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Glycerol-3-phosphate (G3P) Fluorometric Assay Kit

  • Cat.No.:E-BC-F081

  • Detection instrument: Fluorescence microplate reader (Ex/Em=535 nm/587 nm)

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  • 96T
  • 48T
Price: $520
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Detection principle

Glycerol 3-phosphate (G3P), also known as 3-glycerophosphoic acid and glycerol α-phosphate, is mainly derived from glycolysis. G3P is an important intermediate in the glycerol-3-phosphate shuttle mechanism and studies have shown that G3P is involved in electron transport, and its metabolic level is related to lipid metabolism, insulin resistance and some cancers. The principle of this kit is that G3P is decomposed by enzymes, and its products generate fluorescent substances with probes under the action of other enzymes. The higher the fluorescence value, the higher the G3P content in the sample.

Performance characteristics

Synonyms G3P
Sample type tissue;cell
Sensitivity 0.19 μmol/L
Detection range 0.19-100 μmol/L
Detection method Fluorescence method
Assay type Quantitative
Assay time 30 min
Precision Average inter-assay CV: 4.6-10.0%Average intra-assay CV: 0.7-2.8%
Other instruments required Incubator(37℃), 3 kD ultrafiltration tube
Other reagents required Normal saline (0.9% NaCl), Double distilled water
Storage -20℃
Valid period 12 months

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