CFDA SE is a kind of fluorescein dye with membrane permeability, which has no fluorescence itself. When CFDA SE enters living cells through the cell membrane, it can be catalyzed by esterase in cytoplasm to produce carboxyfluorescein succinimide ester (CFSE), which can emit strong green fluorescence, can not penetrate the cell membrane, and can remain intact in the cell. CFSE can also spontaneously and irreversibly combine with amino groups of intracellular proteins to couple to these proteins. At the same time, excessive and uncoupled CFDA SE is passively diffused back into extracellular medium and removed by subsequent washing steps.
The fluorescence of CFDA SE-labeled cells is uniform and stable. In the process of cell division and proliferation, the fluorescence of CFDA SE-labeled cells could be evenly distributed to two progeny cells, and the fluorescence intensity becomes half of the parental cells. According to the difference of fluorescence intensity, the detection results can be divided into undivided cells, cells that have split once (1/2 fluorescence intensity), cells that have split twice (1/4 fluorescence intensity), cells that have split three times (1/8 fluorescence intensity), and cells that have split more times. CFDA SE-labeled cells can be used for in vitro and in vivo proliferation studies without staining of neighboring cells. CFDA SE-labeled cells showed green fluorescence, Ex=494 nm, Em=521 nm, which can be detected by flow cytometry or fluorescence microscope.
Message submitted successfully !