Detection of T/B/NK (3-color) in Rat Spleen - Elabscience

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Detection of T/B/NK (3-color) in Rat Spleen


Purpose

Sample

Antibody Collocation

Adjust the voltage

1

Blank

Adjust compensation

2

CD3-FITC

3

CD45RA-PE

4

CD161-APC

APC-FMO in combination with Isotype Control for auxiliary gating

5

CD3-FITC, CD45RA-PE; Mouse IgG1, κ Isotype Control-APC

PE-FMO in combination with Isotype Control for auxiliary gating

6

CD3-FITC, CD161-APC; Mouse IgG1, κ Isotype Control-PE

Full panel

7

CD3-FITC, CD45RA-PE, CD161-APC

Marker

Fluorochrome

Clone No.

Cat. No.

CD3

FITC

G4.18

E-AB-F1228C

CD45RA

PE

OX-33

E-AB-F1306D

CD161

APC

3.2.3

E-AB-F1307E

Mouse IgG1, κ Isotype Control

PE

MOPC-21

E-AB-F09792D

Mouse IgG1, κ Isotype Control

APC

MOPC-21

E-AB-F09792E


Tips:

1. The phenotype of rat B cells is CD3-CD45RA+, and the phenotype of NK cells is CD3-CD161+.

2. Isotype Control for CD45RA and CD161 is suggested for auxiliary gating.




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