C9FO02554E |
|
PMID: 32441726 |
Sample: Serum |
Sample: serum |
Sample: serum |
PMID: 34229480 |
Sample: serum,testis |
PMID: 34302943 |
Sample: serum |
Sample: Serum |
PMID: 35688844 |
Sample: serum |
Sample: serum |
Sample: plasma |
Sample: plasma |
DOI: 10.3390/jcm11195584 |
|
PMID: 36233452 |
Sample: serum |
Sample: serum |
PMID: 32588312 |
Sample: Serum,Tissue homogenate |
Sample: serum |
PMID: 32812151 |
Sample: Serum,Tissue homogenate |
PMID: 34101148 |
Sample: testicular |
Sample: serum |
Sample: plasma |
PMID: 35429613 |
Sample: serum |
DOI: 10.2147/DMSO.S285025 |
|
Sample: Plasma |
Sample: serum |
PMID: 36202220 |
Sample: serum |
DOI: 10.1155/2022/4479261 |
|
PMID: 35529080 |
Sample: serum |
Sample: plasma |
Sample: Serum |
PMID: 31899225 |
Sample: Serum |
Sample: serum |
DOI: 10.1002/jbt.22445 |
|
PMID: 31975554 |
Sample: Serum |
DOI: 10.1002/jbt.22741 |
|
Sample: Serum |
Sample: serum |
PMID: 31654709 |
Sample: Serum |
DOI: 10.1530/REP-20-0316 |
|
PMID: 33434152 |
Sample: Plasma |
DOI: 10.1530/REP-20-0451 |
|
PMID: 33258800 |
Sample: Serum,Cell culture supernatant(ovarian granulosa c |
PMID: 32488017 |
Sample: Plasma,Tissue homogenate |
Sample: plasma |
Sample: Serum |
PMID: 30214533 |
Sample: Serum |
Sample: serum |
Sample: serum |
Sample: plasma |
DOI: 10.1002/tox.22559 |
|
Sample: Tissue homogenate |
Sample: serum |
DOI: 10.1002/tox.22768 |
|
Sample: serum |
DOI: 10.1002/jez.2672 |
|
Sample: serum |
DOI: 10.1111/jfbc.13615 |
|
PMID: 33491243 |
Sample: Serum |
DOI: 10.1111/and.14243 |
|
PMID: 34498746 |
Sample: serum |
DOI: 10.1111/and.14028 |
|
PMID: 33650701 |
Sample: Serum |
Sample: Serum |
DOI: 10.1111/andr.12824 |
|
Sample: Tissue homogenate |
DOI: 10.1002/jat.3764 |
|
Sample: Serum |
PMID: 30648235 |
Sample: Serum |
Sample: serum |
PMID: 30431108 |
Sample: Serum |
PMID: 30483787 |
Sample: Serum |
Sample: Serum |
Sample: Serum |
Sample: serum |
Sample: serum |
PMID: 34879886 |
Sample: serum |
Sample: Plasma |
Sample: serum |
DOI: 10.1111/jfbc.13505 |
|
Sample: Serum,Tissue homogenate |
DOI: 10.1111/jfbc.13385 |
|
Sample: Tissue homogenate |
Sample: Plasma |
DOI: 10.1155/2020/6723204 |
|
PMID: 32184896 |
Sample: Serum |
DOI: 10.1111/and.13360 |
|
Sample: plasma |
DOI: 10.1111/and.13355 |
|
Sample: serum |
DOI: 10.1111/and.13669 |
|
Sample: Sperm |
Sample: plasmsa |
Sample: plasma,placental |
Sample: plasma |
Sample: serum |
Sample: plasma |