Butyl S-6FF can interact with some hydrophobic protein or antibody under high ionic strength conditions (high ionic strength may increase the interaction between ligand and hydrophobic groups), thus to achieve the purpose of separation and purification. Butyl S-6FF is mainly used for capture of initial samples and moderate and fine purification of samples.

Product Features

1. Rapid, easy to use (one-step purification).
2. Compared with reversed-phase chromatography, the ligand concentration in the hydrophobic interaction chromatography media is low and the elution conditions are mild, which helps to maintain the biological activity of biomolecules.
3. Wide application. Butyl S-6FF can be applied in preliminary capture, moderate purification and fine purification, and it can be repeatedly used in combination ion exchange chromatography media.

Product Datasheet

Matrix	Highly cross-linked 6% agarose
Particle size range	45-65 µm
Average particle size	90 µm
Binding capacity	10-20 mg(Lysyme)/mL(media)
Ligand	Butyl-S
Ligand density	10 µmol/mL
Media type	Hydrophobic Interaction Medium
pH stability	2-14(short-term) 3-13(long-term)
Chemical stability	All of the commonly used buffers, 1M Acetic acid, 1M NaOH, 8M urea, 6M guanidine hydrochloride, 30% Isopropyl alcohol, 70% Ethanol
Maximum flow rate	450 cm/h
Storage buffer	20% Ethanol
Storage temperature	4~30℃(4~8℃ is preferred)

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Cat. No	E-CM-HI06
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