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Cat.No.:E-BC-K353-S
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Synonyms | APX |
Sample type | Plant tissue |
Sensitivity | 0.071 U/g tissue |
Detection range | 0.071-47 U/g tissue |
Detection method | Colorimetric method |
Assay type | Enzyme Activity |
Assay time | 60 min |
Precision | Average inter-assay CV: 6.400%Average intra-assay CV: 4.800% |
Other instruments required | Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge |
Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Storage | 2-8℃ |
Valid period | 12 months |
In high light stress situation, the APX activity was significantly reduced in snat1 mutant group. (P<0.05)
H Y Lee et al investigate the effects of melatonin in high light stress tolerance in Arabidopsis thaliana. Ascorbate peroxidase (APX) activity of Arabidopsis leaf was determined using APX activity assay kit (E-BC-K353-S).
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.071-47 U/g tissue).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
10% Epipremnum aureum tissue homogenization |
1 |
10% Carrot tissue homogenization |
1 |
10% Green pepper tissue homogenization |
1 |
10% Mushrooms tissue homogenization |
1 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
PMID: 33962063 |
Sample: Plant Tissue |
DOI: 10.1111/jpi.12504 |
|
PMID: 29770489 |
Sample: Leaf tissue |
PMID: 35909740 |
Sample: roots or shoots |
PMID: 32097848 |
Sample: Plant Tissue |
Sample: leaf |
DOI: 10.7717/peerj.11888 |
|
PMID: 34434650 |
Sample: seedling |
Sample: seed |
M*****aSubmitted [ Oct 10 2022 ]
Asked: I bought K353-S (APX kit) and K259-M (PPO kit), could the homogenate medium be universal?
Reply
adminSubmitted [ Oct 10 2022 ]
Answered: No, both kits need to use the homogenate of reagent 1 in the corresponding kit, not mixed, and not replaced with anything else.
M*****aSubmitted [ Sep 27 2022 ]
Asked: I bought K353-S (APX kit) and K259-M (PPO kit), could the homogenate medium be universal?
Reply
adminSubmitted [ Sep 27 2022 ]
Answered: No, both kits need to use the homogenate of reagent 1 in the corresponding kit, not mixed, and not replaced with anything else.
M*****aSubmitted [ Sep 08 2022 ]
Asked: Same thing to reagent 3 of E-BC-K353-S, it ask to dissolve whole pack of powder, how do i keep part of it?
Reply
adminSubmitted [ Sep 08 2022 ]
Answered: For reagent3 of E-BC-K353-S, it's powder, so once it's dissolved, it needs to be used up within 3 days.