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α-Ketoglutarate Dehydrogenase(α-KGDH) Activity Assay Kit

Cat.No.:E-BC-K083-M
Detection instrument: Microplate reader (440-460 nm, optimum wavelength: 450 nm)

Detection method: Colorimetric method

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96T

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Detection principle

α-ketoglutarate dehydrogenase (α-KGDH) is a key enzyme in the tricarboxylic acid (TCA) cycle. While the substrate being catalyzed by α-KGDH, NAD+ is reduced to NADH, and electrons are transfered to WST-8 under the action of hydrogen transmitter to produce yellow product. The activity of α-KGDH can be calculated by measuring the change of absorbance value at 450 nm

Performance characteristics

Synonyms	α-KGDH
Sample type	serum, plasma, animal and plant tissue
Sensitivity	8.3 U/L
Detection range	8.3-42.3 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	40 min
Precision	Average inter-assay CV: 8.500%Average intra-assay CV: 1.500%
Other instruments required	Incubator, Centrifuge
Storage	-20℃
Valid period	12 months

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Collection and treatment of common samples for metabolism assay detection

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