Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope. Nuclear dyes (PI, DAPI, 7-AAD) have a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be distinguished.

General Information

Application	Annexin V
Detection method	Fluorometric Method
Sample type	Cell samples
Assay time	40min
Detection instrument	Flow Cytometer
Dye Type	APC
Ex/Em (nm)	650/660
Channel set	APC
Other reagents required	PBS(E-BC-R187), Deionized water
Storage	2~8°C, shading light
Shipping	Ice bag
Expiration date	12 months

Related Products

E-CK-A151	Annexin V Binding Buffer (10×)
E-CK-A117	Annexin V-APC Reagent
E-CK-A218	Annexin V-APC/7-AAD Apoptosis Kit
E-CK-A258	Annexin V-APC/DAPI Apoptosis Kit
E-CK-A320	One-step TUNEL In Situ Apoptosis Kit (Green, FITC)
E-CK-A301	Mitochondrial Membrane Potential Assay Kit (with JC-1)

Citations

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International Journal of Laboratory Hematology (2021) IF: 2.877

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